Method for efficiently separating and purifying Diosmin

A separation, purification, and high-efficiency technology, applied in chemical instruments and methods, organic chemistry, preparation of sugar derivatives, etc., can solve the problems of low separation purity and high import price of Diosmin, and achieve favorable promotion and application, good economic benefits, The effect of the mature process route

Inactive Publication Date: 2016-06-22
HARBIN INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for efficiently separating and purifying Diosmin in order to solve the problems of low separation purity of existing Diosmin and high import price

Method used

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  • Method for efficiently separating and purifying Diosmin
  • Method for efficiently separating and purifying Diosmin
  • Method for efficiently separating and purifying Diosmin

Examples

Experimental program
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Effect test

specific Embodiment approach 1

[0017] Specific embodiment one: a kind of method of efficient separation and purification of Diosmin of the present embodiment is carried out according to the following steps:

[0018] 1. Sample pretreatment: Take the dried cape japonica, extract twice with 70% ethanol solution under reflux, each time for 1.5h-2.5h, the ratio of solid to liquid for the first extraction is 1:10, the second time The solid-liquid ratio of the extraction is 1:8, the 2 extractions are filtered, the ethanol solution is recovered by concentration under reduced pressure, and the extract is obtained;

[0019] Two, one-time separation and purification: the medicinal extract obtained in step 2 is dissolved in water to obtain a first-time sample solution, and the concentration of the extractum in the first-time sample solution is 800mg / L~900mg / L. AB-8 macroporous adsorption resin column for sample adsorption, the adsorption conditions are: temperature 15 ~ 25 ℃, flow rate 1.5BV / h ~ 2.5BV / h, the amount of ...

specific Embodiment approach 2

[0021] Specific embodiment two: the difference between this embodiment and specific embodiment one is: in step one, take the dried vetch, and use a concentration of 70% ethanol solution to reflux and extract twice, each time for 2 hours. Other steps and parameters are the same as those in the first embodiment.

specific Embodiment approach 3

[0022] Specific embodiment three: this embodiment is different from specific embodiment one or two: the preparation method of the AB-8 macroporous adsorption resin column described in step 2 is as follows: 1. get AB-8 macroporous adsorption resin with a concentration of 95 % ethanol solution for 24 hours to swell, then place the swollen AB-8 macroporous adsorption resin in a round bottom flask, reflux and elute with 95% ethanol solution for 4 hours to 6 hours, and then carry out wet column packing; ②After loading the column, use 95% ethanol solution to pass through the resin layer at a flow rate of 1.5BV / h~2.5BV / h until the effluent is not white and turbid after adding water; ③Wash through the resin layer with water at a flow rate of 2BV / h ethanol solution; ④ rinse with 5% HCl solution at a volume of 4BV to 6BV and a flow rate of 4BV / h to 6BV / h, and then wash with distilled water until the pH of the effluent is 6 to 7; The NaCl solution is rinsed with a volume of 4BV-6BV and a...

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Abstract

The invention relates to a method for efficiently separating and purifying Diosmin and aims to solve the problem that existing Diosimin separate is low in purity and high in import price. The method includes: first, pretreating a sample; second, separating and purifying firstly; third, separating and purifying secondily. Macroporous resin is enriched and separated in Galium verum, Diosmin (XIANGYE MUGAN) high in content and purity is purified, a process route is mature, the cost is low, a solvent has low toxic and side effects, the recycling rate is high, and the method is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for efficiently separating and purifying Diosmin. Background technique [0002] Diosmin (also known as diosmin) is a flavonoid glycoside compound with strong physiological functions, which can enhance the tension of veins and treat related diseases such as venous lymphatic insufficiency; it can also reduce microcirculation stasis, improve lymphatic return, and reduce edema , has the effect of treating postoperative edema, diabetic complications, prostatic hyperplasia and common anorectal diseases; at the same time, diosmin also has the antioxidant activity of most flavonoids, and it has been found in experiments that it has a strong effect on oxidative damage caused by radiation The repairing effect; In recent years, it has attracted much attention in cancer prevention and treatment. [0003] At present, Diosmin used internationally is a synthetic product. The domestic separation and purification process is immature, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61P7/04
CPCC07H17/07C07H1/08
Inventor 卢卫红张红
Owner HARBIN INST OF TECH
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