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Method for improving lutein yield through bacterium and alga co-culture

A technology of co-cultivation and lutein, which is applied in the field of lutein preparation and extraction, can solve the problems of low production efficiency of algae cells and high cost of lutein preparation, and achieve a significant increase in lutein production rate and significant benefits Effect

Inactive Publication Date: 2016-06-22
ZHEJIANG GONGSHANG UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0007] Aiming at the key technical limitations of low algae cell production efficiency and high lutein preparation cost in the current industrial production of chlorella, the present invention develops a method for promoting the growth rate of chlorella and increasing the production of lutein by applying Pantobacter oryzae endophytes. Co-cultivation technology of artificial bacteria and algae

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  • Method for improving lutein yield through bacterium and alga co-culture
  • Method for improving lutein yield through bacterium and alga co-culture

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Embodiment 1

[0029] 1) The rice endophytic Pantoea strains were purchased from the China Agricultural Microbiology Collection Center (ACCC), and the code is 10454. After the strains were activated by conventional methods, they were inoculated into a Erlenmeyer flask containing 10mL of LB liquid medium for expansion. For expansion culture, the bacteria liquid in the logarithmic growth phase after the expansion culture for 16 hours is inoculated according to the volume ratio of bacteria liquid: liquid LB medium = 1:10, and then the second expansion culture is carried out. The culture conditions are 37°C, 200rpm constant temperature shaker dark culture. Continuous expansion was carried out three times, and the culture conditions were 37°C, 200rpm constant temperature shaker and dark culture. The third expansion culture 10h to OD 600 =1 is used as the seed cell of the co-cultivation of bacteria and algae. According to the growth curve, the growth of the bacteria is in the logarithmic phase. T...

Embodiment 2

[0043] In order to verify the technical effect of the artificial bacteria and algae co-cultivation technology of the present invention on improving the Chlorella lutein output, operate as follows:

[0044] 1) The algae cells and bacteria and algae cell dry products harvested from the above-mentioned embodiment one pure algae culture control group (CA and CB) and the corresponding bacteria and algae co-cultivation test group (A and B), each accurately weighed 0.1g of algae powder, According to the ratio of material to liquid (g:ml) = 1:40, add 4ml of extract solution (methanol: dichloromethane volume ratio = 2:1), and put it in a tank-type ultrasonic generator water bath in a 320W power water bath at a room temperature of 25°C Treat the broken cells for 10 minutes, rest for 5 minutes, break the cells for 10 minutes, then put them in a constant temperature water tank at 40°C for 10 minutes, then centrifuge at 6000r / min for 10 minutes, and collect the supernatant lutein extract an...

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Abstract

The invention discloses a method for improving the lutein yield through bacterium and alga co-culture. The method includes the steps of firstly, conducting the first round of bacterium and alga co-culture on rice endogenous generic bacteria and chlorella under illumination, and obtaining a first-round bacterium and alga co-culture solution after culture ends; secondly, adding rice endogenous generic bacteria to the first-round bacterium and alga co-culture solution, conducting the second round of bacterium and alga co-culture under illumination, and obtaining a second-round bacterium and alga co-culture solution after culture ends; thirdly, centrifuging the second-round bacterium and alga co-culture solution to obtain bacterium and alga cell precipitate, and conducting post-treatment to obtain a bacterium and alga cell dry product; fourthly, smashing the bacterium and alga cell dry product to obtain bacterium and alga powder, and extracting lutein through an organic solvent extraction solution to obtain lutein. By conducting bacterium and alga co-culture through rice endogenous generic bacteria and chlorella, the growth speed and biological amount of chlorella cells are increased, the content of lutein in alga cells is increased, and remarkable effects and wide prospects are achieved on the aspect of application and development of chlorella.

Description

technical field [0001] The invention relates to the field of preparation and extraction of lutein, in particular to a method for increasing the yield of lutein through co-cultivation of bacteria and algae. Background technique [0002] Lutein, also known as lutein, is one of the oxygen-containing carotenoids-xanthophyl, which widely exists in plants such as flowers, fruits and vegetables, as a natural yellow pigment, Lutein can be used as a pigment additive in food, medicine and cosmetics. In addition, lutein can stimulate the immune response, improve the body's immunity, and prevent vision loss and blindness caused by age-related macular degeneration. 31(01):268-273). The United States began to extract lutein from marigolds in the 1970s. In 1995, the U.S. Food and Drug Administration (FDA) approved lutein as a food supplement for food and beverages. The Ministry of Health of my country also approved the use of lutein in baked food, beverages, jelly, jam and frozen food i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P23/00C12R1/89C12R1/01
CPCC12P23/00C12P39/00
Inventor 赵艳
Owner ZHEJIANG GONGSHANG UNIVERSITY