Gastric cancer sialoprotein fingerprint spectrum molecular diagnosis module building method
A fingerprint and salivary protein technology, applied in the field of molecular diagnostic model establishment of gastric cancer salivary protein fingerprint, can solve the problems of low sensitivity, long detection cycle, complex detection of gastric cancer, etc., and achieve high diagnostic efficiency
Active Publication Date: 2016-06-22
SHENZHEN ELDERLY MEDICAL RES INST +1
View PDF3 Cites 6 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
[0006] In view of this, the present invention aims to solve the problems of complex detection of gastric cancer, long detection period, low sensitivity and poor specificity in the prior art, and provides a method for establishing a molecular diagnostic model of gastric cancer salivary protein fingerprint
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0036] 1 case selection
[0037] 1.1 Diagnostic criteria
[0038] The diagnosis of gastric cancer and chronic gastritis (including chronic superficial gastritis and chronic atrophic gastritis) is based on the diagnostic criteria of "Internal Medicine".
[0039] 1.2 Inclusion criteria
[0040] (1) Meet the diagnostic criteria and be between 20 and 75 years old;
[0041] (2) Without surgery and chemotherapy;
[0042] (3) Subjects who are voluntary and able to cooperate to participate.
[0043] All three of the above items must be met in order to be included.
[0044] 1.3 Exclusion criteria
[0045] (1) Those who do not meet the above diagnostic criteria;
[0046] (2) Those who are 75 years old;
[0047] (3) Suffering from local oral cavity and salivary gland inflammation, peptic ulcer, tumor and congenital diseases; suffering from Sjögren's syndrome, cystic fibrosis; suffering from other serious concurrent diseases.
[0048] 1.4 Quality Control
[0049] (1) Adopting uni...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
| Property | Measurement | Unit |
|---|---|---|
| Sensitivity | aaaaa | aaaaa |
Login to View More
Abstract
The invention discloses a gastric cancer sialoprotein fingerprint spectrum molecular diagnosis module building method. The method comprises the following steps of sample collection, nanometer magnetic bead pretreatment, sample pretreatment, saliva sample elution sample loading, mass spectrometry analysis, data collection, data analysis and diagnosis module building. The WCX is combined with an MALDI-TOF-MS protrinology technology, the advantages of high flux, high sensitivity and high qspecificity are realized in an aspect of technology; the differences of gastric cancer and chronic gastritis patient saliva on the proteome level are analyzed; 23 difference protein peaks with the significance are discovered; an identification diagnosis model formed by three obvious difference protein peaks of 4267.09, 6564.85 and 2138.14m / z; through clinic back substitution inspection, the sensitivity of the model reaches 96 percent, and the specificity of the model reaches 86 percent; the result shows that the model has higher diagnosis efficiency on the identification of the gastric cancer and the chronic gastritis.
Description
technical field [0001] The invention belongs to the technical field of protein spectrum application, and in particular relates to a method for establishing a molecular diagnosis model of gastric cancer salivary protein fingerprint. Background technique [0002] As we all know, the early clinical manifestations of gastritis and gastric cancer are difficult to distinguish, so the differential diagnosis of early gastric cancer and chronic gastritis is particularly important. The gold standard for the diagnosis of gastric cancer is endoscopy plus biopsy, but this examination is an invasive examination, and patients generally experience strong pain during the examination. Moreover, a large number of gastric cancer patients have no obvious symptoms in the early stage, and the initiative to seek medical advice is poor, so it is difficult to be used as a general screening method for gastric cancer; The examination of prosecretin (PG) level plays an important role in the diagnosis of...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/62
CPCG01N33/6851
Inventor 吴正治黄飞娟曹美群孙珂焕谢梦洲贺佐梅
Owner SHENZHEN ELDERLY MEDICAL RES INST