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Spirulina amino acid small peptide and microelement chelate additive and application thereof

A technology of trace elements and spirulina, which is applied in application, animal feed, additional food elements, etc., can solve the antagonism effect of pH value in the body, inorganic ions, organic macromolecules, etc., destroy the nutritional content of feed, and insufficient preparation technology To improve the ability to resist emergencies, accelerate the growth of animals, and improve immunity

Inactive Publication Date: 2016-06-29
GUANGZHOU JUZHU GENERAL TECH INST CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the trace element feed additives on the market mainly include trace element inorganic salts, trace element simple organic acid salts and trace element amino acid chelates. Antagonism effects such as organic macromolecules; large gastrointestinal irritation to animals, will destroy other nutrients in feed; low digestion and absorption rate, high content of heavy metals in excrement, and great environmental pollution
Trace element amino acid chelate overcomes the shortcomings of the previous two generations of trace element additives, but its application is limited due to the lack of amino acid sources, high cost, and immature preparation technology

Method used

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  • Spirulina amino acid small peptide and microelement chelate additive and application thereof
  • Spirulina amino acid small peptide and microelement chelate additive and application thereof
  • Spirulina amino acid small peptide and microelement chelate additive and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] a. Take 20 parts of spirulina powder in distilled water, stir evenly until the final concentration is 15% (W / V), then repeatedly freeze and thaw 5 times at -16°C and 45°C, and then ultrasonicate for 10s at intervals of 15s at 600W ultrasonic power , sonicated 90 times in total, and then centrifuged the obtained solution at 4°C and 6000r / min for 40min to obtain the spirulina protein supernatant;

[0033] b. Adjust the pH value of the above-mentioned spirulina protein supernatant to 5.5, raise the temperature to 45°C, add 2% plant protease for hydrolysis for 4 hours, and stir once every 40 minutes during this period;

[0034] c. Put the hydrolyzate of the spirulina protein supernatant in a 98°C incubator for 25 minutes to inactivate the enzyme, then centrifuge at 3500r / min for 30 minutes, collect the clarified extract, and obtain the spirulina amino acid small peptide mixture;

[0035] d. Take 6 parts by weight of the trace element inorganic salt selenium nitrate, dissolv...

Embodiment 2

[0038] a. Take 35 parts of spirulina powder in distilled water, stir evenly until the final concentration is 17% (W / V), then freeze and thaw repeatedly at -16°C and 45°C for 5 times, and then ultrasonicate for 10s at intervals of 15s at 600W ultrasonic power , sonicated 90 times in total, and then centrifuged the obtained solution at 4°C and 6000r / min for 40min to obtain the spirulina protein supernatant;

[0039] b. Adjust the pH value of the above-mentioned spirulina protein supernatant to 7.8, raise the temperature to 55°C, add 3.2% neutral protease to hydrolyze for 9 hours, and stir once every 45 minutes during this period;

[0040] c. Put the hydrolyzate of the spirulina protein supernatant in a 98°C incubator for 25 minutes to inactivate the enzyme, then centrifuge at 3500r / min for 30 minutes, collect the clarified extract, and obtain the spirulina amino acid small peptide mixture;

[0041] d. Weigh 10 parts by weight of manganese nitrate, an inorganic salt of trace elem...

Embodiment 3

[0044] a. Take 50 parts of spirulina powder in distilled water, stir evenly until the final concentration is 20% (W / V), then freeze and thaw repeatedly at -16°C and 45°C for 5 times, and then ultrasonicate for 10s at intervals of 15s at 600W ultrasonic power , sonicated 90 times in total, and then centrifuged the obtained solution at 4°C and 6000r / min for 40min to obtain the spirulina protein supernatant;

[0045] b. Adjust the pH value of the above-mentioned spirulina protein supernatant to 8.0, raise the temperature to 65°C, add 4.5% neutral protease to hydrolyze for 9 hours, and stir once every 50 minutes during this period;

[0046] c. Put the hydrolyzate of the spirulina protein supernatant in a 98°C incubator for 25 minutes to inactivate the enzyme, then centrifuge at 3500r / min for 30 minutes, collect the clarified extract, and obtain the spirulina amino acid small peptide mixture;

[0047] d. Weigh 15 parts by weight of trace element inorganic salt ferrous nitrate, dissol...

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Abstract

The invention discloses a spirulina amino acid small peptide and microelement chelate additive and application thereof. Spirulina serves as an amino acid small peptide source of the additive. Firstly, spirulina protein is extracted with a multigelation and ultrasonication method; then, spirulina protein is subjected to enzymolysis, enzymatic hydrolysate is mixed with microelement inorganic salt and plant feed in sequence, the mixture is dried, and the spirulina amino acid small peptide and microelement chelate additive is obtained. Spirulina extremely easy to breed is fully utilized, so that the spirulina amino acid small peptide and microelement chelate additive is safe, low in cost and good in palatability, enhances the immunity of animals while increasing the microelement utilization rate of animals and relieves pollution caused by a large quantity of heavy metal residues in excrement to the environment.

Description

technical field [0001] The invention belongs to the technical field of feed additives, in particular to a spirulina amino acid small peptide trace element chelate additive and application thereof. Background technique [0002] Trace elements are one of the essential nutritional elements for animals, which directly or indirectly participate in almost all physiological and biochemical processes of the body. Although the content of trace elements in animal feed is very small, they play a very important role in the growth and development of animals, production and reproduction, and resistance to diseases. [0003] The research and application of trace elements as feed additives has always been one of the hot topics in the field of animal nutrition. At present, the trace element feed additives on the market mainly include trace element inorganic salts, trace element simple organic acid salts and trace element amino acid chelates, but the first two trace element additives have po...

Claims

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Application Information

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IPC IPC(8): A23K20/147A23K20/20A23K50/75
Inventor 杨广群曹丽萍
Owner GUANGZHOU JUZHU GENERAL TECH INST CO LTD
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