Method for producing microalgal oil by using flue gas

A technology of microalgae oil and flue gas, which is applied in the fields of biotechnology and bioenergy, can solve the problems of low efficiency, achieve the effects of increasing growth rate, promoting rapid growth, alleviating the greenhouse effect and exhaust pollution problems

Active Publication Date: 2016-06-29
CHINA PETROLEUM & CHEM CORP +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] For existing algal species that cannot tolerate and absorb high concentrations of CO 2 , fixed CO 2 Th

Method used

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  • Method for producing microalgal oil by using flue gas
  • Method for producing microalgal oil by using flue gas

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0026] Example 1

[0027] (1) In a 10L photobioreactor, add the Scenedesmus obliquus FSH-Y2 seed solution and microalgae culture medium prepared in Example 1. The added amount of FSH-Y2 seed solution is 400 mL, and the pH value of the microalgae culture medium Adjust to 10, add 8L, culture light intensity to 5000 Lux, photoperiod 24h, light-dark time ratio 14:10, and let CO in the flue gas 2 The content is 3v%, NO and NO 2 The content is 50×10 -6 (V / v), SO 2 The content is 60×10 -6 (V / v).

[0028] (2) After culturing for 4 days, connect to the seed solution of Monophyta SS-B1 and the seed solution of Cellulosae SS-B7 prepared in Example 1. The added amount of the two kinds of algae seed solution is 200 mL, adjust the pH of the microalgae culture system Is 8, continuous light cultivation, the light intensity is 5000 Lux; let CO in the flue gas 2 The content is 30v%, NO and NO 2 The content is 500×10 -6 (V / v), SO 2 The content is 600×10 -6 (V / v).

[0029] (3) After culturing for 7 day...

Example Embodiment

[0030] Example 2

[0031] (1) In a 10L photobioreactor, add Scenedesmus obliquus FSH-Y2 seed solution and microalgae culture medium prepared in Example 1. The added amount of FSH-Y2 seed solution is 800mL, and the pH value of the microalgae culture medium Adjust to 12, add 8L, cultivate light intensity of 5000 Lux, photoperiod 24h, light-dark time ratio 14:10, and let CO in the flue gas 2 The content is 5v%, NO and NO 2 The content is 80×10 -6 (V / v), SO 2 The content is 100×10 -6 (V / v).

[0032] (2) After culturing for 2 days, connect to the seed solution of Monophyta SS-B1 and Cellulosae SS-B7 prepared in Example 1. The added amount of the two kinds of algae seed solution is 200 mL, and the pH of the microalgae culture system is adjusted. It is 9, continuous light cultivation, the light intensity is 5000 Lux; the CO in the flue gas is introduced 2 The content is 30v%, NO and NO 2 The content is 500×10 -6 (V / v), SO 2 The content is 600×10 -6 (V / v).

[0033] (3) After 8 days of cultu...

Example Embodiment

[0034] Example 3

[0035] (1) In a 10L photobioreactor, add Scenedesmus obliquus FSH-Y2 seed solution and microalgae culture medium prepared in Example 1. The amount of FSH-Y2 seed solution added is 600 mL, and the pH value of the microalgae culture medium Adjust to 11, add 8L, cultivate light intensity of 5000 Lux, photoperiod 24h, light-dark time ratio 14:10, and let CO in the flue gas 2 The content is 4v%, NO and NO 2 The content is 50×10 -6 (V / v), SO 2 The content is 60×10 -6 (V / v).

[0036] (2) After culturing for 3 days, connect the seed solution of Mononeous algae SS-B1 and Cellulosae SS-B7 prepared in Example 1. The added amount of the seed solution of both algae is 300mL to adjust the microalgae culture system The pH is 9, the continuous light cultivation, the light intensity is 5000 Lux; the CO in the flue gas is introduced 2 The content is 40v%, NO and NO 2 The content is 500×10 -6 (V / v), SO 2 The content is 600×10 -6 (V / v).

[0037] (3) After 8 days of culture, it enters...

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Abstract

The invention discloses a method for producing microalgal oil by using flue gas, wherein the method includes the steps: (1) adding a microalgae culture medium and a scenedesmus obliquus FSH-Y2 seed liquid into a photobioreactor, regulating the pH of the culture system to 10-12, introducing flue gas with the CO2 volume content of 1 v%-5 v%, and culturing for 2-5 days; and (2) regulating the pH value of the culture system to 8-10, inoculating with monoraphidium sp. SS-B1 and ankistrodesmus sp. SS-B7 seed liquids, carrying out mixed culture, introducing flue gas with the CO2 volume content of 5 v%-45 v%, culturing to a stable stage under continuous illumination, and harvesting microalgae cells, wherein the scenedesmus obliquus FSH-Y2, the monoraphidium sp. SS-B1 and the ankistrodesmus sp. SS-B7 are respectively preserved in China General Microbiological Culture Collection Center on September 11, 2012 and on April 15, 2013 respectively and have the preservation numbers of CGMCC No.6551, CGMCC No.7479 and CGMCC No.7478 respectively. The method improves the tolerance and the solubility of the microalgae culture system on high-concentration CO2, improves the carbon sequestration efficiency, obviously increases the yield of the microalgal oil, and can realize purification of the flue gas.

Description

technical field [0001] The invention belongs to the fields of biotechnology and bioenergy, and in particular relates to a method for producing microalgae oil by using flue gas. Background technique [0002] Due to the decrease of fossil energy and the increase of greenhouse effect caused by the use of fossil energy, more and more scientific researchers focus on the development and utilization of renewable energy. As the most important renewable energy on earth, biomass energy includes forestry biomass, crops, aquatic plants, agricultural waste, etc. Among many biomass energy sources, microalgae is an important renewable resource. They have the characteristics of wide distribution, large biomass, high photosynthetic efficiency, strong environmental adaptability, short growth cycle, and high biomass yield. Its cells contain unique primary or secondary metabolites with complex chemical composition. The solar energy conversion efficiency of microalgae can reach 3.5%, which is...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/64C12R1/89
Inventor 师文静廖莎高大成孙启梅李晓姝姚新武樊亚超王领民
Owner CHINA PETROLEUM & CHEM CORP
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