Method for preparing abalone harslet polysaccharide powder for removing proteins and heavy metals
A technology for removing heavy metals and proteins, applied in the field of seafood research, can solve the problems of high protein content, achieve high purity, low requirements, and easy operation
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Embodiment 1
[0027] A preparation method of abalone viscera polysaccharide powder for removing protein and heavy metal, which specifically comprises the following steps:
[0028] (1) Preparation of crude polysaccharide solution of abalone viscera: thaw frozen abalone viscera naturally, mix 1 kg of abalone viscera with 1 kg of water, beat with a small single-channel beater, and separate and remove the connective tissue in the abalone viscera tissue to obtain abalone viscera fluid; then mechanically separate the obtained abalone viscera fluid to remove water-insoluble substances to obtain crude abalone viscera polysaccharide solution;
[0029] (2) Enzymolysis: Use 0.5 mol / L sodium hydroxide solution to adjust the pH value of the crude abalone viscera polysaccharide solution obtained in step (1) to 5, add 3% papain by weight of the crude abalone viscera polysaccharide solution, and homogenize After 2 times, heat in a constant temperature water bath at 45°C for 2 hours to obtain an enzymatic h...
Embodiment 2
[0040]A preparation method of abalone viscera polysaccharide powder for removing protein and heavy metal, which specifically comprises the following steps:
[0041] (1) Preparation of crude polysaccharide solution of abalone viscera: thaw the frozen preserved abalone viscera naturally, mix 1 kg of abalone viscera with 3 kg of water, beat with a double-channel beater, and separate and remove the connective tissue in the abalone viscera , to obtain abalone viscera liquid; then mechanically separate the obtained abalone viscera liquid with a horizontal centrifuge to remove water-insoluble substances, and obtain a crude polysaccharide solution of abalone viscera;
[0042] (2) Enzymolysis: Use 0.1mol / L potassium hydroxide solution to adjust the pH value of the crude abalone viscera polysaccharide solution obtained in step (1) to 8, add 7% neutral protease by weight of the crude abalone viscera polysaccharide solution, and homogenize After 3 times, heat in a constant temperature wat...
Embodiment 3
[0053] A preparation method of abalone viscera polysaccharide powder for removing protein and heavy metal, which specifically comprises the following steps:
[0054] (1) Preparation of crude polysaccharide solution of abalone viscera: After thawing naturally frozen preserved abalone viscera, mix 1kg of abalone viscera with 2kg of water, and use a multi-functional beater for beating to separate and remove the connective tissue in the abalone viscera tissue to obtain abalone viscera liquid; then mechanically separate the obtained abalone viscera liquid by a fully automatic centrifuge to remove water-insoluble substances to obtain a crude polysaccharide solution of abalone viscera;
[0055] (2) Enzymolysis: Use 0.75wt% sodium hydroxide solution to adjust the pH value of the crude abalone viscera polysaccharide solution obtained in step (1) to 6.5, add 5% trypsin by weight of the crude abalone viscera polysaccharide solution, and homogenize twice Afterwards, heat in a constant tem...
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