Method for preparing abalone harslet polysaccharide powder for removing proteins and heavy metals

A technology for removing heavy metals and proteins, applied in the field of seafood research, can solve the problems of high protein content, achieve high purity, low requirements, and easy operation

Inactive Publication Date: 2016-07-06
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of abalone viscera polysaccharide powder for removing protein and heavy metals, which can effectively remove protein and heavy metals arsenic, lead, cadmium and nickel in polysaccharides, and solve the existing problems in the preparation process of polysaccharides. Problems such as heavy metals and high protein content, the polysaccharide powder produced by it has high purity, low technical cost, simple operation and easy popularization and application

Method used

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  • Method for preparing abalone harslet polysaccharide powder for removing proteins and heavy metals
  • Method for preparing abalone harslet polysaccharide powder for removing proteins and heavy metals
  • Method for preparing abalone harslet polysaccharide powder for removing proteins and heavy metals

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Embodiment 1

[0027] A preparation method of abalone viscera polysaccharide powder for removing protein and heavy metal, which specifically comprises the following steps:

[0028] (1) Preparation of crude polysaccharide solution of abalone viscera: thaw frozen abalone viscera naturally, mix 1 kg of abalone viscera with 1 kg of water, beat with a small single-channel beater, and separate and remove the connective tissue in the abalone viscera tissue to obtain abalone viscera fluid; then mechanically separate the obtained abalone viscera fluid to remove water-insoluble substances to obtain crude abalone viscera polysaccharide solution;

[0029] (2) Enzymolysis: Use 0.5 mol / L sodium hydroxide solution to adjust the pH value of the crude abalone viscera polysaccharide solution obtained in step (1) to 5, add 3% papain by weight of the crude abalone viscera polysaccharide solution, and homogenize After 2 times, heat in a constant temperature water bath at 45°C for 2 hours to obtain an enzymatic h...

Embodiment 2

[0040]A preparation method of abalone viscera polysaccharide powder for removing protein and heavy metal, which specifically comprises the following steps:

[0041] (1) Preparation of crude polysaccharide solution of abalone viscera: thaw the frozen preserved abalone viscera naturally, mix 1 kg of abalone viscera with 3 kg of water, beat with a double-channel beater, and separate and remove the connective tissue in the abalone viscera , to obtain abalone viscera liquid; then mechanically separate the obtained abalone viscera liquid with a horizontal centrifuge to remove water-insoluble substances, and obtain a crude polysaccharide solution of abalone viscera;

[0042] (2) Enzymolysis: Use 0.1mol / L potassium hydroxide solution to adjust the pH value of the crude abalone viscera polysaccharide solution obtained in step (1) to 8, add 7% neutral protease by weight of the crude abalone viscera polysaccharide solution, and homogenize After 3 times, heat in a constant temperature wat...

Embodiment 3

[0053] A preparation method of abalone viscera polysaccharide powder for removing protein and heavy metal, which specifically comprises the following steps:

[0054] (1) Preparation of crude polysaccharide solution of abalone viscera: After thawing naturally frozen preserved abalone viscera, mix 1kg of abalone viscera with 2kg of water, and use a multi-functional beater for beating to separate and remove the connective tissue in the abalone viscera tissue to obtain abalone viscera liquid; then mechanically separate the obtained abalone viscera liquid by a fully automatic centrifuge to remove water-insoluble substances to obtain a crude polysaccharide solution of abalone viscera;

[0055] (2) Enzymolysis: Use 0.75wt% sodium hydroxide solution to adjust the pH value of the crude abalone viscera polysaccharide solution obtained in step (1) to 6.5, add 5% trypsin by weight of the crude abalone viscera polysaccharide solution, and homogenize twice Afterwards, heat in a constant tem...

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Abstract

The invention discloses a method for preparing abalone harslet polysaccharide powder for removing proteins and heavy metals. The method comprises the following steps: preparing an abalone harslet polysaccharide crude solution, performing enzymolysis, performing low-temperature treatment, performing plate and frame filtration, performing ethanol treatment, performing resin adsorption and performing vacuum freeze drying. According to the method disclosed by the invention, the aim of removing proteins and heavy metals can be achieved during preparation of the abalone harslet polysaccharide, the protein removal rate is more than or equal to 90 percent, the removal rates of heavy metals such as arsenic, cadmium, lead and nickel are respectively more than or equal to 94 percent, more than or equal to 98 percent, more than or equal to 88 percent and more than or equal to 90 percent, the finally obtained abalone harslet polysaccharide powder has high purity, protein content of less than or equal to 10 percent and moisture content of less than or equal to 5 percent, and the safety of the product can be fully guaranteed. In addition, temperature in the whole preparation process does not exceed 80 DEG C, the activity of the polysaccharide can be remained to the greatest degree, and the method is simple, easy to operate, low in equipment requirement, low in cost and suitable for industrial application.

Description

technical field [0001] The invention belongs to the technical field of seafood research, and in particular relates to a method for preparing abalone viscera polysaccharide powder for removing protein and heavy metals. Background technique [0002] my country is rich in marine resources and has excellent conditions for aquaculture. It is the largest abalone-raising country in the world. Abalone’s stomach and feet are rich in protein, calcium, iron, vitamin A and other nutrients, and have the effects of destroying cancer cells, balancing blood pressure, nourishing yin and clearing heat, and improving eyesight. Favored by consumers at home and abroad, the market demand is also increasing day by day. [0003] Under normal circumstances, abalone is processed and eaten mainly with gastropods, and the abalone viscera, which accounts for 1 / 3 of the dry weight of abalone, is directly discarded, seriously polluting the environment. Studies have shown that abalone organs and tissues ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 贾真陈锦权方婷李长城
Owner FUJIAN AGRI & FORESTRY UNIV
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