Xinjiang juniper total flavonoids extract and its preparation method and application

The technology of total flavonoids and extracts is applied in the field of total flavonoids extract of juniper juniper and its preparation field, which can solve the problems of affecting the regeneration of macroporous adsorption resin, affecting the quality of total flavonoids extract, and unclean removal of fat-soluble components, and achieving Good anti-inflammatory and analgesic effect, easy regeneration, no heavy metal residue effect

Active Publication Date: 2019-10-11
XINJIANG INST OF MATERIA MEDICA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention provides an extract of total flavonoids from Sinica cypress and its preparation method and application, which overcomes the deficiencies in the prior art and can effectively solve the problems of low total flavonoid content and easy extraction of total flavonoids in existing methods for extracting total flavonoids from Sinica cypress. Hydrolysis of flavonoid glycosides into aglycones, cumbersome operation and the introduction of heavy metals, fat-soluble components can not be removed cleanly, affecting the quality of total flavonoid extracts, and also affecting the regeneration of macroporous adsorption resins

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025]Example 1, the total flavonoids extract of Sinica juniper is obtained according to the following preparation method: the first step, the branches and leaves of Sinica juniper are pulverized and dried, and the mixed medicinal material is obtained after drying, the mixed medicinal material is degreased with petroleum ether, and after the degreasing of petroleum ether Add ethanol solution to the medicinal materials, boil and then reflux extraction for 1 to 3 times. After extraction, the extracts are combined and concentrated to obtain a primary extract; in the second step, the primary extract and silica gel are mixed and dried. The packed silica gel packing chromatographic column is degreased for the second time with a mixed solution of petroleum ether and ethyl acetate, and then eluted with ethanol solution to collect the ethanol eluate. After the ethanol eluate is concentrated, the second leaching paste; the third step is to dilute the secondary extract with deionized wate...

Embodiment 2

[0026] Example 2, as an optimization of the above example, in the first step, the amount of ethanol solution added during each reflux extraction is 6 times to 20 times the mass of the mixed medicinal materials; or / and, each extraction time is 1h to 2h , the ethanol solution is 50% to 70% ethanol aqueous solution by volume percentage during each reflux extraction.

Embodiment 3

[0027] Example 3, as an optimization of the above example, in the first step, after extraction, the extracts were combined and concentrated until the relative density at 25° C. was 1.05 to 1.20 to obtain a primary extract.

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PUM

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Abstract

The invention relates to the technical field of juniperus sabina, and in particular relates to a juniperus sabina total flavonoid extract as well as a preparation method and an application thereof. The juniperus sabina total flavonoid extract is obtained according to the following method: crushing juniperus sabina branches and leaves, performing degreasing after drying, performing reflux extraction after degreasing by using an ethanol solution, performing concentration after extraction, removing impurities by virtue of a silica gel column, and performing enrichment and purification by virtue of macroporous resin to obtain the juniperus sabina total flavonoid extract provided by the invention. The method, in comparison with a traditional method, has the following advantages that (1) the use of acid and lead salts is avoided, the components are stable and cannot be hydrolyzed easily, and no heavy metal is remained; (2) after removing oil-soluble impurities by using silica gel, the pollution of the macroporous resin can be effectively reduced, and the macroporous resin is easy to regenerate; and (3) the enrichment and purification of flavonoid components in juniperus sabina can be realized in a closed environment, the total flavonoid content is high, the process method is simple to operate, is low in cost and is pollution-free, and meanwhile, the juniperus sabina total flavonoid extract provided by the invention has a relatively good effect of resisting inflammation and easing pain, and can be used for treating arthritis diseases.

Description

technical field [0001] The invention relates to the technical field of Xinjiang Junbai, and relates to an extract of Xinjiang Junbai total flavonoids and a preparation method and application thereof. Background technique [0002] Xinjiang juniper Juniperus sabina , also known as sandy cypress, fork juniper and stinky cypress, etc., is an evergreen creeping shrub of the genus Juniper, which is widely distributed in the arid barren hills and sandy lands of Xinjiang, Gansu, Inner Mongolia and northern Shaanxi in my country. Because of its ability to resist wind erosion, maintain water and soil, fix sand and protect slopes, and improve the environment, this plant has begun to be cultivated on a large scale in the above-mentioned areas. Xinjiang juniper branches and leaves are commonly used in the treatment of rheumatoid arthritis, rheumatoid arthritis and other diseases in my country, and are recorded in medical literature such as "Uygur Medicine Records", "Xinjiang Chinese Her...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/14A61P19/02A61P29/00
Inventor 赵军徐芳李晨阳陈燕李倩奥斯曼江·麦提图尔荪
Owner XINJIANG INST OF MATERIA MEDICA
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