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A kind of active and stable SOD skin care composition and preparation method thereof

A skin care composition, a stable technology, applied in the field of hygiene products, can solve the problems of easy deactivation of SOD, and achieve the effect of increased release, simple preparation method, rapid and effective release

Active Publication Date: 2019-05-14
广州微肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] SOD is an effective scavenger for superoxide anion radicals, but the existing SOD is easily inactivated

Method used

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  • A kind of active and stable SOD skin care composition and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] (1) Select fresh aloe vera leaves and garlic leaves with a mass ratio of 1:0.1, remove surface impurities, cut into small pieces, add 0.06mol / L phosphate buffer solution with a pH value of 7.8, and treat with 250W ultrasonic wave for 3min , stirred to obtain a uniform solution, and after centrifugation, the supernatant was taken to obtain a crude enzyme filtrate of 2.3 mg / ml.

[0028] (2) In parts by weight, heat 50 parts of the crude enzyme filtrate to 30°C, add 1 part of mannitol and 0.5 part of sucrose, stir and react for 20min, and after rapid cooling, at 30°C and a pressure of 1.5MPa, After ultrafiltration membrane filtration, the modified SOD enzyme solution with a permeability of 85% is obtained.

[0029] (3) After mixing the moisturizing agent silk peptide, the skin care agent lanolin, the preservative tea polyphenol, and the dispersing agent soybean protein surfactant, add the modified SOD enzyme liquid sterilized by high temperature, and stir evenly to obtain ...

Embodiment 2

[0031] (1) Select fresh aloe vera leaves and garlic leaves with a mass ratio of 1:10, remove surface impurities, cut into small pieces, add 0.08mol / L of phosphate buffer solution with a pH value of 7.8, and 300W ultrasonic treatment for 5min , stirred to obtain a uniform solution, and after centrifugation, the supernatant was taken to obtain a crude enzyme filtrate of 3.4 mg / ml.

[0032] (2) In parts by weight, heat 50 parts of the crude enzyme filtrate to 35°C, add 2 parts of mannitol and 2 parts of sucrose, stir and react for 30min, and after rapid cooling, at 30°C and a pressure of 1.5MPa, After ultrafiltration membrane filtration, the modified SOD enzyme solution with a permeability of 92% is obtained.

[0033] (3) After mixing the moisturizing agent silk peptide, the skin care agent lanolin, the preservative tea polyphenol, and the dispersing agent soybean protein surfactant, add the modified SOD enzyme liquid sterilized by high temperature, and stir evenly to obtain the ...

Embodiment 3

[0035] (1) Select fresh aloe vera leaves and garlic leaves with a mass ratio of 1:5, remove surface impurities, cut into small pieces, add 0.07mol / L phosphate buffer solution with a pH value of 7.8, and treat with 250W ultrasonic wave for 4min , stirred to obtain a uniform solution, and after centrifugation, the supernatant was taken to obtain a crude enzyme filtrate of 2.7 mg / ml.

[0036] (2) In parts by weight, heat 50 parts of the crude enzyme filtrate to 30-35°C, add 1.5 parts of mannitol and 1 part of sucrose, stir and react for 25min, and after cooling rapidly, under the pressure of 30°C and 1.5MPa , after being filtered by an ultrafiltration membrane, a modified SOD enzyme liquid with a permeability of 90% is obtained.

[0037] (3) After mixing the moisturizing agent silk peptide, the skin care agent lanolin, the preservative tea polyphenol, and the dispersing agent soybean protein surfactant, add the modified SOD enzyme liquid sterilized by high temperature, and stir e...

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Abstract

The invention provides an SOD skin-caring composition with stable activity and a preparing method thereof.The preparing method includes the specific steps that 1, fresh aloe leaves and fresh garlic leaves are selected, chipped after surface impurity removing and added into phosphate buffer for ultrasonic treatment, the mixture is stirred to obtain an even solution, supernatant is taken after centrifuging separating, and crude enzyme filter liquor is obtained; 2, the crude enzyme filter liquor prepared in the step 1 is heated to be at 30 DEG C-35 DEG C, mannitol and cane sugar are added, the mixture is stirred and reacted for 20 min-30 min, rapidly cooled and filtered through a ultrafiltration membrane, and modified SOD enzyme liquor is obtained; 3, the modified SOD enzyme liquor prepared in the step 2 is sterilized at high temperature and added into a natural skin-caring composition, the mixture is stirred to be even, and the SOD skin-caring composition with stable activity is obtained.The preparing method of the SOD skin-caring composition with stable activity is easy and convenient, the activity of SOD prepared by combining the physical modification technique and the chemical modification technique is stable, effective ingredients can be provided for a long time, and the long-time skin caring aim can be achieved.

Description

technical field [0001] The invention belongs to the technical field of sanitary products, and in particular relates to an active and stable SOD skin care composition and a preparation method thereof. Background technique [0002] With the continuous improvement of living standards, people's requirements for cosmetics are becoming more and more high-level. People are no longer satisfied with simple skin care products, but also need skin care products with special functions, such as removing spots, preventing wrinkles, and maintaining skin. Elasticity etc. [0003] Superoxide dismutase (SOD) belongs to metalloenzyme, which mostly exists in animals, plants, algae and other prokaryotic organisms. The amino acid sequences of purified enzymes extracted from different sources are slightly different, so the properties are also different. The properties of SOD depend not only on the protein part, but also on the metal ion at the active site of the binder. According to the type of me...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K8/9794A61K8/66A61K8/64A61K8/92A61K8/34A61Q19/08A61Q19/00
CPCA61K8/347A61K8/64A61K8/645A61K8/66A61K8/925A61K8/97A61Q19/00A61Q19/08
Inventor 胡海威丁靓闫永平郑辉严辉
Owner 广州微肽生物科技有限公司
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