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Preparation and application of aminoglycoside antibiotic complex degrading bacteria

A technology of aminoglycosides and antibiotic bacteria residues, applied in the direction of microorganism-based methods, bacteria, microorganisms, etc., can solve problems such as damage to vestibular and auditory epithelial cells, spread, and aggravate the formation of multidrug-resistant bacteria

Active Publication Date: 2016-07-20
FUJIAN NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Antibiotic-containing wastewater and waste residues in the antibiotic production process may cause potential environmental problems, cause the spread of antibiotic resistance genes, and aggravate the formation of multidrug-resistant bacteria
And aminoglycoside antibiotics have strong ototoxicity and nephrotoxicity, will damage the vestibular and auditory epithelial cells, endanger human health, and the environmental hazards are even more serious

Method used

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  • Preparation and application of aminoglycoside antibiotic complex degrading bacteria
  • Preparation and application of aminoglycoside antibiotic complex degrading bacteria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] 1. Cultivation of degrading bacteria seed solution

[0037] The aminoglycoside antibiotic-degrading bacteria Aquamicrobiumsp.FN-A3 strain and ParacoccuskondratievaeFN-A2 strain were respectively connected to fresh LB slant culture medium, and incubated at a constant temperature of 37°C and 180r / min for 16h to prepare the aminoglycoside antibiotic Aquamicrobiumsp.FN- A3 degrading bacteria seed liquid and ParacoccuskondratievaeFN-A2 degrading bacteria seed liquid.

[0038] 2. Preparation of composite degradation bacteria

[0039] Mix aminoglycoside antibiotics Aquamicrobiumsp.FN-A3 and ParacoccuskondratievaeFN-A2 degrading bacteria seed solution according to the volume ratio of 3:5, and mix them with cornstarch according to the weight ratio of 1:1 to prepare composite degrading bacteria. Air dry and set aside.

[0040] 3. Application

[0041] 1) Degradation of aminoglycoside antibiotics remaining in kanamycin wastewater by composite degrading bacteria

[0042] Inocula...

Embodiment 2

[0050] The seed solution cultivation of degrading bacteria, the preparation and activation of composite degrading bacteria are the same as in Example 1.

[0051] 1. Degradation of residual streptomycin in sewage by composite degrading bacteria

[0052] Add 500uL compound degrading bacteria seed solution into 50ml sewage containing 1000mg / L streptomycin, and culture at 37°C and 180r / min constant temperature shaking for 20h. After testing, streptomycin is basically degraded, and the degradation rate reaches 92.8%.

[0053] 2. Degradation of residual streptomycin in bacterial residue by composite degrading bacteria

[0054] Take 1000g of fresh streptomycin residue, add 100mL of compound degrading bacteria seed solution, mix well, control the total water content at 65% after mixing, and ferment for 60h to obtain bacteria residue without antibiotic residue. After testing, the residual streptomycin in the fermentation product of the bacteria residue has been completely degraded, an...

Embodiment 3

[0057] The seed solution cultivation of degrading bacteria, the preparation and activation of composite degrading bacteria are the same as in Example 1.

[0058] 1. Degradation of residual gentamicin in sewage by composite degrading bacteria

[0059] Add 500uL compound degrading bacteria seed liquid into 50ml sewage containing 3000mg / L gentamicin, and culture at 37°C and 180r / min constant temperature shaking for 24h. After testing, the degradation rate of gentamicin reaches 97%.

[0060] 2. Degradation of gentamicin in soil by compound degrading bacteria

[0061] Take 1000g of soil containing gentamicin 800mg / L, add 600mL of compound degrading bacteria seed liquid, mix well, control the total water content at 55% after mixing, and ferment for 90h. After testing, the residual gentamicin in the fermentation product of the bacteria residue has been basically degraded, with a degradation rate of 88.6%. During the fermentation process, flip twice a day for natural ventilation, th...

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Abstract

The invention relates to preparation and application of aminoglycoside antibiotic complex degrading bacteria. The scheme is that a preparation method comprises the following steps: respectively inoculating an Aquamicrobium Sp. FN-A3 strain and a Paracoccus kondratievae FN-A2 strain in LB culture solution for culture to respectively obtain degrading bacteria seed solution; mixing the degrading bacteria seed solution according to a volume ratio of 3:1, performing uniform mixing, then mixing the seed solution and corn starch according to a weight ratio of 1:(1-3), and performing air-drying to obtain the aminoglycoside antibiotic complex degrading bacteria. The invention adopts a modern microbiological technique to perform hazard-frees fermentation treatment to antibiotic bacteria residues or biological remediation to antibiotic polluted water bodies or soil to remove potential environmental and health hazards, provides a new approach for related enterprises to safely and efficiently treat bacteria residues and wastewater associated with production processes, and facilitates the resource application of the bacteria residues.

Description

technical field [0001] The invention relates to the field of antibiotic fermentation and environmental protection, in particular to the preparation and application of an aminoglycoside antibiotic compound degrading bacterium. Background technique [0002] Aminoglycoside antibiotics are a class of antibiotics linked by amino sugars and aminocycloalcohols through oxygen bridges, mainly produced by actinomycetes. In 1943, streptomycin was first discovered from Streptomyces griseus, becoming the first clinical antibiotic that can effectively treat tuberculosis. Subsequently, many such antibiotics were discovered and used in clinical treatment, such as neomycin (neomycin, 1949), kanamycin (kanamycin, 1957), spectinomycin (spectinomycin, 1961), gentamicin (gentamicin, 1963 ), tobramycin (tobramycin, 1967); and the research on the second-generation aminoglycoside antibiotics mining and semi-synthetic derivatives also entered a climax, such as sisomicin (sisomicin, 1970), dibekacin...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34B09C1/10C12R1/01C02F101/38
CPCB09C1/10C02F3/34C02F2101/38C12N1/02C12N1/20
Inventor 王明兹方森海吕月霞吴钦缘陈必链张会
Owner FUJIAN NORMAL UNIV