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A maintenance solution for preserving pig whole blood and its application method

A technology of maintenance solution and pig whole blood, which is applied in the field of maintenance solution, can solve the problems of large histogram difference, high cost of raw materials, short validity period, etc., and achieve the effect of reducing matrix effect, prolonging storage time and promoting viscosity

Active Publication Date: 2018-10-09
SICHUAN XINCHENG BIOLOGICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is: the existing blood cell analysis quality control products that can replace human blood have high raw material costs, large differences in histograms, and short validity periods. A maintenance solution for preserving pig whole blood with a long shelf life of blood raw materials

Method used

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  • A maintenance solution for preserving pig whole blood and its application method
  • A maintenance solution for preserving pig whole blood and its application method
  • A maintenance solution for preserving pig whole blood and its application method

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The present invention is a maintenance solution for preserving pig whole blood. The configuration and application method of the maintenance solution are as follows:

[0031] 1) Anticoagulant preparation: In water, add 2.8g of citric acid, 19g of sodium citrate, EDTA-Na 2 50g, then dilute to 1L.

[0032] 2) Preparation of curing agent: Add 18g of disodium hydrogen phosphate, 4.5g of potassium dihydrogen phosphate, 40ml of formaldehyde, 2g of adenine, 30g of sucrose, 10g of sodium chloride, and 8g of aspirin into water, then dilute to 1L.

[0033] 3) Antibacterial agent preparation: Add 200 million units of penicillin to the water, and then adjust the volume to 1L.

[0034] 4) Collect 500ml of healthy pig blood, add 25ml of anticoagulant, 15ml of curing agent, and 5ml of antibacterial agent, and mix the ingredients in time.

[0035] 5) Perform a performance check on the obtained pig blood mixture, and subpackage the pig blood mixture. The subpackage requires thorough m...

Embodiment 2

[0043]The difference between this embodiment and embodiment 1 is that the addition amount of each component is different, and the specific settings are as follows:

[0044] In the anticoagulant preparation, the addition amount of each substance is: citric acid 1.5g, sodium citrate 12g, EDTA-Na 2 40g, then dilute to 1L. In the preparation of the curing agent, the amount of each substance added is: 15g of disodium hydrogen phosphate, 3g of potassium dihydrogen phosphate, 30ml of formaldehyde, 1g of adenine, 10g of sucrose, 5g of sodium chloride, 5g of aspirin, and then adjust the volume to 1L. In the preparation of the antibacterial agent, the addition amount of each substance is: 150 million units of penicillin, and then set the volume to 1L.

[0045] In this embodiment, for every 500ml of healthy pig blood, the amount of anticoagulant added is 35ml, the amount of curing agent added is 25ml, and the amount of bacteriostatic agent added is 10ml.

[0046] Using the same method...

Embodiment 3

[0053] The difference between this embodiment and embodiment 1 is that the addition amount of each component is different, and the specific settings are as follows:

[0054] In this anticoagulant preparation, the addition amount of each substance is: citric acid 1g, sodium citrate 5g, EDTA-Na 2 15g, then dilute to 1L. In the preparation of the curing agent, the amount of each substance added is: 2g of disodium hydrogen phosphate, 0.5g of potassium dihydrogen phosphate, 10ml of formaldehyde, 0.2g of adenine, 5g of sucrose, 1g of sodium chloride, 1g of aspirin, and then set the volume to 1L. Can. In the preparation of the antibacterial agent, the addition amount of each substance is: 100 million units of penicillin, and then set the volume to 1L.

[0055] In this embodiment, for every 500ml of healthy pig blood, the amount of anticoagulant added is 50ml, the amount of curing agent added is 45ml, and the amount of bacteriostatic agent added is 20ml.

[0056] Using the same me...

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Abstract

The invention discloses a preservative solution for storing pig whole blood and an application method thereof.The problems that raw materials capable of replacing human blood hemocyte analysis quality control products are high in cost, large in histogram difference and short in efficacy time are solved.The preservative solution comprises anticoagulant, a curing agent and a bacteriostatic agent.The anticoagulant comprises citric acid, sodium citrate and EDTA-Na2.The curing agent comprises sodium hydrogen phosphate, monopotassium phosphate, methyl aldehyde, adenine, cane sugar, sodium chloride and aspirin.The bacteriostatic agent comprises penicillin.The preservative solution has the advantages that cost is low, the storage life of blood can be effectively prolonged to 10 months or above, the difference between bottles can be reduced, the variable coefficient CV of the difference between the bottles is made to be smaller than or equal to 5%, the constant value is accurate, stability is high within the storage life, and the variable coefficient CV of stability is smaller than or equal to 7%.

Description

technical field [0001] The invention relates to a maintenance solution, in particular to a maintenance solution for preserving pig whole blood, and provides an application method of the maintenance solution. Background technique [0002] At present, most of the blood cell analysis quality control products used in my country are imported brands, such as Beckman Coulter and Abbott in the United States, SYSMEX in Japan, and Bayer in Germany. Imported blood cell analysis quality control products need to be used in conjunction with the corresponding blood cell analyzers, and instruments of different brands cannot be used interchangeably; moreover, the stability time of imported blood cell analysis quality control products is relatively short, only 3 months; due to the lack of localization and blood product production Difficulty in customs clearance and other factors lead to long ordering cycles, coupled with transportation time, users often get products that are close to or even ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02G01N33/96
CPCA01N1/021G01N33/96
Inventor 杨溢张良黄涛
Owner SICHUAN XINCHENG BIOLOGICAL CO LTD