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Method for co-preparing ovalbumin and ovotransferrin

A technology for ovalbumin and ovotransferrin, applied in the field of co-preparing ovalbumin and ovotransferrin, can solve the problems of desalting or small scale, need expensive equipment, limited separation scale, etc., and achieve short production cycle and easy large-scale production. The effect of large-scale industrial production and simple and easy process operation

Active Publication Date: 2016-07-27
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, ammonium sulfate, sodium sulfate and sodium chloride are commonly used for salting out to prepare a large amount of ovalbumin. However, the product contains high salt and requires multiple desalination to obtain ovalbumin of higher purity.
In addition, ultrafiltration technology can also prepare ovalbumin, but it requires expensive equipment and the separation scale is relatively limited
Ovotransferrin can be separated and extracted by salting out method, ion exchange method or immobilized metal chelation affinity chromatography, but there are also problems of desalting or small scale

Method used

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  • Method for co-preparing ovalbumin and ovotransferrin
  • Method for co-preparing ovalbumin and ovotransferrin
  • Method for co-preparing ovalbumin and ovotransferrin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Take 100ml of fresh egg white, add 200ml of fresh deionized water, adjust the pH to about 5.0, stir evenly for 1 hour, and centrifuge at 8000rpm for 30min to remove the precipitate. Add polyethylene glycol 20000 to the supernatant A obtained by centrifugation until its mass percentage concentration is 15%, stir for 1 hour, centrifuge at 8000rpm for 30min, collect the precipitate B, and dissolve the precipitate with 50ml of 20mM Tris solution (pH8.0) , stirred for 1 hour to fully dissolve the precipitate, then added polyethylene glycol 200 solution to the resulting solution to a mass percent concentration of 8%, stirred for another 1 hour, centrifuged at 8000rpm for 30min, and collected supernatant C and precipitate D. The obtained supernatant C was detected by sodium dodecylsulfonate-polyacrylamide gel electrophoresis and showed that it contained ovalbumin with a purity of 99.7%, and the total yield was 61.5%; the obtained precipitate D was subjected to sodium dodecylsul...

Embodiment 2

[0025] Take 100ml of fresh egg white, add 200ml of fresh deionized water, adjust the pH to about 5.0, stir evenly for 1 hour, and centrifuge at 8000rpm for 30min to remove the precipitate. Add polyethylene glycol 200 to the supernatant A obtained by centrifugation until its mass percentage concentration is 30%, stir for 1 hour, centrifuge at 8000rpm for 30min, collect the precipitate B, and dissolve the precipitate with 50ml of 20mM Tris solution (pH8.0) , stirred for 1 hour to fully dissolve the precipitate, then added polyethylene glycol 200 solution to the resulting solution to a mass percent concentration of 15%, stirred for another 1 hour, centrifuged at 8000rpm for 30min, and collected supernatant C and precipitate D. The obtained supernatant C was detected by sodium dodecylsulfonate-polyacrylamide gel electrophoresis and showed that it contained ovalbumin with a purity of 91.6%, and the total yield was 72.3%; the obtained precipitate D was subjected to sodium dodecylsulf...

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Abstract

The invention discloses a method for co-preparing ovalbumin and ovotransferrin, and relates to the technical fields of biologics, medicines and good industry. The method comprises the following steps: collecting egg white at the room temperature, diluting the egg white with fresh deionized water according to a volume ratio of 1:2, adjusting the pH value to be about 5.0, uniformly stirring, and centrifuging; adding polyethylene glycol of which the molecular weight is between 200 to 20000 into the supernate A obtained after centrifugation, uniformly stirring, centrifuging, and collecting precipitate B; and dissolving the precipitate B, adding polyethylene glycol of which the molecular weight is between 200 to 20000, centrifuging, and collecting supernate C and precipitate D, wherein the supernate C contains high-purity ovalbumin; and the precipitate D contains ovotransferrin with relatively high purity. The method is simple, convenient and rapid, low in cost, mild and safe in condition, and applicable to large-scale industrial production of ovalbumin and ovotransferrin.

Description

technical field [0001] The invention relates to the technical fields of biology, medicine and food industry, and more specifically relates to a process for co-preparing ovalbumin and ovotransferrin. Background technique [0002] Eggs are composed of three parts: eggshell, egg white and egg yolk. Egg white accounts for about 60% to 63% of the total weight and is rich in active proteins. Of these proteins, 54% are ovalbumin and 12% are ovotransferrin ( Ovotransferrin). Ovalbumin is a high-quality protein that can provide 8 kinds of amino acids necessary for the human body; it can form stable complexes with iron (III) ions to prepare new biopolymer carrier iron supplements; prepare ovalbumin polypeptides, ovalbumin powder and nutrition Oral liquid, enhance the body's immunity and inhibit the activity of angiotensin-converting enzyme. Ovotransferrin has antibacterial, antiviral and antifungal activities similar to human transferrin and lactoferrin, and can be used as a good im...

Claims

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Application Information

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IPC IPC(8): C07K14/77C07K14/79C07K1/30
CPCC07K14/77C07K14/79
Inventor 闻崇炜葛藤赵烨清周慧莲石莉
Owner JIANGSU UNIV
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