SNP (single nucleotide polymorphism) detection kit for nitroglycerin drug-sensitive type relevant gene ALDH2 and use method thereof
A detection kit and nitroglycerin technology, applied in the field of medical detection, can solve problems such as long operation time, unsuitability for rapid clinical detection, and difficulty in clinical promotion, and achieve the effect of easy judgment
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[0040] The present invention will be described in further detail below in conjunction with the embodiments given in the accompanying drawings.
[0041] refer to figure 1 As shown, the SNP detection kit of a nitroglycerin drug-sensitive related gene ALDH2 in this embodiment includes the following components by volume:
[0042]
[0043] The primers include:
[0044] Forward primer: ALDH2*2-F: 5'-CGGGAGTTGGGCGAGTA-3';
[0045] Reverse primer: ALDH2*2-R: 5'-TCCGAGCCACCAGCAGA-3';
[0046] The probes include:
[0047] ALDH2*2 wild-type probe: 5'-fluorophore-GTTTTCACTTCAGTGTATGCC-MGB-3';
[0048] ALDH2*2 mutant probe: 5'-fluorophore-GTTTTCACTTTAGTGTATGCC-MGB-3';
[0049] As a further improvement of the present invention, the premix is a 2×PCR enzyme reaction premix, and the 2×PCR enzyme reaction premix contains 0.1 units / μl of TaqDNA Polymerase (recombinant), 4 mmol / L of MgCl 2 , 0.5mmol / L of dNTPs.
[0050] As an improved specific implementation, the solvent is double di...
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