SNP (single nucleotide polymorphism) detection kit for nitroglycerin drug-sensitive type relevant gene ALDH2 and use method thereof

A detection kit and nitroglycerin technology, applied in the field of medical detection, can solve problems such as long operation time, unsuitability for rapid clinical detection, and difficulty in clinical promotion, and achieve the effect of easy judgment

Inactive Publication Date: 2016-07-27
浙江中迪生物科技有限公司
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Problems solved by technology

[0004] At present, the most common detection method for gene polymorphism is sequencing method, which is suitable for high-throughput multi-site detection, but it is time-consuming and low-sensitivity, so it is not suitable for rapid clinical detection; high-res

Method used

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  • SNP (single nucleotide polymorphism) detection kit for nitroglycerin drug-sensitive type relevant gene ALDH2 and use method thereof
  • SNP (single nucleotide polymorphism) detection kit for nitroglycerin drug-sensitive type relevant gene ALDH2 and use method thereof
  • SNP (single nucleotide polymorphism) detection kit for nitroglycerin drug-sensitive type relevant gene ALDH2 and use method thereof

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Embodiment Construction

[0040] The present invention will be described in further detail below in conjunction with the embodiments given in the accompanying drawings.

[0041] refer to figure 1 As shown, the SNP detection kit of a nitroglycerin drug-sensitive related gene ALDH2 in this embodiment includes the following components by volume:

[0042]

[0043] The primers include:

[0044] Forward primer: ALDH2*2-F: 5'-CGGGAGTTGGGCGAGTA-3';

[0045] Reverse primer: ALDH2*2-R: 5'-TCCGAGCCACCAGCAGA-3';

[0046] The probes include:

[0047] ALDH2*2 wild-type probe: 5'-fluorophore-GTTTTCACTTCAGTGTATGCC-MGB-3';

[0048] ALDH2*2 mutant probe: 5'-fluorophore-GTTTTCACTTTAGTGTATGCC-MGB-3';

[0049] As a further improvement of the present invention, the premix is ​​a 2×PCR enzyme reaction premix, and the 2×PCR enzyme reaction premix contains 0.1 units / μl of TaqDNA Polymerase (recombinant), 4 mmol / L of MgCl 2 , 0.5mmol / L of dNTPs.

[0050] As an improved specific implementation, the solvent is double di...

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Abstract

The invention discloses an SNP (single nucleotide polymorphism) detection kit for a nitroglycerin drug-sensitive type relevant gene ALDH2 and a use method thereof. The human genome DNA (deoxyribonucleic acid) is used as a template; a corresponding specific primer and a fluorescence probe for detecting the ALDH2*2 type polymorphism are respectively added for forming a real-time fluorescence quantitative PCR (polymerase chain reaction) system; through real time PCR reaction, technicians in the field determine the gene type of the ALDH2 through data collected by a fluorescence quantitative PCR instrument. The SNP detection kit has the advantages of high speed, sensitivity, easy judgment and the like, and is suitable for clinic detection and popularization.

Description

technical field [0001] The invention relates to the field of medical detection, and more specifically relates to a SNP detection kit for nitroglycerin drug-sensitive related gene ALDH2 and a use method thereof. Background technique [0002] Nitroglycerin (GTN, glyceryltrinitrate, nitroglycerin) has been used to treat angina pectoris and heart failure for 130 years. Sublingual administration of nitroglycerin tablets is the routine first-choice prescription for anti-angina pectoris acute attack of coronary heart disease, but its clinical effectiveness often varies from person to person. varies. Some people saved their lives in time because of it, but after some people took it, the effect was not very obvious. In the past, the domestic and foreign medical circles have always believed that the poor curative effect of nitroglycerin on some patients is caused by its "drug resistance", but the research results prove that this is not the case. Recent studies have shown that gene m...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6858C12Q1/6883C12Q2600/106C12Q2600/156C12Q2531/113C12Q2563/107C12Q2561/113
Inventor 邢洋苏彩环林芳珍
Owner 浙江中迪生物科技有限公司
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