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Method for preparing nonreactive environment-friendly feed through synergistic fermentation of bacterium enzymes

A collaborative fermentation and environmental protection technology, applied in animal feed, animal feed, applications, etc., can solve the problems of difficult operation and many production operations, and achieve the effect of safe and controllable fermentation, good palatability, and increased value

Pending Publication Date: 2016-08-03
武汉泽元科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent application number 201210157981.1 "Detoxification agent for cotton meal and rapeseed meal and its preparation method and preparation method of microbial feed made with the detoxification agent" discloses the use of cotton meal or rapeseed meal, corn flour, The bran is inoculated with phosphate-dissolving bacteria, bifidobacteria and Lactobacillus acidophilus complex bacteria activated in water added with glucose, the material is sealed for anaerobic fermentation after mixing and inoculation, and after 3-5 days of fermentation, it is air-dried to obtain a fermented product. This technical scheme Only one raw material of cotton meal or rapeseed meal is selected, and there are many production operations, and finally it needs to be dried in the air for actual large-scale production. The operation is more difficult

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: A kind of method for the preparation of non-antibiotic environment-friendly feed by bacterium-enzyme synergistic fermentation, comprises the following steps:

[0029] (1) Prepare the composite bacterial liquid required for fermentation;

[0030] The composite bacterium includes yeast, Bacillus subtilis, Bacillus lateralis and Lactobacillus plantarum, and the parts by weight of each component are: 23 parts of yeast, 22 parts of Bacillus subtilis, 11 parts of Bacillus lateralis, vegetable milk 38 parts of bacilli, the above composite bacteria were added to warm water at 40°C for 0.5 hours to make the number of bacteria 1.0×10 8 individual / ml composite bacteria solution;

[0031] (2) Prepare the compound enzyme liquid required for fermentation;

[0032] The compound enzyme includes acid protease and cellulase, and the parts by weight of each component are 20 parts of acid protease and 17 parts of cellulase. Add the above compound enzyme to warm water at 42°...

Embodiment 2

[0041] Embodiment 2: a kind of method for the preparation of antibiotic-free environment-friendly feed by bacterium-enzyme synergistic fermentation, comprises the following steps:

[0042] (1) Prepare the composite bacterial liquid required for fermentation;

[0043] The composite bacterium includes yeast, Bacillus subtilis, Bacillus lateralis and Lactobacillus plantarum, and the parts by weight of each component are respectively: 30 parts of yeast, 34 parts of Bacillus subtilis, 8 parts of Bacillus lateralis, vegetable milk 22 parts of bacilli, the above composite bacteria were added to warm water at 37°C for 1 hour to make the number of bacteria 0.7×10 7 individual / ml composite bacteria solution;

[0044] (2) Prepare the compound enzyme liquid required for fermentation;

[0045] The compound enzyme includes acid protease and cellulase, and the parts by weight of each component are 25 parts of acid protease and 15 parts of cellulase. Add the above compound enzyme to warm wa...

Embodiment 3

[0054] Embodiment 3: a kind of method for bacterial enzyme synergistic fermentation to prepare antibiotic-free environmental protection feed, comprises the following steps:

[0055] (1) Prepare the composite bacterial liquid required for fermentation;

[0056] The composite bacterium includes yeast, Bacillus subtilis, Bacillus lateralis and Lactobacillus plantarum, and the parts by weight of each component are respectively: 15 parts of yeast, 28 parts of Bacillus subtilis, 24 parts of Bacillus lateralis, vegetable milk 22 parts of bacilli, the above composite bacteria were added to warm water at 35°C for 1.5 hours to make the number of bacteria 0.5×10 7 individual / ml composite bacteria solution;

[0057] (2) Prepare the compound enzyme liquid required for fermentation;

[0058] The compound enzyme includes acid protease and cellulase, and the parts by weight of each component are 10 parts of acid protease and 20 parts of cellulase. Add the above compound enzyme to warm water...

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PUM

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Abstract

The invention relates to a method for preparing nonreactive environment-friendly feed through synergistic fermentation of bacterium enzymes and belongs to the technical field of solid-state fermentation feed.Bean pulp, rapeseed meal, cottonseed meal, bran and folium artemisiae argyi powder are mixed to serve as a fermentation substrate, the fermentation substrate is inoculated with fermentation liquor prepared from yeast, bacillus subtilis, bacillus laterosporus, lactobacillus plantarum, acid proteinase and cellulase, the fermentation substrate obtained after inoculation is put in packaging bags with respiration films, the packaging bags are sealed and put in a constant-temperature fermentation chamber and then stacked, and the nonreactive environment-friendly feed is prepared through constant-temperature fermentation.Low-value agricultural and sideline products and Chinese herbal medicine which are cheap and easy to obtain are treated through the bioengineering technology, the production cost is low, antinutritional factors in the fermentation substrate can be efficiently degraded, beneficial viable bacteria in fermentation materials are rich, nutrients are rich, the effect of improving the immunity of fed animals is achieved, the concentration of ammonia in a livestock and poultry breeding house is remarkably reduced, and the safe and environment-friendly feed is provided for nonreactive breeding.

Description

technical field [0001] The invention relates to the technical field of solid-state fermented feed, in particular to a method for preparing antibiotic-free and environment-friendly feed through synergistic fermentation with bacteria and enzymes. Background technique [0002] China has always been a big animal husbandry and feed production country in the world. In 2015, China's total feed production hit a new high of 200 million tons, ranking first in the world. At the same time, China is a large feed consumption country, and the animal husbandry and feed industries continue to grow rapidly. At present, high-quality protein feed raw materials such as soybean meal have become dependent on a large amount of imports to meet the supply of domestic feed production. At the same time, my country is a large traditional agricultural planting country. The annual output of cottonseed meal in my country can reach 13 million tons, and the output of other miscellaneous meal can reach 8 mill...

Claims

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Application Information

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IPC IPC(8): A23K10/18A23K10/30
Inventor 齐威
Owner 武汉泽元科技有限公司
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