Gold-nanoparticle fluorescence probe and preparation method and application thereof

Abstract

The invention provides a gold-nanoparticle fluorescence probe and a preparation method and application thereof. The preparation method comprises the steps that 1-10 parts of 3-5 mmol / L chloroauric acid aqueous solution is heated to 80 DEG C to 120 DEG C, 1-5 parts of 4-6 mmol / L 2-mercapto-4-methyl-5-thiazoleacetic acid aqueous solution is added to the chloroauric acid aqueous solution, continuous heating stirring is conducted for 5 min to 30 min, and the gold-nanoparticle fluorescence probe is obtained. The preparation method is simple in reaction condition, rapid and friendly to environment. 2-mercapto-4-methyl-5-thiazoleacetic acid serves as a reducing agent and a protective agent, 2-mercapto-4-methyl-5-thiazoleacetic acid itself has the reducing property, and addition of a frequently-used reducing agent sodium borohydride, surfactants and the like is avoided. The gold-nanoparticle fluorescence probe presents intense blue fluorescence on the fluorescence emission peak near 430 nm, the gold-nanoparticle fluorescence probe is good in stability when preserved at room temperature and can be used for high-sensitivity and high-selectivity detection of Fe<3+>, the detection process is simple, convenient and rapid, and the detection result is accurate.

Description

technical field [0001] The invention relates to a fluorescent probe, in particular to a water-soluble blue gold nano fluorescent probe and its preparation method and application. Background technique [0002] Iron is one of the most important components of hemoglobin. It participates in the transport of oxygen and is also essential to maintain the normal functions of other oxygen-carrying proteins such as ferrohemoglobin, myoglobin, and cytochrome. It is also a part of many enzymes involved in energy metabolism It is an important component of human body, which has a serious impact on human health, especially the blood system. Iron deficiency can lead to too few red blood cells and even anemia, and conversely, iron excess can also cause various diseases such as Parkinson's, Alzheimer's and cancer. At present, there are many traditional scientific methods for iron detection, such as atomic absorption spectrometry, inductively coupled plasma mass spectrometry, and gas chromato...

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Problems solved by technology

The reported method requires the use of the strong reducing agent NaBH 4 , surfactant (hexadecyltrimethylammonium bromide) or strong alkaline reagent NaOH, etc., and the preparation steps are cumbersome and require a long time

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