Gold-nanoparticle fluorescence probe and preparation method and application thereof
A fluorescent probe and gold nanotechnology, applied in the field of fluorescent probes, can solve the problems of complicated preparation steps and long time required, and achieve the effects of simple detection means, rapid reaction and simple reaction conditions.
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Embodiment 1
[0022] Heat 7mL 4mmol / L chloroauric acid aqueous solution to 100°C, then add 2mL 5mmol / L 2-mercapto-4-methyl-5-thiazole acetic acid aqueous solution to the above chloroauric acid aqueous solution and continue heating and stirring for 20min to obtain gold nanofluorescence probe. The fluorescence emission peak of the gold nanometer fluorescent probe is near 430nm, showing strong blue fluorescence, and the quantum yield is 4.5%.
Embodiment 2
[0024] Heat 10mL of 4mmol / L chloroauric acid aqueous solution to 120°C, then add 1mL of 5mmol / L 2-mercapto-4-methyl-5-thiazoleacetic acid aqueous solution into the above chloroauric acid aqueous solution and continue heating and stirring for 30min to obtain gold nanofluorescence probe. The fluorescence emission peak of the gold nanometer fluorescent probe is near 430nm, showing strong blue fluorescence, and the quantum yield is 3.5%.
Embodiment 3
[0026] Heat 1mL of 4mmol / L chloroauric acid aqueous solution to 110°C, then add 5mL of 5mmol / L 2-mercapto-4-methyl-5-thiazoleacetic acid aqueous solution into the above chloroauric acid aqueous solution and continue heating and stirring for 20min to obtain gold nanofluorescence probe. The fluorescence emission peak of the gold nanometer fluorescent probe is near 430nm, showing strong blue fluorescence, and the quantum yield is 2.7%.
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