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Gold-nanoparticle fluorescence probe and preparation method and application thereof

A fluorescent probe and gold nanotechnology, applied in the field of fluorescent probes, can solve the problems of complicated preparation steps and long time required, and achieve the effects of simple detection means, rapid reaction and simple reaction conditions.

Inactive Publication Date: 2016-08-03
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The reported method requires the use of the strong reducing agent NaBH 4 , surfactant (hexadecyltrimethylammonium bromide) or strong alkaline reagent NaOH, etc., and the preparation steps are cumbersome and require a long time

Method used

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  • Gold-nanoparticle fluorescence probe and preparation method and application thereof
  • Gold-nanoparticle fluorescence probe and preparation method and application thereof
  • Gold-nanoparticle fluorescence probe and preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Heat 7mL 4mmol / L chloroauric acid aqueous solution to 100°C, then add 2mL 5mmol / L 2-mercapto-4-methyl-5-thiazole acetic acid aqueous solution to the above chloroauric acid aqueous solution and continue heating and stirring for 20min to obtain gold nanofluorescence probe. The fluorescence emission peak of the gold nanometer fluorescent probe is near 430nm, showing strong blue fluorescence, and the quantum yield is 4.5%.

Embodiment 2

[0024] Heat 10mL of 4mmol / L chloroauric acid aqueous solution to 120°C, then add 1mL of 5mmol / L 2-mercapto-4-methyl-5-thiazoleacetic acid aqueous solution into the above chloroauric acid aqueous solution and continue heating and stirring for 30min to obtain gold nanofluorescence probe. The fluorescence emission peak of the gold nanometer fluorescent probe is near 430nm, showing strong blue fluorescence, and the quantum yield is 3.5%.

Embodiment 3

[0026] Heat 1mL of 4mmol / L chloroauric acid aqueous solution to 110°C, then add 5mL of 5mmol / L 2-mercapto-4-methyl-5-thiazoleacetic acid aqueous solution into the above chloroauric acid aqueous solution and continue heating and stirring for 20min to obtain gold nanofluorescence probe. The fluorescence emission peak of the gold nanometer fluorescent probe is near 430nm, showing strong blue fluorescence, and the quantum yield is 2.7%.

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PUM

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Abstract

The invention provides a gold-nanoparticle fluorescence probe and a preparation method and application thereof. The preparation method comprises the steps that 1-10 parts of 3-5 mmol / L chloroauric acid aqueous solution is heated to 80 DEG C to 120 DEG C, 1-5 parts of 4-6 mmol / L 2-mercapto-4-methyl-5-thiazoleacetic acid aqueous solution is added to the chloroauric acid aqueous solution, continuous heating stirring is conducted for 5 min to 30 min, and the gold-nanoparticle fluorescence probe is obtained. The preparation method is simple in reaction condition, rapid and friendly to environment. 2-mercapto-4-methyl-5-thiazoleacetic acid serves as a reducing agent and a protective agent, 2-mercapto-4-methyl-5-thiazoleacetic acid itself has the reducing property, and addition of a frequently-used reducing agent sodium borohydride, surfactants and the like is avoided. The gold-nanoparticle fluorescence probe presents intense blue fluorescence on the fluorescence emission peak near 430 nm, the gold-nanoparticle fluorescence probe is good in stability when preserved at room temperature and can be used for high-sensitivity and high-selectivity detection of Fe<3+>, the detection process is simple, convenient and rapid, and the detection result is accurate.

Description

technical field [0001] The invention relates to a fluorescent probe, in particular to a water-soluble blue gold nano fluorescent probe and its preparation method and application. Background technique [0002] Iron is one of the most important components of hemoglobin. It participates in the transport of oxygen and is also essential to maintain the normal functions of other oxygen-carrying proteins such as ferrohemoglobin, myoglobin, and cytochrome. It is also a part of many enzymes involved in energy metabolism It is an important component of human body, which has a serious impact on human health, especially the blood system. Iron deficiency can lead to too few red blood cells and even anemia, and conversely, iron excess can also cause various diseases such as Parkinson's, Alzheimer's and cancer. At present, there are many traditional scientific methods for iron detection, such as atomic absorption spectrometry, inductively coupled plasma mass spectrometry, and gas chromato...

Claims

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Application Information

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IPC IPC(8): C09K11/07G01N21/64
CPCC09K11/07G01N21/6428
Inventor 张彦高鹏飞姜晶晶张国梅双少敏
Owner SHANXI UNIV
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