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Preparation method and application of diterpene alkaloids in Junggar Aconitum

A technology of diterpene alkaloid, Junggar aconitum, applied in the field of phytochemistry

Active Publication Date: 2018-03-20
XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the extraction, separation and structural identification of diterpenoid alkaloids from Junggar aconitum and related pharmacological research are still in the initial stage at home and abroad.

Method used

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  • Preparation method and application of diterpene alkaloids in Junggar Aconitum
  • Preparation method and application of diterpene alkaloids in Junggar Aconitum
  • Preparation method and application of diterpene alkaloids in Junggar Aconitum

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Take 10 kg of the above-ground part of Aconitum aconitum, crush it, soak it with 80% ethanol at room temperature for extraction, concentrate under reduced pressure and recover the ethanol to obtain the total extract;

[0036] The total extract is dispersed in an aqueous sulfuric acid solution with a mass fraction of 2%, filtered, and the obtained aqueous acid solution is treated with anhydrous sodium carbonate, extracted with chloroform, and concentrated in chloroform under reduced pressure to obtain the total alkaloids;

[0037] The total alkaloids were separated by normal-phase silica gel column chromatography, and chloroform, volume ratios of 40:1, 30:1, 20:1, 10:1, 5:1, and 1:1 chloroform-methanol were used as solvents in turn The system carried out gradient elution, and the fractions were analyzed by silica gel thin layer chromatography (TLC). The same fractions were combined to obtain 7 sections of components (A-G). :1:0.1%, 5:1:0.1% and 1:1:0.1% n-hexane-acetone-...

Embodiment 2

[0039] Take 10kg of the aerial part of Aconitum aconitum Junggar, crush it, soak it with 40% ethanol at room temperature for extraction, concentrate under reduced pressure and recover the ethanol to obtain the total extract;

[0040] Disperse the total extract in an aqueous sulfuric acid solution with a mass fraction of 5%, filter, and then treat the obtained aqueous acid solution with anhydrous sodium carbonate, extract with chloroform, and concentrate the chloroform under reduced pressure to obtain the total alkaloids;

[0041]The total alkaloids were separated by normal phase silica gel column chromatography, followed by gradients of chloroform, volume ratio 40:1, 30:1, 20:1, 10:1, 5:1 and 1:1 chloroform-methanol Elution, the fractions were analyzed by silica gel thin layer chromatography, and the same fractions were combined to obtain 7 sections of components (A-G), and component C was separated by normal phase silica gel column chromatography at a volume ratio of 10:1:0.1%...

Embodiment 3

[0043] Take 10kg of the aerial part of Aconitum aconitum Junggar, pulverize it, extract it by percolation with methanol, concentrate under reduced pressure and recover methanol to obtain the total extract;

[0044] The total extract is dispersed in an aqueous hydrochloric acid solution with a mass fraction of 1%, filtered, the obtained aqueous acid solution is treated with anhydrous sodium carbonate, extracted with chloroform, and the chloroform is concentrated under reduced pressure to obtain the total alkaloids;

[0045] The total alkaloids were separated by normal phase silica gel column chromatography, followed by gradients of chloroform, volume ratio 40:1, 30:1, 20:1, 10:1, 5:1 and 1:1 chloroform-methanol Elution, fractions are analyzed by silica gel thin layer chromatography (TLC), and the same fractions are combined to obtain 7 sections of components (A-G), and component C is separated by normal phase silica gel column chromatography, with a volume ratio of 10:1: 0.1%, ...

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Abstract

The invention relates to a preparation method and application of diterpene alkaloid compounds in soongaricum var .pubescens .The method includes the steps that soongaricum var .pubescens medicinal materials serve as raw materials and extracted with a solvent, after acid dissolution and alkali precipitation, solvent extraction is conducted, separation is conducted in two or three modes of silica gel column chromatography, silica gel preparation thin layer chromatography and sephadex LH-20 column chromatography, and three diterpene alkaloid compounds are obtained .The obtained compounds are subjected to in-vitro cell cytotoxic activity determination .The experimental result shows that the iterpene alkaloid compounds 14-benzoyl-8-methoxy chasmanine, neojiangyou aconitine and chasmanine separated from the soongaricum var .pubescens have different degrees of cell cytotoxic activity on human breast cancer cells MCF-7, human lung adenocarcinoma cells A549 and human prostate cancer cells PC-3, and the diterpene alkaloid compounds can be used for preparing anti-tumor drugs .

Description

technical field [0001] The invention relates to the field of phytochemistry, in particular to extracting and separating three diterpene alkaloid compounds with pharmacological activity from the medicinal material of Junggar aconitum. Background technique [0002] Aconitum soonaricum var.pubescens is a kind of plant of the genus Aconitum in the family Ranunculaceae, and it is mainly distributed in Xinjiang (Balkol) and Central Asia in my country. It grows in mountainous forests at an altitude of 2200 meters. The medicinal material is a variant of Aconitum aconitum, which differs from Aconitum aconitum in that both inflorescence rachis and pedicels have developed pubescent hairs. Studies have shown that Aconitum aconitum has important medicinal value. As a traditional folk medicinal plant with a long history of medication, it has analgesic, sedative, rheumatism, treatment of bruises, cough and asthma, antipyretic, etc. effect. [0003] At present, the extraction, separation...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D221/22A61P35/00
CPCC07D221/22
Inventor 阿吉艾克拜尔·艾萨外塔尼古丽·卡米力赵波
Owner XINJIANG TECHN INST OF PHYSICS & CHEM CHINESE ACAD OF SCI