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Method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen

A conversion treatment, high ammonia nitrogen technology, applied in the field of invention related to the ring, can solve the problems related to the absorption capacity

Inactive Publication Date: 2016-08-17
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Higher crops and marine phytoplankton have a tendency to absorb more nitrogen after returning from nitrogen starvation to nitrogen abundance than when nitrogen is always abundant. However, previous studies only focus on the growth and cell composition of microalgae after nitrogen starvation. The growth and nitrogen uptake capacity of microalgae are rarely involved after starvation returns to nitrogen abundance

Method used

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  • Method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen
  • Method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen
  • Method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen

Examples

Experimental program
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Effect test

Embodiment 1

[0041] High ammonia nitrogen tolerant algae strains - local screening of Chlorella sorokiniana, the algae strains can not only grow autotrophically in photosynthesis, but also grow heterotrophically under dark conditions, and can also grow polytrophically under the above conditions at the same time . Its growth characteristics under autotrophic, heterotrophic and polytrophic conditions are as follows: figure 1 shown.

[0042] Wherein the autotrophic medium formula is: K 2 HPO 4 ·3H 2 O 0.04g / L, MgSO 4 ·7H 2 O 0.075g / L, CaCl 2 2H 2 O 0.036g / L, citric acid 0.006g / L, ferric ammonium citrate 0.006g / L, EDTA 0.001g / L, NaNO 3 1.5g / L, Na 2 CO 3 0.02g / L, A5 trace element solution 1.5ml / L, of which A5 trace element solution composition: H 3 BO 3 2.86g / L, MnCl 2 4H 2 O 1.81g / L, ZnSO 4 ·7H 2 O 0.222g / L, NaMoO 4 2H 2 O 0.39g / L, CuSO 4 ·5H 2 O 0.079g / L, and CoCl 2 ·6H 2 O 0.05g / L. where NH 4 The concentration of Cl is 40mg / L, 80mg / L and 160mg / L.

[0043] The formu...

Embodiment 2

[0049] The high ammonia nitrogen wastewater collected by a local municipal wastewater treatment plant in Nanchang has a total nitrogen concentration of 178mg / L and an ammonia nitrogen concentration of 160mg / L. First, the above method is used to perform high-density culture, harvest, nitrogen-limited culture and harvest microalgae cells in sequence, and then transfer to high ammonia nitrogen wastewater for autotrophic culture. The results show that the method of using concurrently nutrient-rich microorganisms of the present invention to convert and process high ammonia nitrogen The method of waste water, in the high ammonia nitrogen waste water that initial concentration is 160mg / L, ammonia nitrogen removal rate can reach 19.1mg / L / d at the highest (such as image 3 shown in c).

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Abstract

The invention discloses a method for nitrogen abundance transformation treatment of wastewater with high ammonia nitrogen by utilizing mixotrophic microorganisms; with use of microbial cells, such as microalgae, photobacteria and other chemical energy bacteria, obtained from pre-culture or harvested from a culture system, mixotrophic microbial cells are subjected to rapid culture accumulation in an abundant nitrogen culture medium, and followed by mixotrophic microorganisms are harvested and are transferred to a nitrogen limited culture medium, are subjected to nitrogen limited culture for 2-4 days, finally are transferred into the wastewater with high ammonia nitrogen and are subjected to autotrophic growth under illumination conditions; because the mixotrophic microorganisms need to synthesize liposome for saving energy in the nitrogen limited process, and simultaneously degrade chloroplast and other organelles, in the abundant nitrogen transformation process under the illumination conditions, a large number of ammonia nitrogen nutrient sources are needed for synthesizing chlorophylls and other assemblies needed for photosynthesis, and thus the high-concentration ammonia nitrogen in the wastewater can be effectively removed, and the purpose of purifying the wastewater is achieved.

Description

technical field [0001] The present invention relates to the technical field of environmental governance, in particular to a method for converting and treating high-ammonia-nitrogen wastewater by utilizing the nitrogen abundance of concomitant microorganisms, specifically using microbial cells such as microalgae and photobacteria obtained from pre-cultivation or harvested from a culture system And other chemical energy bacteria, etc., carry out high-density culture in nitrogen-enriched medium to accumulate accompa Autotrophic growth in high ammonia nitrogen wastewater and under light conditions to efficiently remove high ammonia nitrogen in wastewater. Background technique [0002] The purification of high-ammonia-nitrogen wastewater is a major problem worldwide. The traditional activated sludge method removes ammonia-nitrogen through nitrification-denitrification. Usually, due to insufficient carbon sources, an additional organic carbon source is required, and the constructi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/32C02F3/34C12N1/20C12N1/12C12R1/01C12R1/89
CPCC02F3/32C02F3/34C12N1/12C12N1/20Y02E50/30
Inventor 周文广阮榕生王菁晗黎俊
Owner NANCHANG UNIV
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