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Method for producing volatile fatty acid (VFA) from mushroom cultured residue through alkaline fermentation

A technology for volatile fatty acids and mushroom residues, which is applied in the field of organic solid waste recycling, can solve problems such as land occupation and secondary environmental pollution, and achieve the effects of low operating cost, restraining consumption and good application prospects.

Inactive Publication Date: 2016-08-17
HUNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The stockpiling of mushroom slag not only takes up a lot of land, but also easily causes serious secondary pollution to the environment

Method used

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  • Method for producing volatile fatty acid (VFA) from mushroom cultured residue through alkaline fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) Take a certain amount of mushroom slag from a certain mushroom factory, its C / N=28.69, dry it in an oven at 105°C for 24 hours, then mechanically crush it, sieve it, the particle size is 1.0-0.5cm, and the sieved mushroom dregs as fermentation substrate;

[0020] (2) get the mushroom dregs that obtain in step (1) and put into fermenter, add distilled water and be configured into the mixed solution that solid content rate is 12%; Add inoculated sludge in mixed solution, mixed solution volume and inoculated sludge volume The ratio is 5:1;

[0021] (3) Pass nitrogen into the fermenter of step (2) for 5 minutes and then seal it, and ferment under anaerobic conditions. Control the mechanical stirring speed at 120r / min, control the temperature at 35±1℃, and after anaerobic fermentation for 5 days, the concentration of VFA produced is the highest, which is 2274.16mg / L.

Embodiment 2

[0023] (1) Take a certain amount of mushroom slag from a mushroom factory, its C / N=28.69, dry it in an oven at 105°C for 24 hours, then mechanically pulverize it, sieve it, the particle size is 0.5-0.3cm, and the sieved mushroom dregs as fermentation substrate;

[0024] (2) get the mushroom dregs that obtain in step (1) and put into fermenter, add distilled water and be configured into the mixed solution that solid content rate is 15%; Add inoculated sludge in mixed solution, mixed solution volume and inoculated sludge volume The ratio is 5:1;

[0025] (3) Pass nitrogen into the fermenter of step (2) for 5 minutes and then seal it, and ferment under anaerobic conditions. Control the speed of mechanical stirring at 120r / min, control the temperature at 35±1℃, and after 5 days of anaerobic fermentation, the concentration of VFA produced is the highest, which is 2657.58mg / L.

Embodiment 3

[0027] (1) Take a certain amount of mushroom slag from a certain mushroom factory, its C / N=28.69, dry it in an oven at 105°C for 24 hours, then mechanically crush it, sieve it, the particle size is 1.0-0,5cm, and the sieved mushroom dregs as fermentation substrate;

[0028] (2) get the mushroom dregs that obtain in step (1) and put into fermenter, add distilled water and be configured into the mixed solution that solid content rate is 15%; Add inoculated sludge in mixed solution, mixed solution volume and inoculated sludge volume The ratio is 5:1;

[0029] (3) Pass nitrogen into the fermenter of step (2) for 5 minutes and then seal it, and ferment under anaerobic conditions. Control the mechanical stirring speed at 120r / min, control the temperature at 35±1°C, add 1.0mol / L NaOH solution during the entire anaerobic fermentation process, and maintain the pH of the fermentation system at about 10. After 5 days of anaerobic fermentation, the produced The concentration of VFA is t...

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Abstract

The invention relates to the technical field of resource converting of organic solid wastes and particularly relates to a method for producing volatile fatty acid (VFA) from mushroom cultured residue through alkaline fermentation. The preparation method comprises the following steps: taking the mushroom cultured residue as a fermentation substrate, and taking anaerobically digested sludge as inoculating sludge; carrying out mechanical pulverization on dried mushroom cultured residue, adding distilled water into the powder so as to prepare a mixed solution with the solid content of 12% to 18%, mixing the mixed liquid with the inoculating sludge according to the volume ratio of 5: 1, and introducing nitrogen gas into the mixture for 3 to 5 minutes; controlling the reaction temperature to 35 DEG C and the stirring rate to 120r / min to 180r / min, maintaining the pH of a system to about 10, and carrying out alkaline fermentation on the mushroom cultured residue in an anaerobic environment for 4 to 7 days; carrying out centrifugation on the mixed solution obtained after the alkaline fermentation by adopting a centrifuge at the centrifugal speed of 12,000r / min, so as to obtain centrifugal supernatant, i.e. VFA fermentation liquor. According to the method, the technological operation is simple, anaerobic fermentation conditions are easily controlled, and the economic cost is low, so that the method has a good application prospect. The invention provides a novel method for solving the problem of stacking of the mushroom cultured residue and achieving the resource converting of agricultural wastes and has important significance.

Description

technical field [0001] The invention relates to the technical field of organic solid waste recycling, in particular to a method for producing volatile fatty acids (VFA) by alkaline fermentation using mushroom dregs as raw materials. Background technique [0002] With the development of economy and the increase of population, my country's demand for fossil energy is increasing day by day. At present, China is the country with the largest energy consumption in the world, accounting for 21% of global energy consumption. At the same time, the emissions after the use of fossil energy will bring serious environmental problems such as acid rain and urban smog. In order to solve the problems of energy crisis and environmental degradation, the use of biological anaerobic digestion technology to generate renewable energy has received more and more attention. The anaerobic digestion process is divided into three stages: hydrolysis, acidification, and methanogenesis. Among them, the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/52C12P7/54
CPCC12P7/52C12P7/54
Inventor 张盼月房玮曾光明张海波吴彦刘建波苟玺莹张亚迪许东
Owner HUNAN UNIV
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