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Immunochromatography quantitative detection test paper strip for procalcitonin of terminal blood

A technology of procalcitonin and immunochromatography, which is applied in the field of immunoassay, can solve problems such as difficulty in meeting the minimum sample size of test strips, inability to accurately detect PCT content in peripheral blood, and small amount of peripheral blood sampling, so as to achieve reliable diagnosis Results, beneficial to popularization, and the effect of improving the stability of detection

Inactive Publication Date: 2016-08-17
GUANGZHOU TEBSUN BIO TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the amount of blood collected from peripheral blood is generally relatively small, and it is difficult to meet the minimum sample volume required for test strip detection
Therefore, although there are currently some PCT test strips that can measure the PCT content in venous blood serum or plasma, these test strips cannot accurately detect the PCT content in peripheral blood.

Method used

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  • Immunochromatography quantitative detection test paper strip for procalcitonin of terminal blood
  • Immunochromatography quantitative detection test paper strip for procalcitonin of terminal blood
  • Immunochromatography quantitative detection test paper strip for procalcitonin of terminal blood

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Embodiment 1, calcitonin immunochromatographic quantitative detection test strip 1

[0021] Such as figure 1 As shown in the procalcitonin immunochromatographic quantitative detection test strip, the bottom plate 4 is provided with a sample pad 1 , a nitrocellulose membrane 2 and an absorbent pad 3 in sequence. Biotin-labeled PCT monoclonal antibody (concentration: 0.3~1.5mg / mL) and streptavidin-labeled (or avidin) fluorescent protein (using excitation wavelength 530nm and emission wavelength 570nm) were immobilized on sample pad 1 , concentration 0.1~1.0mg / mL); the test line T (concentration 0.5~3mg / mL) composed of a monoclonal antibody recognizing another epitope of PCT immobilized on the nitrocellulose membrane 2 and goat anti-mouse IgG polyclonal antibody Quality control line C (concentration 0.2~2.0mg / mL), quality control line C is used to test the validity of the test strip. The fluorescent protein is one of green fluorescent protein, phycobiliprotein, and the l...

Embodiment 2

[0023] Embodiment two, calcitonin immunochromatography quantitative detection test strip 2

[0024] Compared with the first embodiment, the second embodiment has the following characteristics: the sample pad 1 is composed of a glass cellulose film and a polyester film; anti-human red blood cell antibodies are added to the sample pad 1 .

Embodiment 3

[0025] Embodiment 3. Improvement of Calcitonin Immunochromatography Quantitative Detection Test Strip I

[0026] Such as image 3 As shown, the following improvements are made on the basis of the test strip in Example 2: the sample pad 1 includes a laminated first sample pad 10 and a second sample pad 11, and one end of the first sample pad 10 is placed on the sample pad 1 Below, the second sample pad 11 is overlapped and pressed on one end of the nitrocellulose membrane 2 .

[0027] Further, a positioning bar 12 is set at the end of the first sample pad 10 inserted below the sample pad, and a positioning groove capable of accommodating the positioning bar 22 is provided on the lower end surface of the sample pad 1 .

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Abstract

The invention discloses an immunochromatography quantitative detection test paper strip for procalcitonin of terminal blood. A base plate of the test paper strip is sequentially provided with a sample pad, a nitrocellulose film and a water absorbing pad; the sample pad is composed of a glass cellulose film and a polyester film, and anti-human erythrocyte antibodies, biotin-labeled PCT monoclonal antibodies and streptavidin-labeled fluorescin are immobilized on the sample pad; a detection line composed of monoclonal antibodies for recognizing another epitope of PCT and a quality control line composed of polyclonal antibodies are immobilized on the nitrocellulose film. The test paper strip is simple in structure and can detect PCT of the terminal blood.

Description

technical field [0001] The invention belongs to the field of immune detection, and in particular relates to a high-sensitivity procalcitonin immunochromatographic quantitative detection test strip that can be used for peripheral blood detection. Background technique [0002] Procalcitonin (PCT) is the propeptide of calcitonin, which is a glycoprotein composed of 116 amino acids with a molecular weight of 13KD and no hormone activity. PCT is expressed by neuroendocrine cells (including thyroid, lung and pancreatic tissue cells), and is decomposed into calcitonin (CT), carboxy-terminal peptide and amino-terminal peptide by enzymatic digestion. PCT has a half-life of 20-24 hours in the human body, good stability, and a very low level in normal human serum. However, the content in the serum of patients with systemic bacterial infection or mold or parasite infection increases rapidly, especially in septic shock, the concentration of PCT increases exponentially, and it can be det...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/558
CPCG01N33/74G01N33/558G01N2333/585
Inventor 吴明王小明陈彦蓉夏坤
Owner GUANGZHOU TEBSUN BIO TECH DEV
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