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Immunologic adjuvant composition and preparation method thereof

A technology of immune adjuvant and composition, which is applied in the direction of biochemical equipment and methods, microorganisms, antiviral agents, etc., and can solve the problems of unsatisfactory immune effect and inability to protect animals

Inactive Publication Date: 2016-08-24
李锁霞
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to various reasons, the immune effect of many vaccines is not very satisfactory at present, and cannot provide complete protection for animals.

Method used

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  • Immunologic adjuvant composition and preparation method thereof
  • Immunologic adjuvant composition and preparation method thereof
  • Immunologic adjuvant composition and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1, the preparation of the linear alkyl ester of acrylic acid

[0047] Dissolve 1 g of Carbomer 907 in 50 ml of the corresponding n-octanol, and heat the solution to 135°C. 50 microliters of sulfuric acid was added and the reaction mixture was maintained at 135°C. The reaction was stopped by rapidly cooling the reaction mixture and adding 1 volume of distilled water. Then, the pH of the solution was adjusted to pH=6, and the pressure was reduced at 80° C. (10 -6 ba) Evaporation of the solvent. The product thus obtained was dissolved in distilled water, dialyzed against distilled water, and then lyophilized to give a product named alkylcarbomer 907(8).

Embodiment 2

[0048] Embodiment 2, the preparation of mycobacterium phlei culture

[0049] Mycobacterium phlei strains were cultured in a fermenter at 37°C with aeration, the pH value was 7.0, and the culture time was 4 days. The medium formula: 1g of yeast extract powder, 3ml of concentrated wort juice, 25g of glucose, Diammonium hydrogen phosphate 3g, dipotassium hydrogen phosphate 0.8g, add water for injection to 1000ml. After inactivating the fermentation broth with formaldehyde solution (0.1%), centrifuge and concentrate, discard the supernatant, take the lower layer of bacteria and resuspend 3 times with an appropriate amount of normal saline, centrifuge to remove impurities, measure water, and calculate the weight of dry bacteria. After homogenizing the physiological saline, perform high-pressure homogenization, and then freeze-dry to obtain the culture of Mycobacterium phlei.

example 3

[0050] Example 3: Vaccine Composition Preparation

[0051] 3.1 Preparation of antigens: Prepare avian influenza antigens and Newcastle disease antigens according to Chinese patent CN101607084, and the virus content before inactivation is 10 8.20 (EID 50 / 0.1ml), the antigen prepared by this method does not constitute a limitation of the present invention, those skilled in the art should understand that adopting the avian influenza and Newcastle disease antigen prepared by other strains or adopting from the commercialized product can still realize the object of the present invention.

[0052] 3.2 Preparation of the vaccine composition: add the alkylcarbomer 907 (8) prepared in Example 1, the Mycobacterium phlei culture prepared in Example 2 and levamisole according to the addition amount in Table 1, and add 3.1 to prepare the inactivated For bird flu and / or Newcastle disease antigen, adjust the volume to 100ml with PBS solution with pH=7.2. Stir at room temperature for 30 min...

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Abstract

The invention provides a vaccine adjuvant composition, wherein the adjuvant consists of acrylic polymers and a water solution of a mycobacterium phlei culture; and the invention provides applications of the adjuvant as a vaccine adjuvant and an immuno-potentiator.

Description

technical field [0001] The invention belongs to the technical field of biological products and relates to an immune adjuvant composition. Background technique [0002] Antigens are defined as foreign substances that, when administered, eg, parenterally, elicit an immune response, including eliciting antibody production. Antibodies are substances contained in blood and other body fluids and tissues that bind to antigens and render them harmless. Antibodies form one of the body's natural defense mechanisms. They are highly specific and kill, bind, or render harmless the antigen that triggers antibody formation. It is known that an immune response can be deliberately stimulated by administering specific antigens through vaccines. This method causes the body to develop an immune memory state, which ensures that the body reacts more rapidly and effectively when it comes into contact with an antigen. [0003] The immunogenicity of an antigen can be increased by administering t...

Claims

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Application Information

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IPC IPC(8): A61K39/39A61K39/145A61K39/17A61K39/215A61K39/12A61P31/14A61P31/16A61K31/429
CPCA61K31/429A61K39/12A61K39/39A61K2039/55511A61K2039/55544C12N2760/16034C12N2760/18134A61K2300/00
Inventor 不公告发明人
Owner 李锁霞
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