Combined mutation breeding method for rape

Abstract

The invention discloses a combined mutation breeding method for rape. The method comprises the steps of: selecting rape seeds in season, conducting soaking with ethanol and hot water respectively, then performing soaking in an aqueous solution added with a-protease, carrying out germination accelerating, subjecting the obtained rape plumules to continuous illumination culture, then transferring the rape plumules into a medium to conduct magnetic induction treatment, taking the plant height and oil output as the indexes to perform preliminary screening to obtain dwarf and high-oil rape seedlings, transferring the screened oil rape seedlings into an SH solid medium, injecting a chemical inducer every 4h-6h to the seedling roots, spraying a nutrient solution to the seedlings every day, carrying out continuous mutagenesis, and transferring the obtained rape plants into nutrient soil, taking the plant height and oil output as the indexes, carrying out repeated orientation and subcultured breeding, and gradually increasing the breeding rate of dwarf and high-oil rape plants. The method provided by the invention employs physical mutagenesis and chemical mutagenesis for combined breeding, and is beneficial to expand the breeding method of rape and improve the breeding efficiency of rape breeding.

Description

technical field

[0001] The invention relates to the field of rapeseed breeding, in particular to a combined mutation breeding method for rapeseed. Background technique

[0002] Rapeseed oil extracted from rapeseed is one of the main edible oils in my country. It is rich in unsaturated fatty acids such as linoleic acid and vitamin E and other nutrients. It can be well absorbed by the body and has a good effect of softening blood vessels and delaying aging. Therefore, the improved breeding of rapeseed has received more and more attention and exploration in order to effectively broaden the genetic background of rapeseed and expand its germplasm resources.

[0003] Mutagenesis breeding can increase the mutation rate of breeding, expand the mutation spectrum, and greatly shorten the breeding period, which has played a great role in the field of breeding in recent years. Mutation breeding includes physical mutation breeding and chemical mutation breeding. Physical mutagenesis is...

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