Combined mutation breeding method for rape

A technology for mutation breeding and rapeseed, applied in the fields of botanical equipment and methods, plant genetic improvement, soilless cultivation, etc. The effect of inducing and increasing the mutation rate

Inactive Publication Date: 2016-08-31
林淑录
View PDF7 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the breeding cycle of this patent is very long, and there are very few natural mutants. It takes a lot of man...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Combined mutation breeding method for rape

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A method for joint mutation breeding of rapeseed

[0034] Include the following steps:

[0035] Step S01, cleaning: select the rapeseed seeds of the current season, remove the seeds with poor properties such as mildew, insect damage, frost, etc., soak the selected seeds in an ethanol solution with a mass fraction of 70% for 12 hours, and remove dust and germs on the surface of the seeds, etc. Then soak in clean water for 30 minutes, remove the seeds, and rinse them twice with clean water.

[0036] Step S02, soaking seeds: Soak the seeds treated in step S01 in hot water at 40°C for 2 hours, remove and wash them, and put them into an aqueous solution containing 1%wt a-protease at a temperature of 30°C for soaking After 3 hours, take them out, wrap the seeds with a damp cloth, put them into an incubator, and germinate them for 48 hours at a temperature of 35° C. to obtain rapeseed sprouts with a sprout length of 3 mm.

[0037] Step S03, low-temperature treatment: transpl...

Embodiment 2

[0042] A method for joint mutation breeding of rapeseed

[0043] Include the following steps:

[0044] Step S01, cleaning: select the rapeseed seeds of the current season, remove the seeds with poor properties such as mildew, insect damage, frost, etc., soak the selected seeds in an ethanol solution with a mass fraction of 75% for 24 hours, and remove dust and germs on the surface of the seeds, Afterwards, soak in clean water for 60 minutes, remove the seeds, and rinse with clean water for 3 times.

[0045] Step S02, soaking seeds: soak the seeds treated in step S01 in hot water at 50°C for 3 hours, remove and wash them, and put them into an aqueous solution containing 3.5%wt a-protease at a temperature of 36°C for soaking After 6 hours, take them out, wrap the seeds with a damp cloth, put them into an incubator, and germinate them for 80 hours at a temperature of 40° C. to obtain rapeseed sprouts with a bud length of 4 mm.

[0046] Step S03, low-temperature treatment: trans...

Embodiment 3

[0051] A method for joint mutation breeding of rapeseed

[0052] Include the following steps:

[0053] Step S01, cleaning: select the rapeseed seeds of the current season, remove the seeds with poor properties such as mildew, insect damage, frost, etc., soak the selected seeds in an ethanol solution with a mass fraction of 73% for 18 hours, and remove dust and germs on the surface of the seeds, Then soak in clean water for 45 minutes, remove the seeds, and rinse with clean water for 3 times.

[0054] Step S02, soaking seeds: Soak the seeds treated in step S01 in hot water at 45°C for 2.5 hours, remove and wash them, and put them into an aqueous solution containing 2.3%wt a-protease at a temperature of 33°C Soak for 4.5 hours, remove, wrap the seeds with a damp cloth, put them in an incubator, and accelerate germination for 64 hours at a temperature of 38° C. to obtain rapeseed sprouts with a sprout length of 3.5 mm.

[0055] Step S03, low-temperature treatment: transplant th...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
Bud lengthaaaaaaaaaa
Bud lengthaaaaaaaaaa
Bud lengthaaaaaaaaaa
Login to view more

Abstract

The invention discloses a combined mutation breeding method for rape. The method comprises the steps of: selecting rape seeds in season, conducting soaking with ethanol and hot water respectively, then performing soaking in an aqueous solution added with a-protease, carrying out germination accelerating, subjecting the obtained rape plumules to continuous illumination culture, then transferring the rape plumules into a medium to conduct magnetic induction treatment, taking the plant height and oil output as the indexes to perform preliminary screening to obtain dwarf and high-oil rape seedlings, transferring the screened oil rape seedlings into an SH solid medium, injecting a chemical inducer every 4h-6h to the seedling roots, spraying a nutrient solution to the seedlings every day, carrying out continuous mutagenesis, and transferring the obtained rape plants into nutrient soil, taking the plant height and oil output as the indexes, carrying out repeated orientation and subcultured breeding, and gradually increasing the breeding rate of dwarf and high-oil rape plants. The method provided by the invention employs physical mutagenesis and chemical mutagenesis for combined breeding, and is beneficial to expand the breeding method of rape and improve the breeding efficiency of rape breeding.

Description

technical field [0001] The invention relates to the field of rapeseed breeding, in particular to a combined mutation breeding method for rapeseed. Background technique [0002] Rapeseed oil extracted from rapeseed is one of the main edible oils in my country. It is rich in unsaturated fatty acids such as linoleic acid and vitamin E and other nutrients. It can be well absorbed by the body and has a good effect of softening blood vessels and delaying aging. Therefore, the improved breeding of rapeseed has received more and more attention and exploration in order to effectively broaden the genetic background of rapeseed and expand its germplasm resources. [0003] Mutagenesis breeding can increase the mutation rate of breeding, expand the mutation spectrum, and greatly shorten the breeding period, which has played a great role in the field of breeding in recent years. Mutation breeding includes physical mutation breeding and chemical mutation breeding. Physical mutagenesis is...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01H1/06A01G31/00
CPCA01H1/06A01G31/00
Inventor 林淑录
Owner 林淑录
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap