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Lung perfusion fluid for perfusing transplant donor lung and preparation method for lung perfusion fluid

A perfusate and perfusion technology, applied in the field of medical biology, can solve the problems of weak lung protection and resuscitation and short perfusion time, achieve good lung protection and resuscitation, meet the needs of perfusion and preservation, and have simple preparation methods

Inactive Publication Date: 2016-09-07
SHANGHAI CHEST HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the confidentiality of foreign high-quality perfusate formulas, the formula and configuration process of domestic lung perfusate fluids cannot meet the needs of lung perfusion for transplantation. The lung protection and resuscitation effects are not strong, and the perfusion time is short.

Method used

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  • Lung perfusion fluid for perfusing transplant donor lung and preparation method for lung perfusion fluid

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Experimental program
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Effect test

Embodiment 1

[0029] Example 1 Preparation of lung perfusate

[0030] Use the following procedure to prepare lung perfusate for transplant donor lung perfusion

[0031] Step 1, preparation of basic solution

[0032] Take 50g of dextran and dissolve it in purified water by heating, add 8g of sodium chloride, 0.4g of potassium chloride, 0.098g of magnesium sulfate, 0.046g of anhydrous disodium hydrogen phosphate, 0.063g of potassium dihydrogen phosphate and 0.9g of glucose, and use Tris- Adjust the pH to 7.5 with HCl buffer, filter with a bacterial filter with a pore size of 0.22um, add purified water and set the volume to 1000ml to obtain the basic solution;

[0033] Step 2, preparation of preperfusate

[0034] Add 0.06g of calcium chloride, 50g of albumin, and 2mg of nitroglycerin to the basic solution obtained in step 1, adjust the pH to 7.4-7.6 with a Tris-HCl buffer, and filter with a bacterial filter with a pore size of 0.22um to obtain the pre- perfusate;

[0035] Step 3. Preparati...

Embodiment 2

[0037] Embodiment 2 Pig lung perfusion experiment of the lung perfusate of the present invention

[0038] Pig donor lungs were obtained, intubated and connected to a ventilator. The pulmonary artery and left atrial appendage are directly cannulated and connected to the perfusion circuit. Put the two perfusion tubes flat first, pour out the liquid inside, and then check whether the pressure measuring tube is full of liquid and can flow into the thick perfusion tube. At this time, open the three-joint tube to be the same as the atmosphere. Perfusion, perfusion uses the lung perfusate prepared in Example 1.

[0039] At the beginning of the cycle, the temperature was 12-13°C. When the temperature of the perfusate reached 32°C, ventilator ventilation was performed with an oxygen concentration of 0.21%, starting with a tidal volume of 7ml / kg, 7 breaths / min and a PEEP frequency of 5cmH2O. Deoxygenation of the perfusate will begin with a gas mixture of nitrogen (86%), carbon dioxide...

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Abstract

The invention provides lung perfusion fluid for perfusing a transplant donor lung and a preparation method for the lung perfusion fluid. The lung perfusion fluid can be used for providing good lung protecting and resuscitating actions and can meet the requirements of the transplant donor lung on perfusion and preservation; meanwhile, the time of perfusion is relatively long, and the donor lung still can retain good lung compliance and oxygenation capability 6 hours after the donor lung is subjected to perfusion by the lung perfusion fluid provided by the invention; and finally, the preparation method for the lung perfusion fluid is simple, and raw materials are readily available and are economical and practical, so that the lung perfusion fluid is suitable for being popularized and applied to general laboratories or hospitals.

Description

technical field [0001] The invention relates to the field of medical biotechnology, in particular to a lung perfusate used for transplantation for lung perfusion and a preparation method thereof. Background technique [0002] Lung transplantation (Lung Transplantation, LTx) has become the standard treatment for end-stage lung diseases that cannot be treated by drugs or surgery. At present, the number of donor lungs that meet the standards can no longer meet the demand for transplantation, resulting in the death of some patients while waiting for the donor lungs. Among the current methods that can improve the shortage of donor lungs, ex vivo lung perfusion (Ex Vivo Lung Perfusion, EVLP) is a technology used in the field of lung transplantation to prolong the preservation time of donor lungs and repair the damage of donor lungs. EVLP can immediately assess donor lung function, provide a platform for drugs and even gene therapy, improve the function of "Marginal Donor Lungs", ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 潘雁吴东进赵珩李欣叶波孙益峰顾畅
Owner SHANGHAI CHEST HOSPITAL
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