Method for preparing enzyme from kudzuvine roots and mulberry fruits
A kind of enzyme and mulberry technology, applied in the fields of application, function of food ingredients, food science, etc., can solve the problems of poor health care effect, single fruit, single effect of enzyme, etc., to improve heart and brain blood circulation, stable quality, and simple process Effect
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Embodiment 1
[0020] A kind of ferment of present embodiment, comprises the raw material of following parts by weight to make: 50 parts of kudzu root, 40 parts of mulberry, 15 parts of edible sugar, 3 parts of compound enzyme, 0.15 part of ferrous carbonate.
[0021] A kind of method utilizing kudzu root, mulberry to prepare ferment of the present embodiment, comprises the steps:
[0022] (1) Break the kudzu root and mulberry with a beater and mix them evenly; pour the mixture into a fermentation container, add pre-cooled disodium hydrogen phosphate-citric acid buffer solution, add 2 parts of cellulase, pectinase 1 part and ferrous carbonate, enzymatic hydrolysis at 15°C for 60 minutes, pH value 5.5, standing for extraction, filtering, and taking the filtrate;
[0023] (2) Add purified water of 2 times the weight of the filtrate in the fermentation vessel, add brown sugar while stirring, seal it, and ferment for 5 days under temperature control at 35° C. under dark conditions to obtain the ...
Embodiment 2
[0028] A kind of ferment of present embodiment, comprises the raw material of following parts by weight and is made: 70 parts of kudzu root, 60 parts of mulberry, 32 parts of edible sugar, 6 parts of compound enzyme, 0.6 part of ferrous carbonate.
[0029] A kind of method utilizing kudzu root, mulberry to prepare ferment of the present embodiment, comprises the steps:
[0030] (1) Pueraria root and mulberry are crushed by a beater and mixed evenly; the mixture is poured into a fermentation container, and pre-cooled disodium hydrogen phosphate-citric acid buffer solution is added, 5 parts of cellulase, pectinase 1 portion and ferrous carbonate, enzymatic hydrolysis at 20°C for 40 minutes, pH value 6.5, standing for extraction, filtering, and taking the filtrate;
[0031] (2) Add purified water 4 times the weight of the filtrate into the fermentation container, add white sugar while stirring, seal it, and ferment at 40° C. under temperature control for 2 days in the dark to obt...
Embodiment 3
[0036] A kind of ferment of present embodiment, comprises the raw material of following parts by weight and is made: 60 parts of kudzu root, 50 parts of mulberry, 25 parts of edible sugar, 5 parts of compound enzymes, 0.4 part of ferrous carbonate.
[0037] A kind of method utilizing kudzu root, mulberry to prepare ferment of the present embodiment, comprises the steps:
[0038] (1) Pueraria root and mulberry are crushed by a beater and mixed evenly; the mixture is poured into a fermentation container, and pre-cooled disodium hydrogen phosphate-citric acid buffer solution is added, 4 parts of cellulase, pectinase 1 portion and ferrous carbonate, enzymatic hydrolysis at 18°C for 50 minutes, pH value 6.0, standing for extraction, filtering, and taking the filtrate;
[0039] (2) Add purified water with 3 times the weight of the filtrate in the fermentation vessel, add white sugar and brown sugar while stirring, seal it, and ferment under temperature control at 37°C for 4 days u...
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