Prinsepia utilis royle puree cosmetic and preparation method and application thereof

A technology of fermenting puree and green thorn fruit, which is applied in the biological field, can solve the problems of low safety, long time consumption, and low extraction rate, and achieve the effects of simplifying production steps, simple operation, and enhancing cell vitality

Active Publication Date: 2016-09-07
BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Traditional extraction techniques include organic solvent extraction, hot water extraction,...

Method used

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  • Prinsepia utilis royle puree cosmetic and preparation method and application thereof
  • Prinsepia utilis royle puree cosmetic and preparation method and application thereof

Examples

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preparation example Construction

[0044] The preparation method of the green thorn fruit dry powder: take the green thorn fruit (fruit), fully dry and pulverize, then pass through a 50-mesh sieve to obtain the green thorn fruit dry powder. The green thorn fruit was purchased from Yunnan Baiyao Company, and the place of production is Yunnan.

[0045] Crude polysaccharide detection method: detect and calculate according to the acid hydrolysis method in GB / T 5009.8-2008.

[0046] Detection method of total flavonoids: detection and calculation according to GB / T 20574-2006 (spectrophotometric colorimetry).

Embodiment 1

[0047] Embodiment 1, prepare green thorn fruit fermented protoplasm

[0048] 1. Preparation of yeast liquid

[0049] Inoculate a single colony of Saccharomyces cerevisiae into 100ml of YPD medium, culture at 25°C and shake at 150rpm to OD 600nmValue=0.5-1.0, obtain saccharomyces bacterium liquid (concentration of saccharomyces cerevisiae in saccharomyces bacterium liquid is 10 5 ~10 8 CFU / mL).

[0050] 2. Preparation of green sorrel fermented puree

[0051] 1. Mix 5-15mL of the yeast liquid obtained in step 1, 15-25g of thorn fruit dry powder and 280-350mL of water, then shake and culture at 25-35°C for 46-53 hours, and then collect the entire fermentation system, which is fermentation product.

[0052] 2. Take the fermentation product obtained in step 1 and sterilize at 115-121°C for 15-25min.

[0053] 3. Take the product of step 2, centrifuge at 3800-4200rpm for 10-15min, and collect the supernatant, which is the fermented puree of the green sorrel fruit.

[0054] 3. ...

Embodiment 2

[0058] Embodiment 2, prepare green thorn fruit fermented protoplasm

[0059] 1. Preparation of yeast liquid

[0060] Inoculate a single colony of Saccharomyces cerevisiae into 100ml of YPD medium, culture at 25°C and shake at 150rpm to OD 600nm Value=0.75, obtain saccharomyces bacterium liquid (concentration of Saccharomyces cerevisiae in saccharomyces bacterium liquid is 10 7 CFU / mL).

[0061] 2. Preparation of green sorrel fermented puree

[0062] 1. Mix 8mL of the yeast liquid obtained in step 1, 20g of thorn fruit dry powder and 300mL of water, then shake and culture at 28°C and 180rpm for 48 hours, and then collect the entire fermentation system, which is the fermentation product.

[0063] 2. Take the fermentation product obtained in step 1, and sterilize it at 121°C for 20 minutes.

[0064] 3. Get the product of step 2, centrifuge at 4000rpm for 10min (centrifugal radius is 9cm), collect the supernatant, which is the fermented protoplasm of green sorrel fruit.

[00...

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Abstract

The invention discloses prinsepia utilis royle puree cosmetic and a preparation method and application thereof. The method for preparing prinsepia utilis royle puree includes steps of fermenting prinsepia utilis royle as base by brewer's yeast as strain. The prinsepia utilis royle puree prepared by this method also falls into the scope of this invention. The invention further discloses a product, and its active ingredient is the prinsepia utilis royle puree. The product has functions of (a) and/or (b) and/or (c), wherein (a) for oxidation resistance, (b) for capability of removing DPPH free radicals, and (c) for ageing resistance. By the fermenting technology, active substances can be extracted from prinsepia utilis royle, operation is simple and without any additives, the produced prinsepia utilis royle yeast, namely the prinsepia utilis royle puree, has no harm to skin. The product contains polysaccharide or flavone-rich matters, and fermenting ingredients in fermenting filtrate have synergetic action, and have excellent anti-oxidation and anti-ageing function.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a green sorrel fermented puree cosmetic and its preparation method and application. Background technique [0002] Green thorn fruit, also known as green thorn point, is an evergreen shrub of the genus Rosaceae. Produced in Yunnan, Guizhou, Sichuan, and Tibet. It grows on hillsides, wastelands, valleys or roadsides at an altitude of 1000-2560 meters. It is also distributed in Pakistan, Nepal, Bhutan and northern India. The green thorn tip was first recorded in "Southern Yunnan Materia Medica": "Slightly cold in nature, bitter in taste. Attack all sores and carbuncles"; now there are also literature records: This product "Roots, stems, leaves, fruits: bitter in taste, slightly cold in nature , non-toxic. The stems and leaves are mainly used to treat carbuncle and ulcer, toothache caused by wind and fire, gunshot wounds, fractures, and snake bites; the roots are mainly us...

Claims

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Application Information

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IPC IPC(8): A61K8/97A61Q19/08
CPCA61K8/97A61K2800/85A61Q19/08
Inventor 李萌王昌涛赵丹张佳婵王冬冬
Owner BEIJING TECHNOLOGY AND BUSINESS UNIVERSITY
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