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Soil heavy metal treating agent and application thereof

A heavy metal and treatment agent technology, applied in the field of soil treatment, can solve pollution and other problems, achieve the effect of improving soil structure, reducing the content of heavy metals, and reducing heavy metals

Active Publication Date: 2016-09-07
SHANDONG AGRI UNIV FERTILIZER TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the problem of soil heavy metal pollution, the invention provides a soil heavy metal treatment agent, which can quickly reduce the content of heavy metals in the soil and improve the soil structure

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 A soil heavy metal treatment agent,

[0023] It is composed of Bacillus subtilis fermentation broth, montmorillonite, furfural residue, gypsum powder and humic acid.

[0024] The method for preparing the upper treatment agent, the specific steps are as follows:

[0025] (1) Preparation of Bacillus subtilis slope

[0026] Inoculate the strains on a slant medium for slant culture, and cultivate for 7 days at 32°C and 70% saturation;

[0027] (2) Bacillus subtilis seed culture

[0028] Make a bacterial suspension from the strains cultured on the slope, and inoculate it into the seed culture medium in an amount of 1.5 (volume ratio), turn on the stirring speed of 200 rpm, and cultivate at 32°C until the optical density of the medium is 0.5;

[0029] Seed medium: LB medium: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH 7.2.

[0030] (3) Fermentation culture of Bacillus subtilis

[0031] Inoculate the seed liquid into the fermentation medium at a volume ratio of 1.5), and cul...

Embodiment 2

[0038] Example 2 A soil heavy metal treatment agent

[0039] It is composed of Bacillus subtilis fermentation broth, montmorillonite, furfural residue, gypsum powder and humic acid.

[0040] The method for preparing the upper treatment agent, the specific steps are as follows:

[0041] (1) Preparation of Bacillus subtilis slope

[0042] Inoculate the strains on a slant medium for slant culture, and cultivate for 6 days at 32°C and 70% saturation;

[0043] (2) Bacillus subtilis seed culture

[0044] Make a bacterial suspension from the strains cultured on the slope, and inoculate it into the seed culture medium in an amount of 2% (volume ratio), turn on the stirring speed of 200 rpm, and cultivate it at 32°C until the optical density of the culture medium is 0.5;

[0045] Seed medium: LB medium: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH 7.2.

[0046] (3) Fermentation culture of Bacillus subtilis

[0047] Inoculate the seed liquid into the fermentation medium in an amount of 2% (volum...

Embodiment 3

[0054] Example 3 A soil heavy metal treatment agent

[0055] It is composed of Bacillus subtilis fermentation broth, montmorillonite, furfural residue, gypsum powder and humic acid.

[0056] The method for preparing the upper treatment agent, the specific steps are as follows:

[0057] (1) Preparation of Bacillus subtilis slope

[0058] Inoculate the strains on the slant medium for slant culture, and cultivate for 7 days at 32°C and 70% saturation;

[0059] (2) Bacillus subtilis seed culture

[0060] Make a bacterial suspension from the strains cultured on the slope, and inoculate it into the seed culture medium according to the amount of 1.5% (volume ratio), turn on the stirring speed of 200 rpm, and cultivate at 32°C until the optical density of the culture medium is 0.5;

[0061] Seed medium: LB medium: peptone 10g / L, yeast extract 5g / L, NaCl 10g / L, pH 7.2.

[0062] (3) Fermentation culture of Bacillus subtilis

[0063] The seed liquid was inoculated into the fermentation medium at a rat...

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PUM

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Abstract

The invention relates to a soil heavy metal treating agent and an application thereof. The soil heavy metal treating agent consists of bacillus subtilis fermentation liquor, montmorillonite, furfural residues, gypsum powder and humic acid. According to the soil heavy metal treating agent, by virtue of special fermentation culture on bacillus subtilis, cell walls of the bacillus subtilis contain rich teichoic acid and peptidoglycan. Moreover, the montmorillonite, the furfural residues, the gypsum powder and the humic acid are combined, so that heavy metals in the soil can be quickly and efficiently reduced by the soil heavy metal treating agent.

Description

Technical field [0001] The invention relates to a soil heavy metal treatment agent and its application, and belongs to the technical field of soil treatment. Background technique [0002] The soil is the source and sink of heavy metal ions. The metal ions in the soil can exist in various forms, such as soluble state, exchange state, and combined with different soil solid components such as carbonate, iron and manganese oxide, organic matter, and residue materials. Shape. Soil microorganisms are active colloids in the soil. They have a large surface area, are charged with a strong metabolic activity, and are contaminated by heavy metals. They are often enriched with a variety of heavy metal-resistant fungi and bacteria. Microbes can be affected by a variety of modes of action. The following aspects: (1) biosorption and enrichment, (2) dissolution and precipitation, (3) oxidation-reduction, (4) the bioavailability relationship between mycorrhizal fungi and soil heavy metals. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B09C1/10C12N1/20C12R1/125
CPCB09C1/10C12N1/20
Inventor 丁方军路艳艳洪丕征陈士更吴钦泉朱福军郭新送胡翠红陈大印
Owner SHANDONG AGRI UNIV FERTILIZER TECH CO LTD
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