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Method of construction for photoelectrochemical glucose oxidase sensor with graphite like g-C3N4-TiO2 nanosheet composite as enzymatic molecule immobilization scaffold

A technology of g-c3n4-tio2 and glucose oxidase, which is applied in the field of photoelectrochemical enzyme sensor construction, can solve the problems of photogenerated charge hindering the application, and achieve the effects of promoting electron transfer, strong biocompatibility, and high sensitivity

Inactive Publication Date: 2016-09-07
HENAN UNIVERSITY
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Problems solved by technology

However, the rapid recombination of photogenerated charges severely hinders the g-C 3 N 4 Applications in Photoelectrochemical Sensors

Method used

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  • Method of construction for photoelectrochemical glucose oxidase sensor with graphite like g-C3N4-TiO2 nanosheet composite as enzymatic molecule immobilization scaffold
  • Method of construction for photoelectrochemical glucose oxidase sensor with graphite like g-C3N4-TiO2 nanosheet composite as enzymatic molecule immobilization scaffold
  • Method of construction for photoelectrochemical glucose oxidase sensor with graphite like g-C3N4-TiO2 nanosheet composite as enzymatic molecule immobilization scaffold

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Embodiment 1

[0032] A graphite-like phase g-C 3 N 4 -TiO 2 The nano sheet composite material is the construction method of the photoelectrochemical glucose oxidase sensor of enzyme molecule immobilized support, and it comprises the following steps:

[0033] ①Graphite-like phase g-C 3 N 4 Preparation of:

[0034] Weigh 5.0 g of melamine powder into a crucible, then place the crucible in a muffle furnace, heat up to 550 °C at a heating rate of 5 °C / min, keep at 550 °C for 4 h, cool naturally to room temperature, and then Grind the yellow solid to powder to get g-C 3 N 4 powder;

[0035] ②g-C 3 N 4 -TiO 2 Preparation of composite materials:

[0036] 40 mg g-C 3 N 4 The powder was dispersed in 40 mL of isopropanol, ultrasonicated for 30 min, then 0.03 mL of diethylenetriamine was added and stirred for 5 min, then 1.8 mL of titanium isopropoxide was added, stirred evenly and transferred to 40 mL of polytetrafluoroethylene In the reactor, react at 200° C. for 24 h; after the reacti...

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Abstract

The invention discloses a method of construction for a photoelectrochemical glucose oxidase sensor with a graphite like g-C3N4-TiO2 nanosheet composite as an enzymatic molecule immobilization scaffold. The method comprises the steps of firstly preparing g-C3N4 by means of a thermal polymerization method, then preparing the g-C3N4-TiO2 composite by means of a hydrothermal method, and finally modifying a surface of an ITO electrode with the synthesized g-C3N4-TiO2 composite and glucose oxidase (GOD) together with Nafion as a binder to construct the GOD sensor. The photoelectrochemical glucose oxidase sensor prepared by means of the method provided by the invention is capable of fast glucose determination, and has the advantages of relatively high sensitivity, relatively wide linear range, relatively low detection limit, and the like.

Description

technical field [0001] The invention belongs to the technical field of photoelectrochemical enzyme sensor construction, in particular to a graphite-like phase g-C 3 N 4 -TiO 2 The nanosheet composite material is a construction method of a photoelectrochemical glucose oxidase sensor that is an enzyme molecule immobilized scaffold, and the photoelectrochemical enzyme sensor can be used to detect glucose. Background technique [0002] In recent years, due to its advantages of high sensitivity, low energy consumption and good reproducibility, photoelectrochemistry has been used as a new analytical technique with great application potential in the fields of biology and biochemical analysis. In the photoelectrochemical detection process, light is used as an excitation source to excite the photosensitive substance to generate electrons and holes on the electrode surface, and at the same time, a voltage is applied to separate the photogenerated charges to generate a photocurrent, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416
CPCG01N27/416
Inventor 刘小强刘培培霍小鹤朱杰
Owner HENAN UNIVERSITY
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