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Uric acid electrochemical sensor based on active copper carbon point and application thereof

An electrochemical and sensor technology, applied in the field of carbon dot electrochemical sensors, can solve the problems of uric acid sensitivity not meeting demand, ascorbic acid interference, etc., and achieve the effect of strong electron transfer rate, high electron transfer rate, and large specific surface area.

Active Publication Date: 2017-08-29
艾科思电子科技(常州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the methods for detecting uric acid with electrochemical sensors in the prior art mainly encounter two problems: on the one hand, the oxidation potential of the traditional electrode is close to that of ascorbic acid, which is easily interfered by ascorbic acid; on the other hand, the sensitivity of uric acid detection is limited and cannot meet the requirements. need

Method used

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  • Uric acid electrochemical sensor based on active copper carbon point and application thereof
  • Uric acid electrochemical sensor based on active copper carbon point and application thereof
  • Uric acid electrochemical sensor based on active copper carbon point and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation method of electrochemical biosensor based on carbon dots containing active copper includes the following steps:

[0032] A. Preparation of carbon dots containing activated copper: use hydrothermal method to synthesize carbon dots containing activated copper in one step: first weigh 2.5g PMAA and 50mg Cu(NO 3 ) 2 In a beaker, add 50ml of deionized water to dilute, and pour it into a hydrothermal reaction kettle after mixing uniformly, and react at 240°C for 9 hours. The resulting product was centrifuged at 13000 rpm for 30 minutes to remove large particles in the solution. Use a dialysis bag with a molecular weight of 1000D to dialyze for 12 hours to achieve the purpose of purification, and finally store at 4°C in the dark. The obtained carbon dots containing activated copper were characterized by fluorescence spectroscopy and ultraviolet-visible absorption spectroscopy, and the results are as follows figure 2 Shown.

[0033] B. Pretreatment of glassy carbon...

Embodiment 2

[0038] Add 50mg Cu(NO 3 ) 2 Becomes 1500mg Cu(NO 3 ) 2 , Other conditions are the same as in Example 1 to prepare an electrochemical biosensor based on carbon dots containing active copper.

Embodiment 3

[0040] Add 50mg Cu(NO 3 ) 2 Becomes 36.4mg CuCl 2 , Other conditions are the same as in Example 1 to prepare an electrochemical biosensor based on carbon dots containing active copper.

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Abstract

The invention discloses a uric acid electrochemical sensor based on an active copper carbon point and an application thereof. The application comprises the following steps: adding a high molecular polymer rich in carboxyl and cupric salt into a reaction container, putting the reaction container in a hydrothermal reaction kettle, performing a reaction for 2-18h at 200-240 DEG C, performing centrifugalization and dialysis and purification to obtain an active copper carbon dot-containing solution; by adopting a three-electrode system, taking a glass carbon electrode as a working electrode, a platinum wire electrode as a counter electrode and a saturated mercurous chloride electrode as a reference electrode, putting the pre-treated glass carbon electrode in the active copper carbon dot-containing solution, and performing scan round at the scanning speed of 50-500mV / s in a potential range of 0-2V by means of an electrodeposition method. Compared with the prior art, the nanomaterial containing the active copper carbon dots not only has a large specific surface area of the carbon dots and a high electron transmission rate, but also a promoting action on the electron transfer rate on a matrix on an electrode interface by active copper and very strong selectivity on uric acid.

Description

Technical field [0001] The invention belongs to the technical field of electrochemical biosensors, and specifically relates to an electrochemical sensor based on carbon dots containing active copper and an application thereof to realize selective and sensitive detection of uric acid. Background technique [0002] Uric acid is a metabolite of human purines. Excessive levels in the human body can cause gouty arthritis, hyperuricemia and Lesh Nyan syndrome. In recent years, with the improvement of people's quality of life, the content of purines ingested by the Chinese people from food has increased, and more and more people are in a sub-healthy state or even a disease state with high uric acid. Therefore, the accurate detection of uric acid concentration in the human body is extremely important in the field of clinical diagnosis. [0003] At present, the methods for determining the concentration of uric acid mainly include chromatography, spectrophotometry, fluorescence, chemilumine...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/48
CPCG01N27/48
Inventor 刘红英马焕焦名儒金腾飞戴大响黄成黄志恒
Owner 艾科思电子科技(常州)有限公司
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