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Walnut oligopeptide, and preparation technology and use thereof

A technology of walnut oligopeptide and content, applied in the biological field, can solve problems such as difficulty and low utilization rate of walnut protein, and achieve the effect of relieving brain fatigue

Active Publication Date: 2016-09-14
SINPHAR TIAN LI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The theoretical yield of walnut oligopeptides is about 35%, so it is difficult to increase the yield of peach oligopeptides by one percentage point on the existing basis
Productivity Using neutral or neutral papaya compound protease to hydrolyze, the yield of walnut oligopeptides is about 20%, while the yield of oligopeptides obtained by using other proteolysis is 10-17%, resulting in low utilization rate of walnut protein

Method used

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  • Walnut oligopeptide, and preparation technology and use thereof
  • Walnut oligopeptide, and preparation technology and use thereof
  • Walnut oligopeptide, and preparation technology and use thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Peptide powder obtained by a single enzymatic hydrolysis method: divide 300kg of cold-pressed and degreased walnut meal into three equal parts, the first equal part of walnut meal (recorded as A) is mixed with water at a weight ratio of 1:10, and the pH is adjusted to 10 Extract at room temperature for 2 hours; after the extraction is completed, filter and filter the residue for secondary extraction, pour the filtrate into the second aliquot of walnut meal (recorded as B), adjust the pH to 10 and extract at room temperature for 2 hours; after B completes the first extraction, The filtrate is ready for use, and the filter residue continues to be extracted for the second time; after A completes the second extraction, the filter residue is discarded, and the filtrate is poured into the third equal portion of walnut meal (recorded as C), and the pH is adjusted to 10 and extracted at room temperature for 2 hours; B is completed After the second extraction, the filter residue ...

Embodiment 2

[0043] Peptide powder obtained by a single enzymatic hydrolysis method: mix 100 kg of cold-pressed degreased walnut meal with water at a weight ratio of 1:10, adjust the pH to 9.5, extract at room temperature twice, each time for 2 hours, and combine the two alkaline extracts, Centrifugal filtration, adjust the pH of the filtrate to 5, let it stand for 6 hours, discard the supernatant, and finally add water with a volume ratio of 1:10 to the precipitate, and heat to 50°C, adjust the pH value to neutral, add 1kg of papain (enzyme activity: 500,000 u / g), stirred for 5 hours, boiled to inactivate for 30 minutes, centrifuged, and the supernatant was the proteolysis solution. The proteolysis solution was filtered with a microfiltration membrane with a pore size of 0.1 μm, and the permeate was treated with a 5000 Dalton ultrafiltration membrane, and the retentate was concentrated at 80°C to a solid content of 4.1%, then spray-dried, and the inlet temperature was 140°C, the outlet te...

Embodiment 3

[0046] Peptide powder obtained by a single enzymatic hydrolysis method: mix 100 kg of cold-pressed degreased walnut meal with water at a weight ratio of 1:10, adjust the pH to 9.5, extract at room temperature twice, each time for 2 hours, and combine the two alkaline extracts, Centrifugal filtration, adjust the pH of the filtrate to 5, let it stand for 6 hours, discard the supernatant, and finally add water with a volume ratio of 1:10 to the precipitate, and heat to 55°C, adjust the pH value to neutral, add 1kg bromelain (enzyme activity is 1 million u / g), stirred for 6 hours, boiled to inactivate for 30 minutes, centrifuged, and the supernatant was the proteolysis solution. The proteolysis solution is filtered with a microfiltration membrane with a pore size of 0.1 μm. After the permeate is treated with a 5000 Dalton ultrafiltration membrane, the retentate is concentrated at 80°C to a solid content of 3.7%, and then spray-dried. The inlet temperature is 140°C, the outlet temp...

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Abstract

The invention relates to a walnut oligopeptide, and a preparation technology and a use thereof. The peptide content of above oligopeptide powder is 70wt% or above, and the molecular weight of 85% or above of the walnut peptide is smaller than 1500Dalton. The technology is mainly characterized in that the walnut oligopeptide powder is obtained through two-shot enzymatic hydrolysis, and the yield is improved by 4-9%. The walnut oligopeptide powder has good anti-oxidation activity, has a good protection effect on neuron cells, can be used to prevent or treat the Parkinson's disease and relevant diseases, and can alleviate brain fatigue.

Description

technical field [0001] The invention relates to a production process of walnut oligopeptides, in particular to a preparation process for improving the yield of walnut oligopeptides, and belongs to the field of biotechnology. Background technique [0002] Walnut has another name called walnut, scientific name Juglans regia L, Juglans genus, and cashew nut, hazelnut, almond and claim world four big dry fruits. In "Taiping Yulan", it is said that "the walnut was originally born in Xiqiang. It is strong on the outside and soft on the inside. Its quality is like an ancient sage. It is intended to be a tribute." In ancient times, Tibetans were called Qiang people, so they are also known as Qiang peaches. The whole body of the walnut plant is precious, except that its nuts are edible, and walnut branches, walnut flowers, walnut skin, walnut shells, walnut leaves, etc. can also be used as medicine. Modern medicine has shown that walnuts have a good curative effect on diseases such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34C07K1/30A23L33/18A61K38/01A61P39/06A61P25/16A61P35/00
CPCA23V2002/00A61K38/011C07K1/30C07K1/34C12P21/06A23V2200/30A23V2200/308
Inventor 王昭日刘明川杨胜杰洪达金志强杨进平刘敏
Owner SINPHAR TIAN LI PHARMA
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