Regulation and control device for eNOS (Endothelial nitric oxide synthase) expression and activation and treatment device for peripheral arterial diseases

A peripheral arterial disease and control device technology, applied in the field of medical devices, can solve problems such as low quality of life, unsatisfactory drug treatment effect, and difficulty in obtaining curative effect for patients, and achieve efficient treatment, low treatment costs, and promotion of upregulation

Inactive Publication Date: 2016-09-21
段俊丽 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, although drug therapy, surgical bypass surgery and catheter interventional therapy have undergone tremendous changes, each treatment has its own strict indications. For patients with serious comorbidities (diabetes, severe renal failure and / or cardiac failure) are often not suitable for surgery or catheter interventional therapy, and the effect of drug therapy is not satisfactory, most patients are difficult to obtain satisfactory curative effect, and the quality of life is very low [HIRSCH A T, HASKAL Z J, HERTZER N R, et al.[J] .Circulation,2006,113(11):e463-654]

Method used

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  • Regulation and control device for eNOS (Endothelial nitric oxide synthase) expression and activation and treatment device for peripheral arterial diseases
  • Regulation and control device for eNOS (Endothelial nitric oxide synthase) expression and activation and treatment device for peripheral arterial diseases
  • Regulation and control device for eNOS (Endothelial nitric oxide synthase) expression and activation and treatment device for peripheral arterial diseases

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Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1, in vitro therapeutic ultrasound (TUS) intervention human umbilical vein endothelial cell experiment

[0030] Experimental cells: The human umbilical vein endothelial cell line (HUVECs) used in this experiment was purchased from ATCC in the United States. The cell morphology under the microscope showed a typical paving stone-like shape with good refraction and clear borders. The 3-5 passage cells were mainly used in this study.

[0031] 1. Intervention of HUVECs with different doses of therapeutic ultrasound (TUS)

[0032] 1) HUVECs were plated one day before the experiment to ensure that the cell density in each culture dish (35 mm in diameter) was similar, and then placed in a saturated humidity 37°C, 5% CO2 incubator for overnight culture, and the fresh medium was replaced before TUS intervention the next day.

[0033] 2) The ultrasonic generator used in this experiment is provided by the Institute of Acoustics of Tongji University. The technical parame...

Embodiment 2

[0047] Embodiment 2 in vivo experiments

[0048] Experimental animals: 24 healthy male adult C57BL / 6 mice, weighing 18-22 g, were purchased from Shanghai Slack Experimental Animal Co., Ltd., and were kept in the SPF animal room of Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine.

[0049] 2. Experimental method

[0050] 2.1 Establishment of mouse animal model of hindlimb ischemia

[0051] 1) C57BL / 6 mice were weighed and injected intraperitoneally with 4% chloral hydrate solution (0.1ml / 10g) for general anesthesia, routine skin preparation and disinfection of the left hind limb, and fixed on the operating board in the supine position.

[0052] 2) Make a longitudinal incision from the left groin to the knee joint, separate the subcutaneous fascia layer by layer, clearly expose the femoral artery, vein and femoral nerve below the groin, carefully separate the femoral nerve (to avoid nerve damage), and then remove it from the femoral From the origin...

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Abstract

The invention relates to the technical field of medical equipment, and particularly relates to a regulation and control device for eNOS (Endothelial nitric oxide synthase) expression and activation. The regulation and control device disclosed by the invention for eNOS (Endothelial nitric oxide synthase) expression and activation comprises an ultrasonic irradiation component which irradiates ultrasonic waves to a subject. The regulation and control device, the treatment device and a treatment method disclosed by the invention are not only low in treatment expenditure, but also ensure the safety. In addition, the stability is high, and up-regulation of eNOS expression and activation in a living body can be effectively promoted, so that peripheral arterial diseases are treated effectively.

Description

technical field [0001] The invention relates to the technical field of medical devices, in particular to a device for regulating eNOS expression and activation and a device for treating peripheral arterial diseases including the device for regulating eNOS expression and activation. Background technique [0002] Peripheral artery disease (PAD) is a type of disease mainly caused by atherosclerosis involving the peripheral arterial vascular bed, resulting in blood flow obstruction and tissue ischemia, with an overall incidence of about 3%-10%. The lower extremities are the predilection site for PAD, and 1%-2% of PAD patients over 50 years old will develop into critical limb ischemia (CLI), with rest pain, ischemic ulcer and / or gangrene and other clinical symptoms. CLI has become one of the diseases that seriously endanger human health[TONGERS J,RONCALLI J G,LOSORDO D W.[J].Circulation,2008,118(1):9-16]. [0003] At present, although drug therapy, surgical bypass surgery and ca...

Claims

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Application Information

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IPC IPC(8): A61N7/00G01N15/00C12N13/00
CPCA61N7/00G01N15/00C12N13/00G01N15/01
Inventor 段俊丽周红生郝长宁石一沁李月
Owner 段俊丽
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