Toxoplasma gondii fluorogenic quantitative PCR (polymerase chain reaction) specific primer, kit and detecting method of toxoplasma gondii fluorogenic quantitative PCR specific primer

A fluorescence quantitative and detection method technology, applied in biochemical equipment and methods, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problems of low detection efficiency and insufficient detection sensitivity, and achieve high sensitivity and results. Judging the effect of objective and simple operation
CN105969855AInactive Publication Date: 2016-09-28广州海沥生物科技有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
广州海沥生物科技有限公司
Publication Date
2016-09-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a toxoplasma gondii fluorogenic quantitative PCR specific primer, a kit and a detecting method of the toxoplasma gondii fluorogenic quantitative PCR specific primer. The kit comprises the toxoplasma gondii fluorogenic quantitative PCR specific primer, a DNA extracting solution, a fluorogenic quantitative PCR reacting solution, a quality-controlling reacting solution, Spin Columns CB3 and Collection Tubes 2ml. According to the kit, GRA7 gene is utilized as a genetic marker, and the specific primer is used for optimizing PCR reacting system conditions like reagent concentration and temperature. Thus, the kit can quickly, specifically and flexibly detect whether animals are infected by toxoplasmosis, is convenient to operate and can be applied to diagnosis of various kinds of animal toxoplasmosis.
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Description

technical field

[0001] The invention relates to the technical field of molecular biology diagnosis, in particular to a fluorescent quantitative PCR specific primer and its application detection technology. Background technique

[0002] Toxoplasma gondii is an obligate intracellular parasite. The life cycle of Toxoplasma gondii includes five forms, namely trophozoite (also known as tachyzoite), cyst (which can survive in the tissue for a long time, and can release bradyzoite after rupture), schizont, gametophyte and cyst zygote, The first 3 periods are asexual reproduction, and the last 2 periods are sexual reproduction. The completion of the life cycle of Toxoplasma gondii requires an intermediate host and a final host. Toxoplasma parasitizes in the intermediate host for asexual reproduction, and can only reproduce sexually in the final host.

[0003] Cats and other cats are the ultimate hosts of Toxoplasma gondii. Sexual reproduction of Toxoplasma gondii only develops in ...

Claims

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