Dahurian rose fruit extract and application thereof
A technology of Rosa roxburghii and its extract, which is applied in the field of biochemistry, can solve the problems of increasing the viscosity of cosmetics, unfavorable skin absorption of nutrients, and undisclosed effects of Rosa roxburghii, achieving scavenging of DPPH free radicals and significant anti-aging performance , good whitening and anti-inflammatory effects
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Embodiment 1
[0057] Select 10kg of mature Rosa japonica fruit, wash it twice with deionized water, use a crusher to crush the fruit of Rosa japonica to a particle size of about 60 mesh, add 10 times the volume of deionized water, and extract it in a water bath at 80°C under normal pressure 2h, after the extraction, add 60% ethanol solution, extract in a water bath at 70°C under normal pressure for 2h, after the extraction, mix the extracts twice to obtain a mixed extract 1.
[0058] Select 10kg of mature Rosa cymbidium fruit, wash it twice with deionized water, use a crusher to crush the fruit of Rosa cymbidium to a particle size of 60 mesh, add 15 times the volume of deionized water, and extract it in a water bath at 80°C under normal pressure for 2 hours After the extraction, add 60% ethanol solution, extract in a water bath at 70° C. under normal pressure for 2 hours, and mix the extracts twice to obtain mixed extract II.
[0059] Mix the extract 1 and the extract 2 to obtain the total ...
Embodiment 2
[0078] Choose 10 kg of mature Rosa roxburghii fruit, wash 2 times with deionized water, use a crusher to crush the Rosa rostifolia fruit to a particle size of about 80 mesh, add 10 times the volume of 70% ethanol aqueous solution to extract 5 times in a 65°C water bath, and 5 The obtained filtrates of the second extractions were mixed to obtain a mixed filtrate. The combined filtrate was concentrated by evaporation under reduced pressure to remove ethanol therein. 1M sulfuric acid to adjust the pH value of the concentrated solution to 5, use AB-8 macroporous adsorption resin to absorb the concentrated solution for 2 hours, then use deionized water, 30% ethanol solution and 70% ethanol solution to elute the macroporous adsorption resin at a flow rate of 2BV / h. The mixed eluents were evaporated and concentrated under reduced pressure to remove the ethanol therein to obtain a concentrated solution, which was purified again by using ZTC-5 macroporous adsorption resin. The eluent...
Embodiment 3
[0100] Select 10 kg of mature Rosa cymbra fruit, wash it twice with deionized water, crush the fruit of Rosa cymbidium to a particle size of 60 mesh with a crusher, add 10 times the volume of deionized water, and extract in a water bath at 80°C at 1.2 MPa for 2 hours After the extraction, add 50% methanol solution, extract in a water bath at 70° C. at 1.2 MPa for 2 hours, and mix the extracts twice to obtain a mixed extract.
[0101] Evaporate and concentrate the extract under 5kPa and slight boiling conditions until the extract does not contain methanol to obtain a concentrate. After mixing equal volumes of 1M hydrochloric acid and 1M sulfuric acid, adjust the concentrated solution to pH=5, use AB-8 macroporous adsorption resin to absorb the concentrated solution at 25°C for 2 hours, and then use deionized water, 30% ethanol solution and 70% ethanol solution respectively The macroporous adsorption resin is eluted, and the flow rate of the eluent is 3BV / h. The eluate obtained...
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