Unlock instant, AI-driven research and patent intelligence for your innovation.

A preparation method of fluorescent silica nanoparticles across the blood-brain barrier membrane with different particle sizes

A blood-brain barrier and nanoparticle technology, applied in the field of nano-biomedical materials, can solve the problems of influence of treatment effect, low ability of drug delivery carrier to cross the BBB, low targeting ability, etc., achieve good physical and chemical stability, meet production requirements and application, high biosafety effect

Active Publication Date: 2019-02-05
上海禾澜纳米科技有限公司
View PDF9 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although some achievements have been made in the targeted drug delivery of brain tumors, the current targeted drug delivery to brain tumors still has the problem of low targeting, which is manifested in the low ability of the drug delivery carrier to cross the BBB, which has a greater impact on the therapeutic effect. big impact

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A preparation method of fluorescent silica nanoparticles across the blood-brain barrier membrane with different particle sizes
  • A preparation method of fluorescent silica nanoparticles across the blood-brain barrier membrane with different particle sizes
  • A preparation method of fluorescent silica nanoparticles across the blood-brain barrier membrane with different particle sizes

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Preparation of Fluorescent APTES Precursors

[0029] Take 2.0 mg of 6-carboxyfluorescein and disperse it in 1.0 mL of ethanol, sonicate for a short period of time. After the solution is clarified, add 80 microliters of APTES under the action of a magnetic stirrer and react in the dark for 24 hours to prepare fluorescent APTES. precursor.

[0030] 2. Preparation of FSiNPs

[0031] Dissolve 0.5 mL TEOS and 0.1 mL fluorescent APTES precursor in a mixed solvent of ethanol / water (ethanol: water = 0.5:1) in sequence, then mix with 0.1 mL 25% concentrated ammonia water, and stir for 24 hours. Then, they were washed with ethanol and water three times in sequence, and finally stored in DMF at 4 °C to prepare FSiNPs.

[0032] 3. FSiNPs-PEG 2000 - Preparation of Mal

[0033] Add 1.6 μl of triethylamine to the above DMF solution of FSiNPs, stir well, add 20 mg of NHS-PEG 2000 -Mal, react at room temperature for 2 hours. Wash with DMF and water by centrifugation, and store ...

Embodiment 2

[0038] 1. Preparation of Fluorescent APTES Precursors

[0039] Take 2.0 mg of fluorescein cy3, disperse it in 1.0 mL of ethanol, and sonicate for a short period of time. After the solution is clarified, add 80 microliters of APTES under the action of a magnetic stirrer, and react in the dark for 24 hours to prepare the fluorescent APTES precursor .

[0040] 2. Preparation of FSiNPs

[0041] Dissolve 0.5 mL TEOS and 0.1 mL fluorescent APTES precursor in a mixed solvent of ethanol / water (ethanol: water = 1:1), then mix with 0.2 mL 25% concentrated ammonia water, and stir for 24 hours. Then, they were washed with ethanol and water three times in sequence, and finally stored in DMF at 4 °C to prepare FSiNPs.

[0042] 3. FSiNPs-PEG 2000 - Preparation of Mal

[0043] Add 1.6 μl of triethylamine to the above DMF solution of FSiNPs, stir well, add 20 mg of NHS-PEG 2000-Mal, react at room temperature for 2 hours. Wash with DMF and water by centrifugation, and store in 1.0 mL of 1...

Embodiment 3

[0048] 1. Preparation of Fluorescent APTES Precursors

[0049] Take 2.0 mg of fluorescein cy5.5, disperse it in 1.0 mL of ethanol, sonicate for a short period of time, after the solution is clarified, add 80 microliters of APTES under the action of a magnetic stirrer, and react in the dark for 24 hours to prepare fluorescent APTES precursor.

[0050] 2. Preparation of FSiNPs

[0051] Dissolve 0.5 mL TEOS and 0.1 mL fluorescent APTES precursor in a mixed solvent of ethanol / water (ethanol:water=1.5:1) in sequence, then mix with 0.5 mL 25% concentrated ammonia water, and stir for 24 hours. Then, they were washed with ethanol and water three times in sequence, and finally stored in DMF at 4 °C to prepare FSiNPs.

[0052] 3. FSiNPs-PEG 2000 - Preparation of Mal

[0053] Add 1.6 μl of triethylamine to the above DMF solution of FSiNPs, stir well, add 20 mg of NHS-PEG 2000 -Mal, react at room temperature for 2 hours. Wash with DMF and water by centrifugation, and store in 1.0 mL...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a preparation method for fluorescent blood-brain-barrier-membrane-crossing silica nanoparticles with different particle sizes. The preparation method comprises the following steps: preparation of a fluorescent (3-aminopropyl)triethoxysilane precursor; preparation of fluorescent silica nanoparticles FSiNPs; preparation of FSiNPs-PEG2000-Mal; and preparation of FSiNPs-PEG2000-CDX. The method controls reaction conditions to prepare the fluorescent silica nanoparticles with different particle sizes and selectively connects the surfaces of the fluorescent silica nanoparticles with blood-brain-barrier-membrane-crossing targeting molecules. The fluorescent silica nanoparticles have the characteristics of stable structure, good biocompatibility, strong fluorescence signals, etc. The prepared silica nanoparticles can meet demands of scientific research. The preparation method is simple in process and strong in operability and can further meet demands of production and application.

Description

technical field [0001] The invention specifically relates to a preparation technology for simultaneously connecting fluorescent molecules and blood-brain barrier targeting molecules using silicon dioxide as a medium. The invention belongs to the field of nano biomedical materials. Background technique [0002] According to the World Health Organization (WHO) report in 2008, the incidence of brain tumors is approximately 3.5 / 100,000 globally. In the incidence of systemic tumors, its incidence is second only to those of stomach, uterus, breast and esophageal cancer. In addition, the median survival period of brain tumor patients is very short, only 16 months, and only 3%-10% of patients can live beyond 5 years without treatment. At present, brain tumors, especially gliomas, show infiltrating growth and have unclear borders with surrounding normal brain tissue, making complete surgical resection very difficult, resulting in a high recurrence rate. Chemotherapy can not only k...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A61K47/60A61K47/69A61K49/00
CPCA61K49/0034A61K49/0041A61K49/0043A61K49/005
Inventor 何丹农张鹏王萍金彩虹
Owner 上海禾澜纳米科技有限公司