Method for treating quinoline pollutants in sewage
A treatment method and quinoline technology, applied in water pollutants, filtration treatment, precipitation treatment, etc., can solve the problems of difficult biodegradation and detoxification of quinoline-containing pollutants, achieve high nitrogen and phosphorus removal effect, and improve degradation rate. , the effect of high quinoline removal efficiency
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Embodiment 1
[0022] The treatment of embodiment 1 quinoline compound waste water
[0023] (1) pretreatment: getting and containing quinoline concentration is that 100mg / L sewage is filtered by a conventional filter tank, precipitated by a conventional settling tank, and removes suspended matter in treated sewage;
[0024] (2) Main treatment: add nitrogen oxides (if the nitrogen content is sufficient, you don’t need to add) to the treated sewage that has removed suspended solids, make mixed wastewater, and collect it in the water inlet tank. Among them, carbon and nitrogen The ratio is 8:1, the carbon in the carbon-to-nitrogen ratio comes from the quinoline compound, and the nitrogen comes from the added nitrogen oxide;
[0025] (3) The mixed wastewater is lifted to the upflow biological aerated filter through the inlet pump, and the oxygen supply is aerated for 6 hours;
[0026] (4) The sewage after aeration is transferred to the anoxic zone, and the suspended filler carrying denitrifying...
Embodiment 2
[0028] The acquisition of embodiment 2 quinoline degradation gene PPKL2
[0029] Pseudomonas putida was taken, and quinoline was used as an inducer to induce culture. The induced and uninduced strains were used as a comparison to extract the total protein and perform two-dimensional two-dimensional electrophoresis. Through the comparison, 10 differential expressions were found protein. One of the points with a relatively large expression difference was selected for sequencing. The corresponding amino acid sequence was obtained, and its sequence is shown in Genbank: NP_744642.1. The conventional prokaryotic expression of the gene is carried out, and the specific steps are all conventional techniques. Through the activity measurement, it was found that the protein has a strong quinoline removal effect.
Embodiment 3
[0030] Example 3 The activity of PPKL2 protein mutation is improved
[0031] The corresponding 71D / S, 83S / G, 122R / S, 129Q / T, 149P / G, 189I / R, 207S / G, 224R / P, 239L / S, 248F / G, 263N / E, The 279E / R mutation sites were respectively introduced into the protein shown in Genbank: NP_744642.1, thereby constructing different mutant proteins (can be obtained by referring to the preparation method of the prior art). Different mutant genes were prepared and obtained, and then respectively connected to vectors, and the successfully verified recombinant plasmids were transformed into denitrifying bacteria to obtain genetically engineered bacteria degrading quinoline.
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