Biological treatment agent capable of efficiently degrading kitchen waste and preparation method of biological treatment agent

A technology for food waste and biological treatment, applied in the direction of biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of poor effect and low efficiency, achieve good treatment effect, simple preparation method, and stable treatment effect Effect

Active Publication Date: 2016-10-12
BIOMAX ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the existing biological treatment

Method used

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  • Biological treatment agent capable of efficiently degrading kitchen waste and preparation method of biological treatment agent
  • Biological treatment agent capable of efficiently degrading kitchen waste and preparation method of biological treatment agent
  • Biological treatment agent capable of efficiently degrading kitchen waste and preparation method of biological treatment agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Preparation of biological treatment agent:

[0099] (1) Brevibacterium aureus, Bacillus licheniformis and Bacillus subtilis were inoculated on LB solid medium, Candida glabrata and Pediococcus lactis were inoculated on MRS solid medium. In the bottle, shake culture at 30°C and 130r / min for 24h to form seed liquid.

[0100] Then inoculate the seed liquid into the culture material in the fermenter at a volume ratio of 1:400 for fermentation and production. The basic working parameters of the fermenter are: temperature: 30-35°C, stirring speed: 130r / min, pH=6.2 ~7.2, dissolved oxygen: 30%, tank pressure: 0.04MPa. Stop the fermentation after 24 hours, then centrifuge at 6000r / min for 10 minutes, take the precipitate and dilute it with sterile water to make a bacterial agent. The concentration of viable bacteria in the bacteria agent was detected to be 1×10 9 ~1×10 10 CFU / mL (wherein the fermentation pH of Brevibacterium aureus, Bacillus licheniformis and Bacillus subtil...

example 1

[0104] Biological bacteria agent in example 1 is to the weight loss efficiency test after kitchen waste is processed:

[0105] (1) The composition of kitchen waste: the food left in the canteen, remove the large and hard parts such as plastic or bones;

[0106] (2) Apply the biological treatment agent prepared by the above method to the treatment of kitchen waste. Preheat and run for 6 hours while ventilating and stirring. Add 10kg of food waste to be treated into the processor after pre-operation, mix with biological treatment agent for aerobic fermentation, and ventilate and stir at the same time. The amount of biological treatment agent added is 6.5kg, the aerobic fermentation temperature is 45-55°C, the ventilation is continuous, the motor continues to rotate forward for 5 minutes, stop for 60 seconds, reverse for 5 minutes, stop for 60 seconds, and execute in a cycle. The test lasted for 5 days, adding 10kg every day, feeding once every 24 hours;

[0107] (3) Determina...

Embodiment 2

[0113] In addition to 15 parts of Brevibacterium aureum (Brevibacterium aureum), 25 parts of Bacillus licheniformis (Bacillus licheniformis), 25 parts of Bacillus subtilis (Bacillus subtilis), Candida glabrata ) 20 parts and 15 parts of Pediococcus acidilactici (Pediococcus acidilactici) carry out the liquid inoculant mixing; In step (2), sawdust, bran powder and rice husk are 5:2:3 according to mass ratio; Step (3) according to microbial inoculum and The mass ratio of the carrier is 2:15, except stirring and mixing in the processor, other steps are the same as in Example 1.

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Abstract

The invention provides a biological treatment agent capable of efficiently degrading kitchen waste. The biological treatment agent capable of efficiently degrading kitchen waste comprises a microbial agent and a carrier, and the mass ratio of the microbial agent to the carrier is (1-3):(10-15). By the biological treatment agent capable of efficiently degrading the kitchen waste, brevibacterium flavum, bacillus licheniformis, bacillus subtilis, candida glabrata and pediococcus acidilactici have the synergistic effect on the carrier, the kitchen waste can be degraded efficiently, and decreased amount of fed kitchen waste within 24 hours can be 95% or above. The biological treatment agent is stable in treatment effect and low in cost; the preparation method is simple and convenient; environmental pollution does not exist.

Description

technical field [0001] The invention relates to the technical field of microbial agents, in particular to a biological treatment agent for efficiently degrading kitchen waste, and to a preparation method of the biological treatment agent. Background technique [0002] Kitchen waste mainly comes from food scraps (kitchen waste) and food residues (swill) from catering operations and residents’ lives. There is a large amount of kitchen waste generated by families, schools, canteens and catering industries in my country. If it is not handled properly, it will pollute the environment, spread diseases, and endanger human health. Food waste has the characteristics of high water content (up to 80-95%), high salinity, high organic matter content, perishable odor and breeding of mosquitoes and flies. The main components are water, sugar, protein, fat, salt and oil, etc. . [0003] At present, the treatment methods of food waste are mainly divided into non-biological treatment (incin...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/16C12N11/14B09B3/00C12R1/13C12R1/10C12R1/125C12R1/72C12R1/01
CPCB09B3/00C12N1/16C12N1/20C12N11/14
Inventor 刘立鹤王哲周哲张炳如杜国扬
Owner BIOMAX ECOLOGICAL ENG
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