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Lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture

An aquaculture and lactic acid bacteria technology, applied in the direction of antiviral agents, medical preparations containing active ingredients, pharmaceutical formulas, etc. effect of medicine

Active Publication Date: 2016-10-26
贝嘉美(天津)生物技术研发股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of the above problems, the object of the present invention is to provide a lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture, which effectively solves the disadvantage that antiviral preparations are prone to drug resistance in the existing aquaculture process

Method used

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  • Lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture
  • Lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture
  • Lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021]

[0022] Put the above-mentioned weight of traditional Chinese medicine into a grinder to grind, and pass through a 80-100 mesh sieve to obtain Chinese medicine powder, then add 300mL of water, heat to boiling, and continue boiling for 30 minutes, filter to take the supernatant; continue to add 300mL of water to the filter residue , heated to boiling, and continued to boil for 44 minutes, took the supernatant and mixed with the first supernatant; then continued to add 300mL water to the filter residue, heated to boiling, and continued to boil for 50 minutes, filtered the supernatant and the first two The supernatant of the mixture was mixed to obtain 450 mL of Chinese medicine mixed extract;

[0023] Preparation of MRS liquid medium: tryptone 10.0g, beef extract 10.0g, yeast extract 5.0g, ammonium citrate 2.0g, glucose 20.0g, KH 2 PO 4 2.0g, sodium acetate 5g, Tween-80 1.0mL, magnesium sulfate heptahydrate 0.58g, manganese sulfate 0.25g, KH with a concentration of 0...

Embodiment 2

[0026] The antiviral preparation is prepared with reference to Example 1, the difference is that the Chinese medicine components are as follows:

[0027]

[0028]

[0029] Put the antiviral preparation obtained in Example 1 and Example 2 into carp breeding ponds, and for carp suffering from carp herpes virus disease, calculate the dosage by 1.0mL / kg of fish body weight, and feed the mixed feed continuously for 5 days. up to 88% and 90%.

[0030] The number of effective live bacteria in embodiment one and embodiment two is respectively 6 * 10 10 ,7×10 10 , indicating that the mixed extracts of traditional Chinese medicine can enhance the fermentation of lactic acid bacteria.

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Abstract

A lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture is characterized by being prepared through fermentation of lactic acid bacteria seed solution and well compatible traditional Chinese medicine mixed extract; adopted traditional Chinese medicines include, by mass, 10-20 parts of radix isatidis, 10-20 parts of folium isatidis, 5-10 parts of rheum officinale, 1-5 parts of radix astragali, 3-5 parts of herba houttuyniae, 1-5 parts of abrotani herba, 2-5 parts of herba schizonepetae, 1-5 parts of fructus schizandrae, 4-7 parts of honeysuckle flower, 1-5 parts of fructus forsythia, 5-7 parts of licorice root, 1-5 parts of rhizoma polygoni cuspidate and 1-5 parts of barley malt. The traditional Chinese medicine mixture is obtained based on the traditional Chinese medicine theory and scientific compatibility, the composite effect of syndrome differentiation and treatment of Chinese medicinal formulae is preserved, the extract is obtained through three times of water decoction, effective constituents in the traditional Chinese medicines are reserved to the maximum degree, and the defect of the prior art that traditional Chinese medicines are directly applied to aquaculture and can not be well absorbed is overcome.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation of traditional Chinese medicines, and in particular relates to a lactic acid bacteria traditional Chinese medicine fermentation antiviral preparation for aquaculture. Background technique [0002] Lactic acid bacteria is a general term for a class of spore-free, Gram-positive bacteria. It is a common bacterium in the intestinal tract of animals. It can regulate the nitrification of livestock and poultry to the balance of the flora and improve the immune performance of animals. The microecology of lactic acid bacteria commonly used in the market is currently Most of the preparations use ordinary medium to cultivate lactic acid bacteria, and then exist in the form of liquid or powder. At present, broad-spectrum antiviral western medicines are mostly used in epidemic prevention and treatment of viral diseases. This kind of medicine is easy to produce drug resistance and cause virus mu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/8998A61P31/22A61P31/12
CPCA61K36/195A61K36/282A61K36/315A61K36/355A61K36/481A61K36/484A61K36/538A61K36/57A61K36/634A61K36/704A61K36/708A61K36/78A61K36/79A61K36/855A61K36/8998A61K2236/331A61K2236/39A61K2300/00
Inventor 苏永鹏
Owner 贝嘉美(天津)生物技术研发股份有限公司
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