Preparation method of streptococcus pneumoniae capsular polysaccharide protein conjugate

Abstract

The invention relates to a preparation method of a streptococcus pneumoniae capsular polysaccharide protein conjugate. The preparation method comprises the following steps: dissolving streptococcus pneumoniae capsular polysaccharide in a borate buffer solution, so that a capsular polysaccharide solution is obtained; making the capsular polysaccharide solution contact with CDAP and activating the capsular polysaccharide solution, so that an activated solution is obtained; and making the activated solution contact with carrier protein and promoting bonding, so that the streptococcus pneumoniae capsular polysaccharide protein conjugate is obtained. According to the method provided by the invention, a CDAP method is improved; the streptococcus pneumoniae capsular polysaccharide is dissolved by virtue of the borate buffer solution; the preparation method can avoid an operation of regulating pH value in a reaction and can reduce the use of chemical reagents; and the preparation method is simple in preparation process and high in production efficiency.

Description

technical field [0001] The invention belongs to the field of biomedical chemistry, and in particular relates to a preparation method of Streptococcus pneumoniae capsular polysaccharide protein conjugate. Background technique [0002] Streptococcus pneumoniae is a Gram-positive bacterium that causes considerable morbidity and mortality, causing diseases such as pneumonia, meningitis, otitis media, and bacteremia. Nearly 100 serotypes of Streptococcus pneumoniae have been found, but only 20 of them are pathogenic. Streptococcus pneumoniae capsular polysaccharide is its main virulence determinant because the capsule not only protects the inner surface of the bacterium from complement, but also has weak immunogenicity itself. [0003] In February 2000, the Streptococcus pneumoniae polysaccharide protein-conjugated vaccine Prevnar was first licensed in the United States, and then 10-valent, 11-valent, and 13-valent Streptococcus pneumoniae polysaccharide protein-conjugated vacci...

AI Technical Summary

Problems solved by technology

The key parameters for CDAP activation of polysaccharides are concentration and pH value. The traditional CDAP method uses acid-base reagents to adjust and maintain the pH value in the reaction to complete the activation reaction of polysaccharides. Hexamethylenediamine and adipic dihydrazide are used as spacers. Bind to the carrier protein at a pH value, and maintain the pH value in the binding reaction with an acid-base reagent. During the reaction process, the pH and temperature of the reaction need to be continuously monitored. There are many operating steps, low efficiency, and poor controllability of the reaction.

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