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Kit for culture of adipose-derived stem cells and culture method

A technology of adipose stem cells and a kit, applied in the field of cells, can solve problems such as unsatisfactory effect of culturing adipose stem cells, low cell survival rate and proliferation rate, and the quantity and quality of adipose stem cells cannot meet the requirements of the test, etc.

Active Publication Date: 2016-10-26
BEIJING YULONG SHENGSHI BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to meet the needs of medical research, cultivating a large number of adipose stem cells has become an important research topic, but the reagents and methods selected by the experimenters for isolating, cultivating and preserving adipose stem cells are not appropriate, which often results in the quantity and quality of the cultured adipose stem cells. To meet the requirements of the experiment, manpower and other resources are wasted, so it is of great significance to study a kit specially used for stable large-scale expansion and culture of adipose stem cells
Some kits for culturing fat stem cells are also disclosed in the prior art. For example, CN 104928238A discloses a kit for preparing fat mesenchymal stem cells, including fat preservation solution, fat washing solution, fat decomposition solution, cell Washing solution, cell culture solution, cell digestion solution, and cell cryopreservation solution, but the effect of this kit on culturing adipose stem cells is not ideal, and there are problems such as reagent safety, low stability, low cell survival rate and proliferation rate, etc.

Method used

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  • Kit for culture of adipose-derived stem cells and culture method
  • Kit for culture of adipose-derived stem cells and culture method
  • Kit for culture of adipose-derived stem cells and culture method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] A kit for culturing adipose stem cells, such as figure 1 As shown, it includes a kit body, several reagent bottles 1 and several ice packs, and the kit body includes a box body 2 with an open top, a box cover 3 hinged with the box body 2 and an insertion holder arranged in the box body 2 4. The insertion holder 4 is parallel to the bottom plate of the box body 2, and several jacks for placing the reagent bottle 1 are arranged on it, and a socket with the box body 2 is provided under the insertion holder 4. A hollow partition parallel to the bottom plate, the hollow partition and the box body 2 below form a first cavity, the side wall of the first cavity is provided with a first opening 5 and the first opening 5 Matching first cover plate 6, described box cover 3 is made of box cover upper plate, box cover lower plate 7 parallel with described box cover upper plate, connecting described box cover upper plate and described box cover lower plate 7 Lid side wall 8 is forme...

Embodiment 2

[0052] A kit for culturing adipose stem cells, such as figure 2 As shown, the difference from Example 1 is that a reagent bottle fixing device is provided in the box body 2, and the reagent bottle fixing device includes a fixing cap 11, a first fixing belt 12 symmetrically arranged on both sides of the fixing cap and the The second fixing band 13, the two ends of the first fixing band 12 are fixedly connected with the edge of the fixing cap 11 and the socket 4 respectively, one end of the second fixing band 13 is connected with the fixing cap 11 The edge is fixedly connected, and the other end is connected with the receptacle 4 through a connection structure 14, the connection structure includes a female buckle provided on the second fixing belt 13 and a female button provided on the receptacle 4 and the receptacle 4 Female snaps match male snaps.

Embodiment 3

[0054] A kit for culturing adipose-derived stem cells. The difference from Example 1 is that the tissue preservation solution mainly comprises α-MEM dry powder medium, penicillin, streptomycin, sodium hydroxymethylcellulose, and sodium hypophosphite. Dissolved with water for injection, each L of water for injection dissolves 30 g of the α-MEM dry powder medium and 1 × 10 penicillin respectively. 5 U, belonging streptomycin 0.1g, the sodium hydroxymethylcellulose 8g, the sodium hypophosphite 2g.

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Abstract

The invention provides a kit for culture of adipose-derived stem cells, wherein the kit includes a kit body, a plurality of reagent bottles and a plurality of ice bags; the plurality of reagent bottles are internally filled with a tissue preservation liquid, a basal culture medium, a culture medium additive, a growth factor I, a growth factor II, a tissue decomposition liquid, a cell digestion liquid, a washing liquid and a frozen stock liquid respectively. The kit provided by the invention can separate the adipose-derived stem cells from adipose tissues, and cultures and preserves the adipose-derived stem cells, solves the problems of small separation number and low purity of the adipose-derived stem cells, low culture survival rate, low culture efficiency, nonideal frozen resurrection rate and the like, makes the adipose-derived stem cells subjected to multi-generation amplification and not generate differentiation in an ideal nutrient equilibrium state, provides a rich source for further experimental research with use of the adipose-derived stem cells, and is quite suitable for culture of the adipose-derived stem cells.

Description

technical field [0001] The invention belongs to the field of cell technology, and in particular relates to a kit and a culture method for culturing fat stem cells. Background technique [0002] Stem cells are a type of cells with self-replication and multi-directional differentiation capabilities, which can continuously renew themselves and transform into one or more cells that constitute human tissues or organs under specific conditions. In recent years, stem cell technology has been widely used in clinical trials and treatment of various diseases, and has shown good application prospects, and has gradually become one of the new medical research hotspots in recent years. [0003] There is a type of cell with multidirectional differentiation potential in adipose tissue, that is, adipose stem cell, which has high differentiation potential and can differentiate in multiple directions, and can differentiate into bone, cartilage, muscle, tendon, ligament, nerve, liver, etc. Cel...

Claims

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Application Information

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IPC IPC(8): C12M3/00C12N5/0775A01N1/02
CPCA01N1/0221C12M23/34C12M23/38C12N5/0667C12N2500/30C12N2500/32C12N2500/34C12N2500/38C12N2500/42C12N2501/10C12N2501/90
Inventor 张晓南吴芳春陈虎侍晓云张斌
Owner BEIJING YULONG SHENGSHI BIOTECH CO LTD
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