Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Space kocuria kristinae strain LCT-H5

A technology of LCT-H5 and bacteria strains, which is applied in the direction of bacteria, microorganisms, biochemical equipment and methods, and can solve the problem of difficult access to genetic information

Inactive Publication Date: 2016-10-26
GENERAL HOSPITAL OF PLA
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Early understanding of many environmental microorganisms came only from the study of 16S rRNA, some important genetic information is difficult to obtain

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Space kocuria kristinae strain LCT-H5
  • Space kocuria kristinae strain LCT-H5
  • Space kocuria kristinae strain LCT-H5

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Example 1: Cultivation of strain LCT-H5.

[0016] Strain LCT-H5 was isolated from the return cabin of the "Shenzhou 9" spacecraft. The strains were cultured by standing or shaking in a 37°C incubator or shaker, the liquid medium was brain heart infusion medium (BHI, Oxiod), and the formula was 37 g / L BHI; the solid medium was MH plate ( figure 1 ).

Embodiment 2

[0017] Example 2: Identification of strain LCT-H5 and construction of phylogenetic tree.

[0018] Strain LCT-H5 was inoculated into BHI liquid medium, cultured at 37°C at 180 rpm overnight, centrifuged at 6000 rpm for 5 min to collect bacterial cells, and the supernatant was discarded. The genomic DNA of the collected bacterial cells was extracted using the genomic DNA extraction kit of Qiagen (Code No. 51304, see the product manual for detailed steps). Using the genomic DNA of LCT-H5 as a template, the 16s rRNA fragment was amplified and sequenced using the bacterial 16s rRNA universal sequencing primers 27F (5'-AGAGTTTGATCCTGGCTCAG-3') and 1492R (5'-TACGGCTACCTTGTTACGACTT-3'), and the bases were obtained. sequence. Multiple sequence alignments were performed with CLUSTALW (http: / / www.ebi.ac.uk / Tools / msa / clustalw2 / ) and NJ phylogenetic trees were constructed with MEGA 5.0 ( figure 2 ).

Embodiment 3

[0019] Example 3: Gram staining of strain LCT-H5.

[0020] LCT-H5 cells were stained with Gram staining kit (Code No. G1060, Solarbio). The bacteria were cultured in BHI liquid medium overnight, and the bacteria were dropped in the center of the glass slide and warmed slightly on an alcohol lamp to dry quickly. Crystal violet and sand yellow staining solution can stain and decolorize the dried bacteria once. For detailed steps, please refer to the product manual. After the dyeing was completed and dried, the dyeing condition was observed under a light microscope ( image 3 ).

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a space kocuria kristinae strain LCT-H5 serving as a downstream strain obtained by separation from a Shenzhou Nine return capsule. The space kocuria kristinae strain LCT-H5 belongs to gram-positive coccus, adopts a quadruple arrangement or adopts a paired and scattered arrangement; multiple carbon sources such as glucose, d-trehalose anhydrous, mannitol and saccharose can be utilized. The strain is subjected to 16S-rRNA sequencing and is identified as a member of kocuria kristinae. Whole genome sequencing is performed on the coccus to find that the genome size of the coccus is 2.4Mbp, the genome comprises 1991 coding sequences and 101RNA (Ribonucleic Acid) genes, and 22 groups of COG (Cluster of Orthologous Groups) functional classifications are totally annotated by COG database comparison. The patent provides certain help for researching the distribution of kinds of microorganisms in a closed aircraft.

Description

technical field [0001] The present invention relates to a coxa cruzi LCT-H5 strain isolated in a space environment, its biological characteristics and whole genome sequencing. Background technique [0002] From the successful moon landing to the future exploration of Mars, the activities of human exploration of outer space are not only more and more frequent, but their scope is also increasing. Microorganisms are part of the natural environment, and many bacteria also exist in human skin, oral cavity, nasopharynx and gastrointestinal tract. Some studies have found that Salmonella typhimurium cultured under simulated microgravity conditions will increase the virulence of infection in mice, and the immunity of astronauts will decline to varying degrees in the space environment, which may easily lead to opportunistic infections. At present, a variety of bacteria including Klebsiella pneumoniae, Staphylococcus aureus, Pseudomonas aeruginosa, Escherichia coli, Serratia, Bacillus...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12R1/265
CPCC12N1/205C12R2001/265
Inventor 刘长庭张学林潘磊姜学革方向群郭英华王俊峰周宏徐绸刘岩余昳
Owner GENERAL HOSPITAL OF PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com