43 results about "Gram Positive Coccus" patented technology

A novel strain of lactic acid bacteria was found to be heat resistant and able to grow in a sulfur-limiting medium (SLM) containing a high concentration of sodium selenite. The microorganism is a non-spore forming and Gram-positive coccus, which is identified with >90% confidence using the API biochemical and sugar fermentation tests, ribotyoing and 16S rRNA sequencing as Pediococcus pentosaceus SP80. In the current study, P. pentosaceus SP80 grown on SLM containing 250 ppm sodium selenite produced both organic and inorganic forms of selenium. These selenium compounds can be separated using an anion exchange chromatography technique. The concentrations of selenium detected in the organic and inorganic fractions were 4.34 and 21.7 ppm, respectively. Selenium-enriched bacteria are useful as a source of selenium for supplementing the diets of animals and humans. Animals fed efficacious amounts of the selenium-enriched bacteria show improved feed conversion rates and higher levels of glutathione peroxidase (GPx) activity in heart, kidney and liver tissues indicating an increased absorption and retention of selenium over control diets.

The invention belongs to the technical field of biotechnology and relates to the sequences of five oligonucleotide aptamers for specifically identifying staphylococcus aurei and use thereof. The fivesinge-chain DNA oligonucleotide aptamers for specifically identifying the staphylococcus aureus are obtained by a bacterial subtraction SELEX technology. After FITC is marked, the five aptamers are proved by fluorescent staining to be capable of specifically identifying the strain of the staphylococcus aurei. After being used in combination, the five ligands can improve sensibility in identification of target bacteria and the ratios of detected different staphylococcus aureus strains and the detected staphylococcus aurei in different growth states. Therefore, the sequences of the five aptamershave wide application prospects in quick detection of the staphylococcus aurei in samples including suspected terrorist attack biological agents and identification of the staphylococcus aurei and other gram-positive cocci.

An extraction method of the fly intestinal microorganism total DNA (deoxyribonucleic acid) relates to the technical field of experiment of molecular biology. The phenol-chloroform method is the traditional method for extracting bacterial DNAs, but the method is generally used in the DNA extraction of pure bacteria cultured in the laboratory. As the matrix ingredients and microorganisms of the environment sample are more complicated, the phenol-chloroform method and the kit method have the defects such as low cell lysis rate and large DNA loss when used for extracting total DNA; and the residual enzyme inhibitor in the extract can affect the subsequent polymerase chain reaction (PCR) amplification effect and the enzymatic reaction effect. Therefore, the extraction method is improved in the aspects such as the sample pretreatment, the operating step and the reagent solution; and in the method, the sodium-tris-EDTA (STE) buffer is used to pretreat the sample and lysozyme is used to ensure the completely splitting of Gram-positive cocci. When the fly intestinal microorganism total DNA extracted by the method is used for PCR amplification and 16S rRNA (ribosomal ribonucleic acid) library construction, the effect is better than that of domestic and import kits. The operating steps of the method are simple, and the pollution of the operating process to the sample and the damage of the operating process to DNA molecules can be effectively reduced.

The invention discloses protamine compounded preparation, a preparation method and application thereof. The protamine compounded preparation of the invention can be singly prepared with the protamine and chitosan, or prepared by compounding one or mixture of over two of glycine, sodium acetate, undecynol monoglyceride, acetic acid, absolute alcohol, lysostaphin, lysozyme, anti-bacterial peptide and sterilized distilled water. The method for preparing the protamine compounded preparation comprises the following steps: accurately weighting each component according to rate in aseptic or clean workshops; putting in clean glass or stainless steel, and uniformly stirring; and sealing in sterile containers. The product not only effectively kills the gram-positive cocci, such as staphyloccocus aureus rosenbach, monilia albican, neisseria gonorrhoeae, streptococcus, anaerobic bacteria, pseudomonas aeruginosa, escherichia coli, pneumococcus, D enterococcus, staphylococcus tetragenus, listeria monocytogenes, stenotrophomonas maltophilia and stomach helicobacter pylori, but also kills the gram-negative bacteria.

3-(1-Aminocycloalkyl)pyrrolidinyl-substituted-6-dehalodeno(hydrogen-substituted)quinolon carboxylic acid derivatives having specific substituents as represented by the following formula (I), its salts, and hydrates thereof exhibit a broad and potent antibacterial activity on gram-negative and gram-positive bacteria, in particular, resistant bacteria typified by gram-positive cocci, including MRSA, PRSP and VRE. Thus these compounds are usable as drugs.

The present invention is one kind of natural cell growth regulating factor X and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding kudzu vine juice into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor X has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified histiocyte, regenerating capillary vessel, so that it can treat sclerotic arthritis and titanic rachitis and shape normal joint and skeleton.

The invention provides a separation and identification method of a microorganism causing mildew of fermented chili, and belongs to the technical field of microorganisms. The method is characterized inthat the microorganism in the 'mildewed' fermented chili is separated to obtain two main 'mildewing' bacteria. The two main 'mildewing' bacteria are subjected to inversed grafting to the sterilized fermented chili; the strain separated from a PDA culture medium causes 'mildewing'; the separated strain is subjected to ITS sequence analysis, and the result verifies that the strain causing 'mildewing' of the fermented chili is pichiakudriavzevii. The strain separated from an NA culture medium cannot cause the 'mildewing' of the fermented chili, and the strain is primarily identified as Gram-positive cocci by inspection of a gram stain microscope.

The present invention is one kind of natural cell growth regulating factor I and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding alanine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor I has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified pancreatic histiocyte, regenerating capillary vessel, so that it can rejuvenate and delay senility.

The invention relates to a method for enriching bacteria, belongs to the technical field of biology, and particularly relates to a method for enriching staphylococcus aureus in milk. The method for enriching the staphylococcus aureus in the milk comprises the following steps of A, collecting a raw milk sample; B, preparing a selective culture medium for staphylococcus aureus enrichment; C, performing separation culture of the staphylococcus aureus, and performing streak inoculation of the collected milk sample to the selective culture medium; D, performing Gram staining microscopy, and extracting a suspected bacterial colony in the selective culture medium, wherein the extracted staphylococcus aureus is Gram-positive cocci; and E, performing enrichment culture of the staphylococcus aureus, transferring the extracted Gram-positive cocci in the step D to a nutrient agar culture medium for performing pure culture, performing enrichment on the staphylococcus aureus, and obtaining the enriched staphylococcus aureus. An economic, quick and specific method for enriching the staphylococcus aureus in the milk is established; and by adopting the method, the staphylococcus aureus in the milk can be quickly, simply and conveniently separated, so that a reliable basis is provided for subsequent identification of the staphylococcus aureus.

The present invention is one kind of natural cell growth regulating factor F and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding alanine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor F has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified pancreatic histiocyte, regenerating capillary vessel, so that it can repair and reform skin and soft tissue.

The present invention is one kind of natural cell growth regulating factor D and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding isoleucine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor D has the functions of stimulating and strengthening immunity, promoting the amplification of capillary vessel in fracture and pathological change part and repairing and reforming pathological change histiocyte.

The present invention is natural cell regulating factor J and its preparation process. It is prepared through screening Gram-positive coccus, Gram-negative coccus or bacillus, freezing preservation of strain, conventional culture of the strain at 4-38 deg.c, filtering to filter out harmful thallus while leafing beneficial thallus or extracting natural gene, adding serine into the natural gene, safety inspection and forming into oral liquid or freeze dried powder. The natural cell regulating factor J product has serine content the same as that of natural cell regulating factor J. It has the functions of activating and strengthening immune, strengthening TC, repairing and regenerating cell tissue, vessel and local tissue cell, etc.

The invention discloses solid yogurt rich in Omega-3 and probiotics and a preparation method thereof, and relates to food and a preparation method thereof. The solid yogurt comprises the following components, by weight: 30-50 parts of yogurt, 20-40 parts of an ingredient, 10-15 parts of sugar, 5-10 parts of chia seed oil, 3-5 parts of probiotics, and 1-3 parts of an emulsifier. The ingredient is one of nuts or dried fruits. The sugar is one or more of honey or white sugar. The chia seed oil is oil extracted from chia seeds. The probiotics are one or more of bifidobacteria, lactobacilli and Gram-positive cocci. The preparation method includes the following steps: fully mixing the yogurt, the chia seed oil and the emulsifier at 20-50 DEG C; adding the white sugar and the probiotics, and fully mixing the components; adding the ingredient, and stirring and mixing the materials well; and freeze-drying the mixture to obtain the solid yogurt. The preparation method makes the yogurt maintain asolid state under normal temperature conditions through a reasonable formula and a unique process. Therefore, the solid yogurt products have unique taste and rich nutrition, and are especially rich in the Omega-3 and the probiotics.

The invention provides a feed additive for preventing and controlling digestive tract diseases of black goats and relates to the field of breeding feed. The feed additive is prepared from raw materials in parts by weight as follows: 5-12 parts of rhizoma dryopteris crassirhizomae, 10-15 parts of fructus piperis longi, 15-25 parts of fructus tsaoko, 3-6 parts of radix chimonanthus, 6-10 parts of pumpkin seeds, 5-10 parts of herba schizonepetae, 2-6 parts of coriander, 3-5 parts of leatherleaf mahonia, 10-15 parts of a digestive formula and 20-25 parts of a nutritional agent. According to the feed additive, digestive ability of the gastrointestinal system of the black goats can be promoted, digestion and absorption for feed and other food are enhanced, meanwhile, growth of microflorae such as Welch's bacillus, streptococcus, helicobacter pylori and the like easily causing digestive tract diseases can be inhibited, growth of lactobacillus, bifidobacteria and Gram-positive cocci is facilitated, so that gastrointestinal functions are protected, diarrhea, allergy and tumor are prevented, incidence of yellow scour and white diarrhea is reduced, and healthy growth of the black goats is guaranteed.

The present invention is one kind of natural cell growth regulating factor Q and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding lysine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor Q has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified histiocyte, regenerating capillary vessel, so that it can repair and reform kidney histocyte.

The present invention is one kind of natural cell growth regulating factor W and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding fleeceflower root extractive into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor W has the functions of stimulating and strengthening immunity, strengthening TC, promoting the mature of leukocyte, repairing various blood cell components and reforming normal blood form and providing whole body with blood normally.

The present invention is one kind of natural cell growth regulating factor H and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding tyrosine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor H has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified pancreatic histiocyte, regenerating capillary vessel, so that it can rejuvenate prostate and delay senility.

The present invention is one kind of natural cell growth regulating factor K and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding theronine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor K has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified histiocyte, regenerating capillary vessel, so that it can rejuvenate cardiac muscle cell and delay senility.

The present invention is one kind of natural cell growth regulating factor V and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding alanine into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor V has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified pancreatic histiocyte, regenerating capillary vessel, so that it can repair and reform gastric and duodenal mucous membrane tissues.

The invention provides a compound preparation prepared from clindamycin and cimetidine and a preparation method of the compound preparation. The clindamycin and cimetidine compound preparation comprises the active ingredients of clindamycin and cimetidine in a ratio of (0.075-1.2): (0.01-0.4). The preparation method comprises the following steps: taking the raw material medicines in corresponding parts, and adding proper auxiliary materials according to a corresponding preparation preparation method of pharmaceutics, so as to respectively prepare dosage forms such as granules, dry suspension, tablets, capsules, injection and the like. The action mechanism is that synthesis of bacterial protein is inhibited to generate an antibacterial effect, and the compound is mainly used for infection caused by gram-positive cocci and anaerobic bacteria. Cimetidine is a liver drug enzyme inhibitor and can delay metabolism of the combined drug and improve the blood concentration of the combined drug so as to improve the curative effect of the combined drug; meanwhile, cimetidine can also inhibit gastric acid secretion so as to relieve the stimulation of clindamycin to the stomach. The compound preparation prepared from the two medicines is used for treating infection caused by sensitive bacteria, and has the advantages of good effect, low side effect, safety in medication, convenience in taking and good application effect.

The present invention is natural cell regulating factor M and its preparation process. It is prepared through screening Gram-positive coccus, Gram-negative coccus or bacillus, freezing preservation of strain, conventional culture of the strain at 4-38 deg.c, filtering to filter out harmful thallus while leafing beneficial thallus or extracting natural gene, adding glutamine into the natural gene,safety inspection and forming into oral liquid or freeze dried powder. The natural cell regulating factor M product has glutamine content the same as that of natural cell regulating factor M. It has the functions of activating and strengthening immune, strengthening TC, repairing and regenerating cell tissue, vessel and liver and kidney tissue cell, etc.

The present invention is one kind of natural cell growth regulating factor E and its preparation process. The preparation process includes screening Gram-positive coccus or bacillus and Gram-negative coccus or bacillus, freeze preserving coccus or bacillus strain, conventional culture of the coccus or bacillus strain at 4-38 deg.c, once filtering to eliminate harmful bacteria while leaving beneficial thallus segment, extracting natural gene, adding proline into the natural gene, safety test, preparing into solution or powder, and sealing. The cell growth regulating factor E has the functions of stimulating and strengthening immunity, strengthening TC, repairing and reforming necrotic, degenerative, atrophic, fibrillated and calcified pancreatic histiocyte, regenerating capillary vessel, so that it can rejuvenate, delay senility and make joints flexible.