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A material for promoting osteogenic differentiation of mesenchymal stem cells and its preparation method and application

A stem cell and osteogenic differentiation technology, applied in biochemical equipment and methods, animal cells, vertebrate cells, etc., can solve the problem that the electrical characteristics cannot match the natural bone healing process, etc., to promote adhesion and proliferation, strong controllability , the effect of good affinity

Active Publication Date: 2019-07-02
PEKING UNIV SCHOOL OF STOMATOLOGY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The present invention aims to solve the technical problems that the electrical properties of the existing electroactive materials cannot match the natural bone healing process. Based on the importance of the damage potential for bone repair and reconstruction, the damage potential model is constructed by using bismuth ferrite (BFO) with high polarization intensity. , Establish a material for simulating injury potential to promote osteogenic differentiation of mesenchymal stem cells, its preparation method and application

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  • A material for promoting osteogenic differentiation of mesenchymal stem cells and its preparation method and application
  • A material for promoting osteogenic differentiation of mesenchymal stem cells and its preparation method and application
  • A material for promoting osteogenic differentiation of mesenchymal stem cells and its preparation method and application

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Embodiment 1

[0027] (1) Prepare an electroactive bismuth ferrite (BFO) thin film material model to simulate the damage potential. Strontium titanate (STO) was used as the substrate, and the BFO epitaxial film with simulated damage potential was prepared by pulsed laser deposition (PLD). The growth temperature is 700°C, the laser frequency for growing the interlayer strontium ruthenate is 2HZ; the laser energy is 70mj; the oxygen partial pressure is 13pa; the growth time is 20min. The laser frequency for growing BFO is 5HZ; the laser energy is 75mj; the oxygen partial pressure is 18pa; the growth time is 30min.

[0028] (2) Expansion and culture of bone marrow-derived mesenchymal stem cells: using mesenchymal stem cell medium without osteoinductive factors (mesenchymal stem cell basal medium+10% fetal bovine serum+100IU / mL penicillin-streptomycin, All were purchased from Saiye Biotechnology Co., Ltd., the same below). After recovering, MSCs were grown adherently in T75 culture flasks, dig...

Embodiment 2

[0034] (1) Preparation of electroactive material bismuth ferrite (BFO) thin film to simulate damage potential. Using STO as the substrate, BFO epitaxial film with damage potential simulated by pulsed laser deposition (PLD). The growth temperature is 300°C, the laser frequency for growing the middle layer SRO is 5HZ; the laser energy is 80mj; the oxygen partial pressure is 15pa; the growth time is 24min. When growing BFO, the laser frequency is 5HZ; the laser energy is 80mj; the oxygen partial pressure is 15pa; the growth time is 90min.

[0035] (2) Expansion culture of bone marrow-derived mesenchymal stem cells: Using mesenchymal stem cell culture medium without osteoinductive factors, MSCs cells were resuscitated and grown adherently in T75 culture flasks, and digested with 0.25% trypsin every 3 days and dried pass on.

[0036] (3) Induce MSCs to differentiate into osteoblasts: Take 3-6 generations of MSCs and inoculate them on the surface of BFO with different polarization...

Embodiment 3

[0041](1) Preparation of electroactive material bismuth ferrite (BFO) thin film to simulate damage potential. Using STO as the substrate, BFO epitaxial film with damage potential simulated by pulsed laser deposition (PLD). The growth temperature is 500°C, the laser frequency for growing the middle layer SRO is 5HZ; the laser energy is 95mj; the oxygen partial pressure is 20pa; the growth time is 2min. When growing BFO, the laser frequency is 5HZ; the laser energy is 105mj; the oxygen partial pressure is 20pa; the growth time is 10min.

[0042] (2) Expansion culture of bone marrow-derived mesenchymal stem cells: Using mesenchymal stem cell culture medium without osteoinductive factors, MSCs cells were resuscitated and grown adherently in T75 culture flasks, and digested with 0.25% trypsin every 3 days and dried pass on.

[0043] (3) Induce MSCs to differentiate into osteoblasts: Take 3-6 generations of MSCs and inoculate them on the surface of BFO with different polarization ...

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Abstract

The invention relates to a material for promoting mesenchymal stem cell osteoblastic differentiation and solves the technical problem that electrical characteristics of existing electrical active materials cannot match with natural bone healing process. The material is a thin film using strontium titanate as a substrate and strontium ruthenate as a middle layer and having bismuth ferrite deposited on the surface. The invention further provides and a preparation method and application of the material. The material can be used in the technical field of promoting mesenchymal stem cell osteoblastic differentiation.

Description

technical field [0001] The invention relates to the technical field of promoting osteogenic differentiation of mesenchymal stem cells, in particular to a method for promoting osteogenic differentiation of mesenchymal stem cells, a preparation method and application thereof. Background technique [0002] Stem cells are important seed cells for regenerative medicine due to their high self-renewal ability and multi-directional differentiation potential. Simulating the complex microenvironment of stem cells in vivo, regulating the fate of stem cells, and realizing their directional differentiation in vitro have become the research hotspots of regenerative medicine. A large number of studies have confirmed that growth factors, topological structure, elastic modulus, etc. can individually or synergistically affect stem cell differentiation. In recent years, the surface charge of biomaterials has gradually become another important factor affecting the behavior of stem cells. [0...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/077C12N5/0775C23C14/28C23C14/08
CPCC12N5/0654C12N2506/1353C12N2506/1384C12N2533/10C23C14/08C23C14/28
Inventor 邓旭亮刘云卫彦张学慧张金星
Owner PEKING UNIV SCHOOL OF STOMATOLOGY
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