Preparation method of marrow fluid smear

A technique for bone marrow fluid and bone marrow, applied in the field of preparation of bone marrow fluid smears, can solve the problems of uneven coloring of bone marrow smears, unfavorable bone marrow cell examination, too deep or too light staining, etc. Vivid, good contrast effect

Inactive Publication Date: 2016-10-26
SICHUAN KINGMED DIAGNOSTICS CENT
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the dyeing method of this method adopts the dripping method, and the dyestuff is easily deposited on the glass slide during the dripping, and there are more dye particles to exist, and the ratio of the buffer solution and the dyeing solution is difficult

Method used

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  • Preparation method of marrow fluid smear
  • Preparation method of marrow fluid smear
  • Preparation method of marrow fluid smear

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Experimental program
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Effect test

Embodiment 1

[0031] Embodiment 1, the preparation of a kind of bone marrow fluid smear

[0032] S1 Extract the bone marrow fluid, add 0.2% of the volume of the bone marrow fluid, and react for 35 seconds with an anticoagulant at a concentration of 2 mg / ml. The anticoagulant consists of heparin and dipotassium edetate in a weight ratio of 1:2. Obtain anticoagulated bone marrow fluid;

[0033] S2 Take 5 μL of the anticoagulated bone marrow liquid obtained in step S1 and drop it onto one end of the glass slide, and smear the bone marrow liquid into a thin slice with a push slide, the angle between the push slide and the slide glass is 35°, and a smear is obtained;

[0034] S3 arranges the smears obtained in step S2 on the slide rack, puts the slide rack into the staining vat 1 and fixes it for 1 min, and the staining vat 1 is a staining liquid, and the staining liquid is 1 g of Wright's dye, Add 0.3g of Giemsa dye powder to 500ml of methanol, then add 10ml of glycerin and mix well, place the...

Embodiment 2

[0037] Embodiment 2, the preparation of a kind of bone marrow fluid smear

[0038] S1 Extract the bone marrow fluid, add 0.2% of the volume of the bone marrow fluid, and react for 45 seconds with an anticoagulant with a concentration of 3 mg / ml. The anticoagulant consists of heparin and dipotassium edetate in a weight ratio of 1:3. Obtain anticoagulated bone marrow fluid;

[0039] S2 Take 6 μL of the anticoagulated bone marrow liquid obtained in step S1 and drop it on one end of the glass slide, and use a push slide to smear the bone marrow liquid into a thin slice, and the angle between the push slide and the slide glass is 35° to obtain a smear;

[0040] S3 arranges the smears obtained in step S2 on the slide rack, puts the slide rack into the staining vat 1 and fixes it for 2 min, and the staining vat 1 is a staining liquid, and the staining liquid is 1 g of Wright's dye, Add 0.3g of Giemsa dye powder to 500ml of methanol, then add 10ml of glycerin and mix well, place the ...

Embodiment 3

[0043] Embodiment 3, the preparation of a kind of bone marrow fluid smear

[0044] S1 Extract the bone marrow fluid, add 0.3% of the volume of the bone marrow fluid, and react for 55 seconds with an anticoagulant at a concentration of 4 mg / ml. The anticoagulant consists of heparin and dipotassium edetate in a weight ratio of 1:4. Obtain anticoagulated bone marrow fluid;

[0045] S2 Take 7 μL of the anticoagulated bone marrow liquid obtained in step S1 and drop it on one end of the glass slide, and use a push slide to smear the bone marrow liquid into a thin slice, and the angle between the push slide and the slide glass is 35° to obtain a smear;

[0046] S3 arranges the smears obtained in step S2 on the slide rack, puts the slide rack into staining vat 1 and fixes it for 3 min, and the staining vat 1 is a staining liquid, and the staining liquid is 1 g of Wright's dye, Add 0.3g of Giemsa dye powder to 500ml of methanol, then add 10ml of glycerin to mix well, place the prepare...

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Abstract

The invention belongs to the field of marrow cell examination, and specifically relates to a preparation method of a marrow fluid smear. The preparation method comprises steps of extracting marrow fluids, adding an anti-coagulant, smearing the marrow fluids, and carrying out dyeing. The provided preparation method had the advantages that the morphology of marrow cells can be effectively maintained, the marrow cells can be distributed more evenly, the classification of marrow cells becomes easier; and the marrow cell examination becomes more accurate. At the same time, the operation is simple and convenient, the quality is easy to control, moreover, the prepared smear is uniformly dyed; the dyed cells are bright and symmetrical, the structure is clear; the color and transparency of cytoplasm can represent the corresponding characteristics of various cells in each phase; the contrast ratio of cytoplasm and cell nucleus dyeing is good, and the provided marrow fluid smear preparation method is ideal.

Description

technical field [0001] The invention belongs to the field of bone marrow cytology examination, and in particular relates to a preparation method of a bone marrow fluid smear. Background technique [0002] With the continuous development of science and the interpenetration among disciplines, the field of hematology has been continuously expanded, and the detection methods have become more and more abundant. Cell biochemistry, immunology, genetics, cell dynamics, and electron microscopy have brought morphological examinations to the molecular level, and in daily clinical work, cell morphological examinations under light microscopy are the preferred and essential means. Bone marrow examination can be used for the diagnosis of hematopoietic system diseases, such as the differential diagnosis of leukemia, various anemias, multiple myeloma and the diagnosis of increased or decreased platelets; bone marrow examination can also be used for some infectious diseases, such as For infe...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/2813G01N1/30
Inventor 王茂书欧阳小峰彭宇
Owner SICHUAN KINGMED DIAGNOSTICS CENT
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