A kind of dehydrogenase electrode and its application
A dehydrogenase and electrode technology, which is applied in the field of dehydrogenase electrodes, can solve the problems of lowering the detection limit and failing to meet the detection of glutamic acid, and achieves the effects of lowering the detection limit, simple preparation method, and good reproducibility
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Embodiment 1
[0032] 1) Activation: place the glassy carbon electrode in a 0.1M, pH 7.4 phosphate buffer solution and activate it for 20 minutes by cyclic voltammetry at an activation potential of -1.6V to +2.7V to prepare the activated glassy carbon electrode;
[0033] 2) Adsorption: placing the activated glassy carbon electrode prepared in step 1) in a 1 mM methylene green solution for 2 hours to obtain a glassy carbon electrode adsorbed with a methylene green catalyst;
[0034] 3) Enzyme immobilization: The glassy carbon electrode adsorbing methylene green prepared in step 2) was drip-coated with glutamate dehydrogenase, ascorbate oxidase and cross-linking agent, cross-linked at room temperature and then fixed in a refrigerator at 4°C. A dehydrogenase electrode (or an electrochemical sensor) is prepared.
Embodiment 2
[0036] The difference between this example and Example 1 is that the methylene green solution is replaced by the methylene blue solution.
Embodiment 3
[0038] The difference between this example and Example 1 is that the glassy carbon electrode adsorbed methylene green prepared in step 2) was drip-coated with glutamate dehydrogenase, alanine aminotransferase, ascorbate oxidase and a cross-linking agent.
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