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Rapid propagation method for Zanthoxylum avicennae suspension cells and regeneration plants

A technology for suspending cells and regenerating plants, which is applied in the biological field to achieve the effects of high survival rate, low energy consumption and high proliferation rate

Inactive Publication Date: 2016-11-09
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Distributed in sparse forests in Fujian, Guangdong, and Guangxi, it has the effects of dispelling wind and dampness, promoting blood circulation and relieving pain. The root can treat icteric hepatitis, nephritis edema, and rheumatoid arthritis; the fruit is used to treat stomach pain and abdominal pain; the leaves are mainly used for traumatic injuries Injury, lumbar muscle strain, mastitis, furunculosis, no relevant reports on the reproductive technology of Bougainvillea have been found so far

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the leaves of Bougainvillea, soak them in washing powder for 2 minutes, wash them with running water for 20 minutes, treat them with 2.5% sodium hypochlorite on an ultra-clean workbench for 5 minutes, treat them with 1% bromine water for 5 minutes, rinse them with sterile water 5 times, and pick them up. The formulation of the culture medium is NH+IAA0.1mg / L+ZT2mg / L induction medium for callus induction culture, adding 30g / L sucrose and 5.5g / L agar, and the induced callus is put into the medium without adding agar In the above-mentioned liquid medium formula, 110r / min rotary shaker was cultivated for 15 days to establish a suspension cell line with good dispersion, uniformity and rapid growth. It was carried out under dark conditions, with a temperature of 28±1°C and a pH of 5.8. The culture conditions were at The inoculum amount in 40mL culture medium is 0.5g, subculture once every 18 days, retain 1 / 3 volume of the old culture medium during subculture, and transfer...

Embodiment 2

[0011] Take the leaves of Bougainvillea, soak them in washing powder for 2 minutes, wash them with running water for 20 minutes, treat them with 2.5% sodium hypochlorite on an ultra-clean workbench for 5 minutes, treat them with 1% bromine water for 5 minutes, rinse them with sterile water 5 times, and pick them up. The formulation of the culture medium is NH+IAA0.1mg / L+ZT2mg / L induction medium for callus induction culture, adding 30g / L sucrose and 5.5g / L agar, and the induced callus is put into the medium without adding agar In the above-mentioned liquid medium formula, 110r / min rotary shaker was cultivated for 15 days to establish a suspension cell line with good dispersion, uniformity and rapid growth. It was carried out under dark conditions, with a temperature of 28±1°C and a pH of 5.8. The culture conditions were at The inoculum amount in 40mL culture medium is 0.5g, subculture once every 18 days, retain 1 / 3 volume of the old culture medium during subculture, and transfer...

Embodiment 3

[0013] Take the leaves of Bougainvillea, soak them in washing powder for 2 minutes, wash them with running water for 20 minutes, treat them with 2.5% sodium hypochlorite on an ultra-clean workbench for 5 minutes, treat them with 1% bromine water for 5 minutes, rinse them with sterile water 5 times, and pick them up. The formulation of the culture medium is NH+IAA0.1mg / L+ZT2mg / L induction medium for callus induction culture, adding 30g / L sucrose and 5.5g / L agar, and the induced callus is put into the medium without adding agar In the above-mentioned liquid medium formula, 110r / min rotary shaker was cultivated for 15 days to establish a suspension cell line with good dispersion, uniformity and rapid growth. It was carried out under dark conditions, with a temperature of 28±1°C and a pH of 5.8. The culture conditions were at The inoculum amount in 40mL culture medium is 0.5g, subculture once every 18 days, retain 1 / 3 volume of the old culture medium during subculture, and transfer...

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PUM

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Abstract

The invention relates to a rapid propagation method for Zanthoxylum avicennae suspension cells and regeneration plants. The method includes the steps of leaf disinfection, callus induction, suspension cell culture, plant regeneration, acclimatization and transplanting, and the like. By adopting the method for tissue culture of Zanthoxylum avicennae, constraints from seasons, the climate, the region and other factors are avoided, land is saved, the speed is high, the quality is high, and intensive management and factory production are promoted.

Description

technical field [0001] The invention relates to a method for rapid propagation of bougainvillea tissue culture, and belongs to the field of biotechnology. Background technique [0002] 簕欓, Zanthoxylum avicennae , Rutaceae, also known as Zanthoxylum bungeanum, Eagle Bubo, evergreen shrub or tree, up to 12 meters high, with prickles on the trunk and branches, prickles triangular, reddish brown, straight or slightly upward, singular pinnate compound leaves , row of students; leaflets 7-23, papery to leathery, obround, obovate-oblong or asymmetric rhomboid, 2-6 cm long, entire or with inconspicuous serrations above the middle, Glabrous, shiny above, corymb-shaped panicles, terminal; flowers are small and numerous, light blue; female flowers are slightly larger than male flowers, without staminodes, 2 carpels, separated to the base, fruit purple-red, with thick glandular points , with a very short beak at the tip. Distributed in sparse forests in Fujian, Guangdong, and Guangxi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 杨成东
Owner NANJING ZELANG AGRI DEV