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Preparation method of protein feed

A protein feed, liquid strain technology, applied in animal feed, animal feed, application and other directions, can solve the problems of waste of resources, low degree of biological utilization, low added value, etc., achieve low cost, high content of viable bacteria, good palatability

Pending Publication Date: 2016-11-09
四川金科药业有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research found that the crude protein content of the oil-extracted meal of Jatropha japonica is 6-9.3%, and the crude fiber content is 21.18%. Low value, serious waste of resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] After activation, Trichoderma lignin and Saccharomyces cerevisiae were respectively inoculated into large test tubes containing 5mL of wort, cultured on a shaking table at 28°C with a rotation speed of 220r / min for 20h, and then inoculated with 5% inoculation amount into 500mL of wort In the Erlenmeyer flask, 28 ℃ shaker culture 20h, according to the mass ratio of Trichoderma and S. Trichotrichum and Bacillus subtilis were respectively inoculated into large test tubes of 5mL wort after activation, cultured on a shaker at 28°C with a rotation speed of 220r / min for 20h, and then inoculated with 5% inoculum in a Erlenmeyer flask containing 500ml wort , cultured on a shaking table at 28°C for 20h, and then inserted into another sterilized liquid strain fermenter at a mass ratio of 1:2 between yeast utilis and Bacillus subtilis and fermented at 30°C for 16h to obtain fermented strains.

[0026] Get the corn soaking water produced in the soaking process in the wet corn starch...

Embodiment 2

[0033] After activation, Trichoderma lignin and Saccharomyces cerevisiae were respectively inoculated into large test tubes containing 5mL of wort, cultured on a shaking table at 28°C with a rotation speed of 220r / min for 20h, and then inoculated with 5% inoculation amount into 500mL of wort In the Erlenmeyer flask, 28 ℃ shaker culture 20h, according to the mass ratio of Trichoderma and S. Trichotrichum and Bacillus subtilis were respectively inoculated into large test tubes of 5mL wort after activation, cultured on a shaker at 28°C with a rotation speed of 220r / min for 20h, and then inoculated with 5% inoculum in a Erlenmeyer flask containing 500ml wort , cultured on a shaking table at 28°C for 20h, and then inserted into another sterilized liquid strain fermenter at a mass ratio of 1:2 between yeast utilis and Bacillus subtilis and fermented at 30°C for 16h to obtain fermented strains.

[0034] Get the corn soaking water produced in the soaking process in the wet corn starch...

Embodiment 3

[0041] After activation, Trichoderma lignin and Saccharomyces cerevisiae were respectively inoculated into large test tubes containing 5mL of wort, cultured on a shaking table at 28°C with a rotation speed of 220r / min for 20h, and then inoculated with 5% inoculation amount into 500mL of wort In the Erlenmeyer flask, 28 ℃ shaker culture 20h, according to the mass ratio of Trichoderma and S. Trichotrichum and Bacillus subtilis were respectively inoculated into large test tubes of 5mL wort after activation, cultured on a shaker at 28°C with a rotation speed of 220r / min for 20h, and then inoculated with 5% inoculum in a Erlenmeyer flask containing 500ml wort , cultured on a shaking table at 28°C for 20h, and then inserted into another sterilized liquid strain fermenter at a mass ratio of 1:2 between yeast utilis and Bacillus subtilis and fermented at 30°C for 16h to obtain fermented strains.

[0042] Get the corn soaking water produced in the soaking process in the wet corn starch...

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Abstract

The invention provides a preparation method of protein feed. The preparation method includes the following steps: inoculating liquid strain into a fermentation substrate for first fermentation to obtain fermentation material; inoculating fermentative strain into the fermentation material for second fermentation so as to obtain the protein feed. The fermentation substrate comprises 75wt%-80wt% of idesia polycarpa cake, 3wt%-5wt% of wheat bran, 10wt%-15wt% of corn steep liquor, 0.5wt%-1wt% of soybean meal, 3wt%-5wt% of rice chaff and 0.6wt%-1wt% of urea; the liquid strain comprises mycete and saccharomyces cerevisiae, and the mycete is selected from trichoderma viride or rhizopus oryzae; the fermentative strain comprises candida utilis and bacillus subtilis. Experiment results show that the protein feed obtained via the method is over 20% in protein content, over 500 million / g in viable count, and good in palatability.

Description

technical field [0001] The invention belongs to the technical field of feed preparation, in particular to a method for preparing protein feed. Background technique [0002] Protein feed refers to feed with a natural moisture content lower than 45%, crude fiber in dry matter lower than 18%, and crude protein content in dry matter reaching or exceeding 20%. With the development of the aquaculture industry, the prices of fishmeal and soybean meal protein feed raw materials continue to rise, which affects the rise in feed prices and the increase in aquaculture costs. The global shortage of feed protein resources forces people to seek and develop new protein resources. [0003] Jatropha magnolia is a high-quality and high-yielding woody oil plant. Its fruit oil content is 43.6%, seed oil content is about 29%, and dried fruit oil yield is about 30%, which is close to that of rapeseed. It is known as a woody oil depot. The content of unsaturated fatty acids such as linoleic acid ...

Claims

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Application Information

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IPC IPC(8): A23K10/12A23K10/10
Inventor 宋艳
Owner 四川金科药业有限责任公司
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