Insulin oral selenium nano preparation and preparation method thereof

A nano-technology of insulin and selenium, which is applied in the direction of pharmaceutical formulations, sulfur/selenium/tellurium active ingredients, medical preparations of non-active ingredients, etc., can solve the problems of weak hypoglycemic effect and low bioavailability, and increase absorption , promote oral absorption, and achieve the effect of treating both symptoms and root causes

Inactive Publication Date: 2016-11-09
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to overcome the disadvantages and deficiencies of low oral bioavailability and weak hypoglycemic effect of insulin in the above-mentioned prior art, the primary purpose of the present invention is to provide an oral selenium nano-preparation of insulin that can promote oral absorption of insulin and has synergistic hypoglycemic effect of selenium

Method used

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  • Insulin oral selenium nano preparation and preparation method thereof
  • Insulin oral selenium nano preparation and preparation method thereof
  • Insulin oral selenium nano preparation and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1: Preparation of Oral Selenium Nano-Preparation for Insulin

[0040]Dissolve insulin in hydrochloric acid solution with pH 2 to prepare insulin solution with a concentration of 5 mg / mL; then dissolve chitosan in 1% acetic acid solution to make its concentration 5 mg / mL; then, prepare 2.5 mg / mL sub Sodium selenate (Na 2 SeO 3 ) solution and 10mg / mL glutathione (GSH) solution. Take 10 mL of chitosan solution, slowly add 5 mL of insulin solution dropwise under stirring, and incubate for 15 min. Then, slowly add Na 2 SeO 3 Solution 5mL, continue to incubate for 1h. Then slowly add 5 mL of GSH solution and react for 1 hour to obtain a red transparent and opalescent insulin-loaded selenium nanoparticle suspension.

Embodiment 2

[0041] Example 2: Preparation of Oral Selenium Nano-Preparation for Insulin

[0042] Dissolve insulin in hydrochloric acid solution at pH 2 to prepare a 5 mg / mL insulin solution; then dissolve chitosan in 1% acetic acid solution to make the concentration 5 mg / mL; then prepare 2.5 mg / mL selenium Sodium acid (Na 2 SeO 4 ) solution and 10mg / mL glutathione (GSH) solution. Take 10 mL of chitosan solution, slowly add 5 mL of insulin solution dropwise under stirring, and incubate for 15 min. Then, slowly add Na 2 SeO 4 Solution 5mL, continue to incubate for 1h. Then slowly add 5 mL of GSH solution and react for 1 hour to obtain a red transparent and opalescent insulin-loaded selenium nanoparticle suspension.

Embodiment 3

[0043] Example 3: Preparation of Oral Selenium Nano-Preparation for Insulin

[0044] Dissolve insulin in hydrochloric acid solution with pH 2 to prepare insulin solution with a concentration of 5 mg / mL; then dissolve chitosan in 1% acetic acid solution to make its concentration 5 mg / mL; then, prepare 2.5 mg / mL sub Sodium selenate (Na 2 SeO 3 ) solution and 10mg / mL vitamin C solution. Take 10 mL of chitosan solution and place it on a magnetic stirrer, slowly add 5 mL of insulin solution dropwise, and incubate for 15 min. Then, slowly add Na 2 SeO 3 Solution 5mL, continue to incubate for 1h. Slowly add 5 mL of vitamin C solution and react for 1 hour to obtain a red transparent and opalescent insulin-loaded selenium nanoparticle suspension.

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Abstract

The invention belongs to the technical field of medicinal preparations, and discloses an insulin oral selenium nano preparation being capable of promoting the oral absorption of insulin and having the synergetic blood sugar decreasing effect of selenium, and a preparation method of the insulin oral selenium nano preparation. According to the preparation method, a selenium-containing reagent is added into a solution or suspension containing insulin and chitosan, and selenium/chitosan nanoparticles loaded with insulin are generated under the effect of a reducing agent. The insulin oral selenium nano preparation can be effectively loaded with insulin, so that insulin is efficiently loaded into the nanoparticles formed by chitosan and selenium, therefore, the release and exposure of insulin in the gastrointestinal tract after oral medication are reduced, thus the gastrointestinal tract stability of insulin is improved, the absorbability and biological activity of selenium are also improved, and finally, the bioavailability of insulin and selenium is improved. In addition, with the addition of selenium, the preparation has the effect of decreasing blood sugar level, meanwhile, the function of the body can be reconstructed through the oxidation resistance, blood sugar regulation and insulin-mimetic effect of selenium, the synergetic blood sugar decreasing effect is realized through the insulin-mimetic effect of selenium, and thus the function of treating both symptoms and root causes of diabetes is realized.

Description

technical field [0001] The invention belongs to the technical field of pharmaceutical preparations, and in particular relates to an oral selenium nano-preparation for insulin capable of promoting oral absorption of insulin and having synergistic hypoglycemic effect of selenium and a preparation method thereof. Background technique [0002] Diabetes is a common and frequently-occurring disease that seriously endangers human health. In recent years, the incidence of diabetes has been on the rise. According to WTO estimates, the incidence of diabetes in adults is as high as 9%. Insulin is the first-line drug for the treatment of insulin-dependent type I diabetes, and it is also the adjuvant drug for the treatment of advanced type II diabetes. At present, subcutaneous injection is the most basic way of administering insulin. However, there are many disadvantages in administration via subcutaneous injection, such as frequent administration, painful injections, large blood sugar...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/28A61K9/16A61K47/48A61P3/10A61K33/04
CPCA61K38/28A61K9/1652A61K9/167A61K33/04A61K2300/00
Inventor 张兴旺吴宝剑邓雯姬
Owner JINAN UNIVERSITY
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