Recombinant Newcastle disease heat-resistant vaccine strain for expression of signal peptide-replaced H5 subtype avian influenza virus HA protein and preparation method thereof

Abstract

The invention discloses a recombinant Newcastle disease heat-resistant vaccine strain for expression of a signal peptide-replaced H5 subtype avian influenza virus HA protein and a preparation method thereof. The heat-resistant vaccine strain is named as rTS-tHA/H5, is preserved in the Wuhan university China center for type culture collection on April 19, 2016 and has an accession number of CCTCC NO: V201628. After infection of animals or cells with the vaccine strain, the vaccine strain can highly express a H5 subtype avian influenza virus truncated HA protein and the tPAs of the vaccine strain can improve secretory expression and immunogenicity of the truncated HA protein. The vaccine strain can be used for preparation of a chicken Newcastle disease and H5 subtype avian influenza bivalent heat-resistant living vaccine.

Description

technical field [0001] The invention belongs to the field of molecular biology, and specifically relates to a recombinant Newcastle disease heat-resistant vaccine strain expressing signal peptide-substituted H5 subtype avian influenza HA protein and a preparation method thereof. Background technique [0002] H5 subtype avian influenza virus can cause an acute fatal infectious disease of poultry - highly pathogenic avian influenza, its clinical manifestations are high mortality, decreased egg production, and severe respiratory symptoms. H5 subtype avian influenza can also infect humans and cause disease. Inoculation of inactivated vaccines is the main means of prevention and control of H5 subtype avian influenza in my country. Traditional inactivated vaccines are relatively simple to prepare, convenient to store and transport, and have long-lasting immune effects. They have played an important role in the prevention and control of avian influenza. However, inactivated vacci...

AI Technical Summary

Problems solved by technology

[0005] The existing H5 avian influenza and Newcastle disease dual vaccines that have been on the market are all prepared after inactivating the H5 subtype avian influenza virus strain and the Newcastle disease virus strain. The main reason is that the H5 subtype avian influenza virus has a certain pathogenicity and cannot be used in the preparation of live vaccines

The disadvantage of this type of vaccine is that it requires intramuscular injection, which is labor-intensive and time-consuming, and the cost of immunization is high

The use of inactivated vaccines has increased the difficulty of clinically distinguishing wild virus infection from vaccine immunity to a certain extent, and there is a risk of spreading the virus

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