Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for rapidly identifying resistance of peanut varieties to aspergillus flavus

A technology of Aspergillus flavus and Aspergillus flavus spores, which is applied in the field of rapid identification of peanut varieties against Aspergillus flavus, and achieves the effects of improving efficiency, high equipment requirements and simple operation.

Inactive Publication Date: 2016-11-16
BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
View PDF4 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] The invention aims at the defects of the existing methods for identifying the resistance of peanuts to Aspergillus flavus infection, and provides a method for quickly and accurately identifying the resistant varieties of peanuts resistant to Aspergillus flavus

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for rapidly identifying resistance of peanut varieties to aspergillus flavus
  • Method for rapidly identifying resistance of peanut varieties to aspergillus flavus
  • Method for rapidly identifying resistance of peanut varieties to aspergillus flavus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Embodiment 1: the cultivation of Aspergillus flavus and the collection of spores

[0056] (1) Streak and activate the Aspergillus flavus strains on the PDA solid medium, and cultivate them in a dark incubator at 30°C for 6 days;

[0057] The components per liter of PDA solid medium are as follows:

[0058] Potato 300g, glucose 20g, agar 20g, pH value natural.

[0059] (2) After Aspergillus flavus hyphae cover the surface of the culture medium, wash it down with sterilized distilled water, resuspend in sterile water, calculate its concentration with a hemocytometer, and adjust the spore concentration to 10 6 per ml, as a suspension of Aspergillus flavus spores for later use.

Embodiment 2

[0060] Embodiment 2: the processing of peanut seed

[0061] (1) Take 15 seeds each of peanut varieties Yueyou 20, Tifrunner, Luhua No. 14, Jihua No. 11, Zhonghua No. 5, Yuhua No. 6, and Zhonghua No. 6 healthy peanut plants, peel off the peel, and take Seeds with intact seed coats are used as samples and divided into Erlenmeyer flasks (if the seeds are dried, the seeds need to be soaked in sterile water and fully absorbed);

[0062] (2) add mercuric chloride of mass concentration 0.1% to disinfect for 10 minutes, shake at 100 r / min during this period, then rinse three times with distilled water, and rinse at 100 r / min for 5 minutes during the third rinse, and then make 6mm×5mm wounds on the seeds, Remove the seed coat from the wounded part.

Embodiment 3

[0063] Embodiment 3: Inoculation of Aspergillus flavus

[0064] (1) Cover the wound of the seed in Example 2 with a sterile tampon of the same size, drop 20 microliters of the Aspergillus flavus spore suspension prepared in Example 1 on the tampon, and distribute it in numbered sterile Bacteria culture dish for cultivation;

[0065] (2) Fold two layers of absorbent paper and cut it into a circle and lay it in a petri dish. Add 5ml of sterilized distilled water to each petri dish to moisten the toilet paper so that the water content in the petri dish reaches 80%. Five seeds, 3 replicates for each sample;

[0066] (3) Place the petri dish in an incubator, culture in dark at 30° C. for 24 hours, remove the cotton sliver, and culture in dark for 144 hours.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a method for rapidly identifying resistance of peanut varieties to aspergillus flavus. Fresh or sun-dried peanut seeds are subjected to surface sterilization, wounds are made on peanut coats after sterilization, inoculation is performed, and peanuts are kept moist by the aid of cotton balls after inoculation and then put in an incubator at the temperature of 30 DEG C for dark culture; after one week of dark culture, the resistance of the peanut varieties to aspergillus flavus is evaluated by calculating the infection indexes of aspergillus flavus. Compared with a method adopting weighing of biomass of aspergillus flavus after inoculation, the preparation method has the advantages that errors of the water content of peanut seeds cannot be considered, and the evaluation result is more accurate and stable.

Description

technical field [0001] The invention relates to a method for quickly identifying the resistance of peanut varieties to Aspergillus flavus, and belongs to the technical field of plant germplasm identification. Background technique [0002] Peanut is an important economic crop, oil crop and foreign exchange earning crop in my country. The annual planting area of ​​peanut in China is close to 5 million hectares, and the annual output is about 17 million tons. my country's peanut planting area accounts for 20% of the world's total planting area, ranking second in the world; its output accounts for about 40% of the world's total output, ranking first in the world. However, peanuts are prone to be polluted by Aspergillus flavus during field management, harvesting, drying, storage, transportation and processing to produce aflatoxin, which causes great harm to human health and seriously affects the quality of peanuts and their products in my country. , has become a barrier to my cou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/67
CPCC12Q1/18
Inventor 王兴军赵传志李婷婷夏晗侯蕾马德源李爱芹仇静静赵术珍李膨呈
Owner BIOTECH RES CENT SHANDONG ACADEMY OF AGRI SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com