Novel glutamic acid extracting process

An extraction process, glutamic acid technology, applied in the preparation of organic compounds, methods based on microorganisms, biochemical equipment and methods, etc., can solve the problems of low conversion rate, high consumption of sulfuric acid and liquid ammonia, high cost of culture medium, etc. Achieve the effects of improved strain utilization, low consumption of sulfuric acid, and low cost

Active Publication Date: 2016-11-23
HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the defects of the prior art, such as high cost of fermentation medium, low conversion rate, high consumption of sulfuric acid and liquid ammonia, etc., and provides a new glutamic acid extraction process

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A new glutamic acid extraction process, which comprises the steps of:

[0028] 1) Weigh the raw materials of the fermentation medium: take each raw material according to the weight percentage for later use, including: 7% wheat bran hydrolyzate, 4% glucose, 3% straw treatment, 1% rice bran extract, 0.05% urea, and 0.02% shell powder , magnesium sulfate 0.01%, potassium dihydrogen phosphate 0.01%, and the rest is water;

[0029] 2) Preparation of fermentation medium: Add wheat bran hydrolyzate, glucose, straw treatment, rice bran extract, urea, shell powder, magnesium sulfate and potassium dihydrogen phosphate to water in sequence, stir evenly, and then maintain the Sterilize for 8 minutes, then cool down to 32°C to prepare a fermentation medium;

[0030] 3) Fermentation: Put the temperature-sensitive glutamic acid strain Brevibacterium flavum TMG0106 seed culture solution in the logarithmic growth phase into the fermentation medium at a volume ratio of 10% of the seed c...

Embodiment 2

[0042] Comparative test of process acid production rate and sugar-acid conversion rate of the present invention:

[0043] Control group: fermentation medium used: glucose 5%, yeast extract 6%, peptone 7%, corn steep liquor 1%, magnesium sulfate 1%, urea 0.05%, ferrous sulfate 0.03%, magnesium sulfate 0.02%, potassium dihydrogen phosphate 0.01%, VB1 0.01%, VH 0.01%, and the rest are water; the fermentation process is the same as in Example 1.

[0044] Embodiment 1 of the present invention and control group: the acid production rate and sugar-acid conversion rate of each group are shown in Table 1:

[0045] Table 1

[0046] group

[0047] Conclusion: The amount of acid production and the sugar-acid conversion rate of the Example 1 group of the present invention and the control group are not much different, and the Example group of the present invention is slightly higher; the cost of the culture medium of the Example 1 of the present invention only accounts for the cu...

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PUM

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Abstract

The invention belongs to the technical field of amino acid fermentation extraction, and discloses a novel glutamic acid extracting process. The glutamic acid extracting process comprises the following steps: 1) weighing raw materials of a fermentation medium, 2) preparing the fermentation medium, 3) performing fermentation, 4) performing membrane filtration, and 5) performing evaporation concentration and isoelectric crystallization. By the glutamic acid extracting process provided by the invention, the used fermentation medium is low in cost, the conversion rate is high, the consumption of sulfuric acid and liquid ammonia is relatively low, and the industrial added value is increased.

Description

technical field [0001] The invention belongs to the technical field of amino acid fermentation and extraction, and in particular relates to a new glutamic acid extraction process. Background technique [0002] Glutamic acid is an acidic amino acid. The molecule contains two carboxyl groups, and its chemical name is α-aminoglutaric acid. Glutamic acid was discovered by Li Suxun in 1856. It is a colorless crystal with umami taste, slightly soluble in water, but soluble in hydrochloric acid solution, with an isoelectric point of 3.22. A large amount exists in cereal protein, and the content in animal brain is also more. Glutamic acid plays an important role in the protein metabolism process in organisms, and participates in many important chemical reactions in animals, plants and microorganisms. Sodium glutamate, commonly known as monosodium glutamate, is an important umami agent that enhances the fragrance. Sodium glutamate is widely used as a food flavoring agent, eithe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/14C07C227/40C07C227/42C07C229/24C12R1/13
Inventor 杨雪尤学波王刚许传高杜鹏飞
Owner HULUNBEIER NORTHEAST FUFENG BIOTECHNOLOGIES CO LTD
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