Supercharge Your Innovation With Domain-Expert AI Agents!

Method for increasing frequency of capsicum somatic embryogenesis

A technology of somatic embryos and occurrence frequency, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of easy vitrification, low occurrence frequency, long cycle, etc. The effect of improving induction rate and maturation rate

Active Publication Date: 2016-12-07
HUNAN VEGETABLE RES INST
View PDF2 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are still many problems in the occurrence of somatic embryos in peppers, mainly due to the long cycle, low frequency of occurrence, and easy vitrification, etc. The current embryo induction cycle is about 200 days, and the occurrence frequency is less than 1%.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for increasing frequency of capsicum somatic embryogenesis
  • Method for increasing frequency of capsicum somatic embryogenesis
  • Method for increasing frequency of capsicum somatic embryogenesis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] (1) Disinfection and sowing: take the dry seeds of pepper (Zhangshugang), disinfect with 0.1v / v% mercuric chloride for 10min, rinse with sterile water 5 times, and set aside;

[0040] (2) Acquisition of sterile seedlings: place the sterilized seeds obtained in step (1) in an ultra-clean workbench, and inoculate them into MS with a pH value of 5.8. 1 Cultivate in the medium for 12 days at 25°C under 8 / 16h light conditions to obtain sterile seedlings;

[0041] (3) Selection of explants and induction of callus: under aseptic conditions, the cotyledons and hypocotyls of the aseptic seedlings in step (2) were placed in MS with a pH value of 5.8 2 In the culture medium, culture at a culture temperature of 25°C and in the dark for 12 days to obtain callus tissue;

[0042] (4) Induction of somatic embryos: under aseptic conditions, select embryogenic callus from the callus obtained in step (3), cut the callus with cotyledons and hypocotyls, and transfer to MS at pH 5.43 The c...

Embodiment 2

[0060] Prepare callus induction medium and weigh it, denoted as M 1 Get two seedlings whose cotyledons have just flattened out, cut their cotyledons and hypocotyls into blades and 0.5cm long stems of about 0.5cm in length and width respectively, and place them in MS 2 Induced and weighed, denoted as M 2 ; Weigh the weight M of the medium at the 10th, 11th, 12th, 13th, 14th and 15th day respectively 3 . Then the callus induction rate is: (M 3 -M 2 )÷(M 2 -M 1 ) × 100%; the induction rate of embryogenic callus: the number of embryogenic callus pieces ÷ the number of callus pieces × 100%

[0061] Table 1 The effect of different induction time on the callus induction rate of cotyledons and hypocotyls

[0062]

[0063]

[0064] Note: Although the induction rate of callus increases with the prolongation of induction time, the induction rate of embryogenic callus begins to decrease from 12 days, so the optimal induction time of cotyledons and hypocotyls is 11-13 days.

...

Embodiment 4

[0070] The cotyledons of the 12d seedling age were selected as explants, and the MS containing different concentrations of sucrose 2 Induce callus 12d, repeat three times for each concentration, calculate the induction rate of embryogenic callus; select 10 pieces of embryogenic callus with similar quality (each weight is about 10mg), in the MS containing different concentrations of sucrose 3 Induce somatic embryos in medium, repeat three times for each concentration, calculate the induction rate of somatic embryos, that is, the number of somatic embryos induced by each callus; place similar somatic embryos in MS containing different concentrations of sucrose 4 Medium amplification for one month, each concentration was repeated three times, and the expansion rate of somatic embryos was calculated, that is, the number of single somatic embryos amplified within the same time period; somatic embryos with a similar number were placed in MS containing different concentrations of sucr...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for increasing the frequency of capsicum somatic embryogenesis. The method comprises the steps of disinfecting and sowing seeds to obtain aseptic seedling, selecting explant, inducing a callus tissue, inducing somatic embryo, subculturing and reproducing the somatic embryo, and maturing the somatic embryo, wherein the explant is cotyledon or hypocotyledonary axis; the process from inducing the callus tissue to maturing somatic embryo is carried out under a dark condition. By adopting the method, the capsicum somatic embryogenesis time is shortened, the quantity of embryogenesis is increased, and the somatic embryogenesis efficiency is improved, so that foundation is laid for capsicum transgenic researches, biotechnological breeding and the like.

Description

technical field [0001] The invention belongs to the field of occurrence of pepper somatic embryos, and in particular relates to a method for increasing the occurrence frequency of pepper somatic embryos. Background technique [0002] Capsicum is an annual or limited perennial herbaceous plant belonging to the genus Capsicum in the family Solanaceae, native to Mexico, and is an important vegetable crop widely cultivated worldwide. "Compendium Supplements" records: spicy eggplants are hot and loose, and can also remove dampness; "Baicao Mirror" also pointed out that peppers can wash chilblains, bath cold scabies, and relieve cold addiction in the large intestine, which has certain medicinal value. [0003] As a crop with important economic value, pepper has strict requirements on water content, that is, it is not resistant to drought or waterlogging, and some traits need to be improved. At present, pepper is mainly reproduced through sexual reproduction, but traditional breed...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/005
Inventor 邹学校刘峰张亚利戴雄泽马艳青李雪峰郑井元
Owner HUNAN VEGETABLE RES INST
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com