Micro-ecological vaginal bacterial agent as well as preparation method and application thereof

A micro-ecological and vaginal technology, applied in the field of bacterial preparations for the prevention and treatment of vaginal diseases, can solve problems such as unsatisfactory treatment effects, recurrent gynecological diseases, vaginal flora disorder, etc., achieve low incidence of adverse reactions, enhance vaginal Self-cleaning function, the effect of enhancing the body's immunity

Inactive Publication Date: 2016-12-07
汪金小
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  • Summary
  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, whether it is pure traditional Chinese medicine or a combination of traditional Chinese medicine and chemical ingredients, the abuse of these bactericidal substances will seriously damage the vaginal self-cleaning system, directly lead to vaginal flora disorder, make gynecological diseases prone to relapse, and the treatment effect is not ideal

Method used

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preparation example Construction

[0038] The present invention also provides the preparation method of described micro-ecological vaginal bacterial agent, comprising the following steps:

[0039] 1) Inoculate the mixed strain of Lactobacillus crispatus and Lactobacillus jansseni on the enrichment medium for enrichment culture to obtain the enrichment culture solution;

[0040] 2) inoculating the enriched culture solution obtained in the step 1) on the expansion medium to expand the culture to obtain the expansion culture solution;

[0041] 3) Inoculate the expanded culture solution obtained in the step 2) on the fermentation medium for fermentation and culture, and the obtained fermentation solution is a micro-ecological vaginal bacterial agent.

[0042] The preparation method of the micro-ecological vaginal bacterial agent provided by the present invention has the advantages of simple method, good repeatability and low cost.

[0043] In the present invention, the mixed strains of Lactobacillus crispatus and La...

Embodiment 1

[0087] Wash the earthworms with living water, add brown sugar (also known as cane sugar) at a ratio of 5:3 between earthworms and brown sugar, wait for the earthworms to dissolve, centrifuge the earthworm liquid, take the supernatant, dilute it with acetic acid buffer, and pour it into In a boiling water bath, stir for 5 minutes while heating, then centrifuge, take the supernatant, dilute it with acetic acid buffer, filter it with an ultrafiltration system with a molecular weight of 10,000Da or separate it with a high-speed centrifuge to obtain an earthworm antimicrobial peptide solution.

Embodiment 2

[0089] Wash the earthworms with living water, add brown sugar (also known as sugarcane sugar) at a ratio of earthworms to brown sugar 4:3.5, wait for the earthworms to dissolve, centrifuge the earthworm liquid, take the supernatant, dilute it with acetic acid buffer, and pour it into In a boiling water bath, stir while heating for 8 minutes, then centrifuge, take the supernatant, dilute it with acetic acid buffer, filter it with an ultrafiltration system with a molecular weight of 10,000Da or separate it with a high-speed centrifuge to obtain an earthworm antimicrobial peptide solution.

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Abstract

The invention provides a micro-ecological vaginal bacterial agent. The agent is prepared from a fermented liquid prepared from Lactobacillus crispatus and Lactobacillus jensenii through compatible fermentation. The invention further provides a preparation method of the micro-ecological vaginal bacterial agent. According to the method, Lactobacillus crispatus and Lactobacillus jensenii are mixed to be compatible, an enrichment culture medium is inoculated with the mixed bacterial liquid for enrichment culture, and an enrichment culture liquid is obtained; an enlargement culture medium is inoculated with the enrichment culture liquid for enlargement culture, and an enlargement culture liquid is obtained; a fermentation culture medium is inoculated with the enlargement culture liquid for fermentation culture, and the obtained fermented liquid is the micro-ecological vaginal bacterial agent. The micro-ecological vaginal bacterial agent is applied to preparation of drugs for preventing and treating gynecological diseases such as recurrent vaginitis or discharge abnormality. With the micro-ecological vaginal bacterial agent, the cure rate ranges from 88% to 98%, the effective rate ranges from 92% to 100%, the recurrence rate within three months ranges from 0 to 6%, and the adverse reaction rate is low, thereby proving that the micro-ecological vaginal bacterial agent is effective in treatment of various recurrent vaginitis.

Description

technical field [0001] The invention relates to the technical field of bacterial agents for preventing and treating vaginal diseases, in particular to a probiotic vaginal bacterial agent and its preparation method and application. Background technique [0002] According to statistics from authoritative departments, the prevalence rate of gynecological diseases among adult females in my country is as high as 87.5%. In elderly women, due to ovarian function decline, estrogen levels decrease, vaginal wall atrophy, mucous membranes become thinner, glycogen content in epithelial cells decreases, vaginal pH increases, local resistance decreases, and pathogenic bacteria are easy to invade and reproduce and cause inflammation. Gynecological inflammation includes female vulvitis, vaginitis, cervicitis, pelvic inflammatory disease, etc. If it is not prevented and treated in time, it may cause other diseases, such as affecting the upward movement of sperm, hindering the combination of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/747A61P15/02A61P15/00C12N1/20C12R1/225
CPCA61K35/747C12N1/20A61K2300/00
Inventor 汪金小汪紫燕
Owner 汪金小
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