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A rapid determination method for rice with high dietary fiber

A technology with high dietary fiber and determination method, which is applied in the preparation of test samples, instruments, measuring devices, etc., can solve the problems of difficult determination of grain dietary fiber, screening of unfavorable materials, and complicated operation, and achieves low detection cost and savings. Labor and time cost, the effect of accurate results

Active Publication Date: 2019-01-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The above determination method has the disadvantages of time-consuming, cumbersome operation, high cost, etc., and the determination of grain dietary fiber is easily interfered by starch, so the determination of grain dietary fiber is difficult, which is not conducive to the screening of materials in breeding work.

Method used

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  • A rapid determination method for rice with high dietary fiber
  • A rapid determination method for rice with high dietary fiber
  • A rapid determination method for rice with high dietary fiber

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A rapid determination method for high dietary fiber rice, specifically comprising the following steps:

[0028] (1) Get high dietary fiber mutant rice seeds and ordinary rice Nipponbare seeds, peel and polish into polished rice;

[0029] (2) Take 100mg of polished rice grains in a test tube, add 1mL of water, and cook in boiling water until edible;

[0030] (3) Remove excess water, add n-hexane to the test tube, and perform repeated extraction until Sudan III cannot detect fat;

[0031] (4) Remove the extract, dry the solid phase, add 2 mL of KCl-HCl buffer solution (0.1M, pH=1.5) containing 200 IU of protease to the test tube, place the test tube in a water bath shaker at 37°C (160rpm ), proteolysis reaction for 2 hours, until Ponceau S can detect no protein residue;

[0032] (5) Remove the buffer of the proteolysis reaction, add 4mL sodium maleate buffer solution (0.1M, pH=6.5) containing 200IU α-amylase and 15IU amyloglucosidase to the test tube, keep the temperatu...

Embodiment 2

[0041] In order to further expand the sample size, this embodiment judges the level of dietary fiber content in the stable line obtained by crossing the high dietary fiber mutant and common rice Nipponbare, and compares it with the results of dietary fiber measured by the standard method (AOAC991.43). Compared.

[0042] Material acquisition: cross the high dietary fiber mutant with the low dietary fiber mutant to obtain F 1 generation plant, F 1 F 2 generation plant, F 2 Afterwards, the strains were continuously selfed by the single-seed method, and 130 strains with stable dietary fiber phenotype were obtained.

[0043] Adopt the method described in embodiment 1 to measure the morphological characteristics after the milled rice grain of above-mentioned 130 strains is processed; Adopt the dietary fiber content of the rice of 130 strains to measure by AOAC991.43 method; Measurement result is as follows figure 2 shown.

[0044] The result is as follows: by figure 2 As sho...

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Abstract

The invention discloses a method for rapidly determining high dietary fiber rice. The method comprises the following steps: (1) taking polished rice grains and cooking; (2) sequentially removing fat, protein and starch in the cooked rice grains by organic solvent extraction, protease hydrolysis and amylase hydrolysis to obtain residue; (3) by observing the form of the residue with the naked eyes, determining that the residue which can retain the intact grain form is high dietary fiber rice, and the residue which becomes white loose floccules without a grain structure is low dietary fiber rice, wherein the dietary fiber content of the high dietary fiber rice is more than or equal to 5 percent. The cooked polished rice grains are taken as a study object, the fat, the protein and the starch in the rice grains are removed by a relatively mild treatment method, and the content of the dietary fiber in the rice is determined by the appearance form of the rice grains; the method not only is simple and efficient, but also the results are accurate.

Description

technical field [0001] The invention relates to the field of nutritional evaluation of functional staple foods, in particular to a rapid determination method for rice with high dietary fiber. Background technique [0002] Dietary fiber refers to the general term of edible plant components or homologous carbohydrates that cannot be digested and absorbed in the small intestine, but can be completely fermented and degraded or partially degraded in the large intestine. It is divided into two categories: insoluble and water-soluble fibers . Dietary fiber contains low energy, which can control the cholesterol and blood sugar in the blood at an ideal level, and has many benefits to the human body. If the dietary fiber intake of the human body is insufficient, the incidence of diseases such as constipation, colon cancer, appendicitis, diabetes, obesity, coronary heart disease, and gallstones will increase. Today, as the diet is becoming more and more refined, the intake of dietary...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/84G01N1/34
CPCG01N1/34G01N21/84
Inventor 孙健吴殿星舒小丽张宁袁名安
Owner ZHEJIANG UNIV
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